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Citations for Stemcell Technologies :
1 - 50 of 1298 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: We used the ALDEFLUOR detection kit (Stemcell Technologies; Cat#: 01700) to detect ALDH enzymatic activity in primary OSE cells ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Cancer Biology 2022Quote: ... After the incubation time ALDEFLUOR assay (Stem Cell Technologies, #01700) was performed as described previously (14).
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated at RT using SepMate PBMC Isolation Tubes (STEMCELL Technologies) by the UCSF Biospecimen Resource Program ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged 1-2 times per week with ReLeSR (STEMCELL Technologies) when they reached 80-90% confluence ...
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bioRxiv - Systems Biology 2022Quote: ... Organoids were washed three times with HBSS without Ca2+/Mg2+ (STEMCELL technologies, 37250). Single cell suspensions were obtained by adapting the protocol described in (Cowan et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were fed three times a week with mTeSR1 plus medium (STEMCELL Technologies) and passaged twice a week by dissociation in 0.02% EDTA ...
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bioRxiv - Developmental Biology 2023Quote: ... organoids were washed three times with HBSS without Ca2+ and Mg2+ (STEMCELL Technologies, 37250). Prewarmed papain solution (1-2 ml ...
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bioRxiv - Immunology 2021Quote: ... The pellet was washed twice each time with 20 mL of EasySep buffer (STEMCELL Technologies) followed by centrifugation at 400 rcf for 10 min with brakes on ...
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bioRxiv - Neuroscience 2023Quote: ... IPSCs were passaged 10 times before being transferred to the mTeSR1 culture system (StemCell Technologies, 85875).
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... Organoids were cut in halves and washed three times with HBSS without Ca2+/Mg2+ (STEMCELL technologies, 37250). Single cell suspensions were acquired by dissociation of the organoids with a papain-based dissociation (MACS Miltenyi Biotech 130-092-628) ...
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bioRxiv - Neuroscience 2023Quote: ... washed several times with sterile PBS and upon PBMCs were cultured in StemSpan SFEM medium (Stemcell Technologies) containing 2 mM Ultraglutamine (Lonza) ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
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bioRxiv - Developmental Biology 2023Quote: ... at which time cells were washed with PBS and incubated in ReleSR (Cat. # 05872, Stem Cell Technologies) for 1 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... differentiating cells were passaged for the first time by incubating them with Accutase (StemCell Technologies, #01-0006) for 18-30 minutes and reseeding them into Matrigel (BD Bioscience #354277)-coated 6-well plates at 1 x 105 cells/cm2 in cortical differentiation medium containing 10 µM Rock-inhibitor (Tocris ...
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bioRxiv - Cancer Biology 2023Quote: Patient PBMCs were collected at the time of surgery and isolated using SepMate-15 tubes (StemCell Technologies) and Lympholyte-H (Cedarlane ...
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bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
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bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed at minimum three times with filter-sterilized normal saline (0.9% NaCl) and trypsinized with 0.25% trypsin in citrate saline (STEMCELL Technologies, 07400). Cells were resuspended with DMEM with no phosphate (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: CFU assay was performed at the indicated time points plating 800 cells in methylcellulose-based medium (MethoCult H4434, StemCell Technologies) supplemented with penicillin and streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Splenocytes were isolated by shredding the spleen through a mesh followed by washing three times in ClonaCell-HY Medium B (StemCell technologies). Splenocytes were fused to SP2/0 cells using the ClonaCell-HY hybridoma Kit (StemCell cat ...
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bioRxiv - Physiology 2023Quote: ... Intestinal crypts were mechanically released from the lamina propria by vigorous pipetting and washed using PBS for twenty times followed by incubating in 25 mL gentle cell dissociation reagent (STEMCELL Technology) for 15min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Genetics 2023Quote: ... washed five times with water and prefilled with 100 µl per well of neural coating medium comprising Brainphys medium (STEMCELL Technologies) with 1× B27 plus vitamin A ...
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Cancer Biology 2021Quote: ... All GSC lines and human neural stem cells were maintained as neurospheres using ultra low-adherence culture dishes and dissociated 1-2 times per week with Accutase (StemCell Technologies 07922). Primary human astrocytes were cultured adherently on plates coated with Geltrex (Life Technologies A1413202) ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (Stemcell Technologies) was used to quantify the ALDH enzymatic activity ...
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bioRxiv - Neuroscience 2023Quote: ... StemDiff Hematopoietic Kit (Stemcell Technologies) was used to generated hematopoietic precursor cells (HPCs) ...
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bioRxiv - Immunology 2023Quote: ... Aldefluor kit (01700, Stemcell Technologies) was used for assessing RALDH activity ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ALDEFLUOR kit (StemCell technologies Cat #01700) was used for identification of cancer stem cells that express high levels of ALDH ...
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bioRxiv - Cancer Biology 2020Quote: The Aldefluor kit (Stem Cell Technologies) was used to characterize the ALDH activity of 4T1 cells as described in (Pearce et al ...
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bioRxiv - Molecular Biology 2021Quote: ... All kits are from STEMCELL Technologies, and differentiation was induced following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... The Aldefluor kit (Stem Cell Technologies) was used for the staining and quantification of ALDH1 activity by flow cytometry ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (StemCell Technologies, USA) was used to assess the population with a high ALDH enzymatic activity ...
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bioRxiv - Cancer Biology 2020Quote: ALDEFLUOR kit (STEMCELL Technologies, Cologne, Germany) was used to identify cells expressing high levels of ALDH enzyme ...
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bioRxiv - Neuroscience 2021Quote: ... STEMdiff Trilineage Differentiation Kit (StemCell Technologies) was used as described by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Neurocult Enzymatic Dissociation kit (Stemcell Technologies) was used for cell dissociation according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Selection kit II (STEMCELL Technologies, #17877) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: StemDiff T cell kit (StemCell Technologies) was used to differentiate HSPCs to lymphoid progenitor cells and to DP T cells ...
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bioRxiv - Neuroscience 2022Quote: ... At time of experimentation APCs were thawed and plated at 150,000 cell/cm2 in STEMdiff™ Astrocyte Maturation Media (Stem Cell Tech cat#100-0016) on Corning® Matrigel® coated tissue culture treated plate ...
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bioRxiv - Neuroscience 2023Quote: ... and frozen at −80°C for qRT-PCR or Neurosphere Basal Medium supplemented with Proliferation Supplement (STEMCELL Technologies) as described below ...