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Citations for Greiner :
701 - 750 of 3126 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The cultures were then diluted to obtain a final OD600nm=0,01 using the medium of choice at a final volume of 150 μl per well in black 96-well plates with an optical flat bottom (Greiner) and ...
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bioRxiv - Biochemistry 2023Quote: ... introduced by the Sleeping Beauty transposon system,82 were seeded at a density of 1200 cells per well in a 384 well plate (Cell culture microplate, PS, f-bottom, µClear, 781091, Greiner) in culture medium (50 µL per well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were seeded at a density of 1,200 cells/well in 384 well plates in culture medium (cell culture microplate, PS, f-bottom, µClear®, 781091; Greiner). Cell confluence was measured using the IncuCyte® S3 (Satorius ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were seeded at a density of 1,500 cells/well in 384 well plates in culture medium (cell culture microplate, PS, f-bottom, µClear®, 781091; Greiner). Cells were stained simultaneously with 60 nM Hoechst33342 (Thermo Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µL of the suspension of the co-cultured cells was transferred to 96-well white flat bottom TC plate (Greiner) and 50 µL of the working solution of the QUANTI1Luc 4 Lucia/Gaussia reagent (Invivogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the worms were transferred to the wells of a black wall/clear bottom 384-well plate (Greiner Bio-One 781986). Using a multichannel pipette ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used the 96-pin replication tool to deposit cells from a 384 array quadrant in 96-well flat bottom plates (Greiner) filled with sterile water ...
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bioRxiv - Microbiology 2024Quote: ... Cells were counted using a haemocytometer and 105 cells were seeded into each well of a sterile 96-well flat-bottom polystyrene microtiter plate (Greiner) in a total volume of 100 μL ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1.5 pmol of siRNA (4 μl) was dispensed into each well of black clear-bottom 384-well plates (Greiner, 781091) with a Biomek FXP liquid handler (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at 10,000 cells per well in V-bottom 96-well low-cell-binding plates (Greiner Bio-One) in DB27 medium (DMEM/F12 HEPES ...
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bioRxiv - Microbiology 2022Quote: ... A 200μL aliquot of inoculated organic acid solution for each strain was transferred to a 96-well U-Bottom plate (Greiner), in triplicate wells ...
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bioRxiv - Microbiology 2022Quote: 25 μl of ferret sera or human mAbs were two-fold serially diluted in 96-well V-bottom plates (Greiner) with PBS prior to being mixed with equal volume of H7N9 Anhui/13 (8 HA units) ...
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bioRxiv - Microbiology 2022Quote: ... 50 μl of virus was two-fold serially diluted and added to the 96-well V- bottom shaped plates (Greiner), followed by addition of 50 μl of 1% chicken RBCs ...
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bioRxiv - Synthetic Biology 2022Quote: ... clear bottom 96-well plates (Cell Culture Microplates 96 Well μClear® CELLSTAR®, Greiner Bio-One GmbH, Product #: 655090), which was sealed with peelable foil (Sealing foil ...
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bioRxiv - Systems Biology 2022Quote: ... oneidensis knockout collection were replicated from a master collection (stored at -80 °Cwith a pin-tool (EnzyScreen CR1000) into a flat-bottom polypropylene plate (Part no. 655261, Greiner) containing 150 μL of LB media per well with 30 mg L-1 Kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl Nano-Glo HiBiT Lytic Reagent containing 1 μl furimazine and 2 μl recombinant LgBiT were added to 50 μl lysate in a white flat-bottom 96 well plate (Greiner) in technical triplicates ...
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bioRxiv - Cancer Biology 2024Quote: ... the PDMS layer was chemically glued and subjected to 2 minutes of oxygen plasma treatment to affix it to the bottom of a bottomless 96-well plate (Greiner). Following this ...
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bioRxiv - Microbiology 2024Quote: ... with or without a 6 mm porcine valve punch biopsy in either sterile flat-bottom 96-well polystyrene tissue culture plates (CellStar; Greiner Bio-One ...
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bioRxiv - Immunology 2024Quote: ... 25 µl of the resulting supernatant was mixed with 75 µl FS in 96 half well flat bottom plates (Greiner) and absorbance was measured at 562 nm (Infinite® 200 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs (5 × 105) were cultured in a final volume of 200 μL/well in round-bottom 96-well plates (Greiner) and stimulated with RPMI 1640 (medium control) ...
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bioRxiv - Biochemistry 2024Quote: ... 80 µl of the assayed solution were transferred to a transparent 96-well flat-bottom plate (Greiner Bio-One, Germany). Kd and IC50 determinations for compounds 20 ...
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Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approachbioRxiv - Synthetic Biology 2024Quote: ... culture pellets were washed and re-suspended in 500 µl PBS before being transferred to black clear bottom microtiter plates (BMTPs; Greiner). All density and fluorescence readouts were performed in a ClarioStar microplate reader (BMG) ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were diluted to ∼20 nM at least an hour before measurement in a flat-bottom 96-well polypropylene plate (Greiner). After centring the laser over a well on a 24-well gasket on commercially pre-cleaned slides (Refeyn) ...
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bioRxiv - Biochemistry 2024Quote: ... were combined with the equal volume of 2-fold dilution series of the degraders or JQ1 (prepared in DMSO and then diluted with the binding buffer to reach the final DMSO concentration of 2% for all titration points) and incubated for 1 h at room temperature before transferring to 384-well flat bottom black plates (Greiner). Fluorescence polarization was calculated by measuring perpendicular and parallel fluorescence intensity values using the excitation and emission wavelengths of 482 nm and 530 nm ...
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bioRxiv - Microbiology 2023Quote: ... flat bottom microplates (Greiner) were filled with 200 µl of culture in triplicate ...
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bioRxiv - Microbiology 2022Quote: ... flat-bottom microplates (Greiner). Assay mixtures (100 µL ...
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bioRxiv - Cell Biology 2022Quote: ... or onto PLL-Laminin coated glass coverslips (Marienfeld-Superior #0111520) in 24 well plates (Greiner Bio-One #66210). Glass coverslips had been previously plasma cleaned for 5 min each side using O2 plasma ...
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bioRxiv - Synthetic Biology 2021Quote: ... used to inoculate experimental cultures at 100x dilution in 200 µL volumes in a Greiner clear bottom 96 well plate (Greiner 655090) in LBL media ...
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bioRxiv - Microbiology 2020Quote: ... were inoculated with 2 µl of the respective pre-culture adapted to the respective NaCl concentration in a sterile 96-well microtiter plate with optical bottom (Greiner, Germany) and incubated in an automated microplate reader (Tecan ...
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bioRxiv - Cancer Biology 2022Quote: ... This mixture (1 mL) was applied to the bottom of an ultra-low attachment 6-well plate (Greiner Bio-One Inc.) and allowed to cool for 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... in a total reaction volume of 180 μL using a 96-well μClear bottom black plate (Greiner Bio-One, Monroe, NC). Samples were subject to excitation at 450 nm and emission at 490 nm at 37°C and fluorescence intensity was measured every five min for 24 hr using an MX Synergy microplate reader ...
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bioRxiv - Cell Biology 2022Quote: ... 100 µL aqueous solutions containing 50 mM MES pH 5.5 were added to 100 µL of cell suspension in 96-well flat-bottom plates (#655101; Greiner, Monroe, NC). Osmolyte titration curves were analyzed using the GraphPad Prism software ...
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bioRxiv - Immunology 2022Quote: ... Each sample was tested in triplicate in white 96-well plates with clear bottoms (LUMITRAC 200, Greiner Bio-One, Monroe, NC) with 5 times of volume to the supernatant sample ...
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bioRxiv - Biochemistry 2022Quote: ... 0.01 % (v/v) Triton X-100 and 0.5 mM TCEP) in a 384-well black polypropylene flat-bottom plates (Greiner, Bio-one) with 10 µL reaction volume per well ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated with a density of 2 × 105 cells/ml into white clear-bottom 6-well plates (Greiner Bio-One) 2 ml per well for inductively coupled plasma mass spectrometry (ICP-MS ...
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bioRxiv - Molecular Biology 2020Quote: ... some oocytes were harvested for T = 0 samples and the remaining oocytes were incubated in kulori buffer (pH 7.4) in a 96-well U-bottom microtiter plate (Greiner Bio-One) (3 oocytes in a well containing 200 μl buffer) ...
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bioRxiv - Biochemistry 2021Quote: Measurements were taken in a 96-well black plate (Microplate 96 Well PS F-Bottom Black Non-Binding, Greiner Bio-one) using a TECAN M1000 Pro ...
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bioRxiv - Microbiology 2021Quote: ... Eighty microliters of the glycerol stock were then aliquoted into two V-bottom 96-well plates (Greiner Bio-One, Cat. #651161). The cryo-stock plates were sealed with a foil seal and stored at -80 °C ...
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bioRxiv - Microbiology 2020Quote: SupT1-R5 cells were distributed in well of 96-well plate (4 × 105 cells/well; U-bottom; Greiner Bio-One, 650180), pelleted (3 min / 200 × g ...
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bioRxiv - Immunology 2021Quote: ... The cell pellets were resuspended in 100μL of PBS and transferred to a 96-well plate with V-bottom tubes (Greiner bio-one). The V-bottom plate was centrifuged for 10 minutes at RT/3200rpm ...
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bioRxiv - Synthetic Biology 2023Quote: ... cultures were prepared from confluent overnight cultures used to inoculate experimental cultures at 100x dilution in 200 µL volumes in a Greiner clear bottom 96 well plate (Greiner 655090). Cultures were grown for 20 h in a Spectramax i3x plate reader with medium plate shaking at 37 ºC with absorbance readings at 600 nm taken every 11 min to calculate doubling time and growth rate ...
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bioRxiv - Biochemistry 2022Quote: ... in RPMI 1640 5% FBS were seeded at a density of 4000 cells / well into 96 well flat bottom plates (Greiner, 655075) pre-coated with poly-L-lysine (Sigma P4707) ...
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bioRxiv - Microbiology 2023Quote: Assay plates were previously prepared with microbial extracts to be screened at a final concentration of 1/100 dilution of whole broth equivalent (WBE) and at a final DMSO concentration of 0.2% in 384 well black bottom-transparent plates (Greiner bio-one). MRC-5 infected cells were washed ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of the culture was transferred to 198 µL of fresh medium in a 96-microwell flat-bottom plate (Greiner, 655180) using an automated liquid handler ...
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bioRxiv - Bioengineering 2023Quote: ... single colonies were picked from agar plates for precultures (1.5 mL mineral media) in 14 mL round-bottom cultivation tube (Greiner Bio-One). Precultures were grown for 48 or 72 h (for slow growing Δpah1 strains ...
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bioRxiv - Biophysics 2023Quote: ... The cell suspension was incubated for 4 days with shaking using a Shel Lab incubator at 27 °C and 300 rpm in 24-deep well U-bottom plates covered with Breathe-Easy membranes (Greiner BioOne). A P0 viral stock was isolated by centrifugation at 2000 ×g for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... A total of 200 cells were seeded in 40 µL of N2B27 medium per well in a U-bottom low-adherence 96-well plate (Greiner CELLSTAR). After 48 h ...
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bioRxiv - Microbiology 2023Quote: We diluted allantoic fluid containing half-log serial dilutions of virus in Dulbecco’s PBS (DPBS) in a round-bottom 96-well plate (Greiner Bio-One). We combined 50 µl of the virus dilutions with 50 µl 0.5% turkey RBCs and incubated at four °C for one hour ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 µL of HepG2 cells at 2 × 105 cells/mL (1,000 cells/well) were dispensed into a 1536-well white solid-bottom tissue treated plate (Greiner Bio-One) using a Multidrop Combi dispenser (Thermo Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Turbidity measurements and fluorescence measurements were performed on a Tecan microplate reader GENios Plus in black 96-well plates with transparent flat bottom (Greiner, 655096) incubated at 37°C.