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Citations for Stemcell Technologies :
1 - 50 of 55 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Bioengineering 2023Quote: ... we initiated the intestinal organoid differentiation on the strips using a commercially available kit STEMdiff™ Intestinal Organoid Kit (05140, STEMCELL Technologies) containing the necessary medium for each differentiation stage ...
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bioRxiv - Cancer Biology 2020Quote: ... The epidermis was separated from the dermis by placing the strips in tubes containing 10 ml of 5U/ml Dispase II (Stem Cell Technologies, USA) and incubated at 4°C overnight and at 37°C for 2-3 hours ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... resuspended in EasySep Buffer(StemCell Technologies) and enriched using the EasySep FITC Positive Selection Kit II(StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... in CellAdhere dilution buffer (Stem Cell Technologies). Each coated plate was prepared fresh before use ...
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bioRxiv - Genomics 2021Quote: ... diluted in CellAdhere Buffer (Stem Cell Technologies, Catalog Number ...
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bioRxiv - Cell Biology 2023Quote: ... and washed with EasySep buffer (STEMCELL Technologies) at 400 g for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 9 μL Buffer R2 (STEMCELL Technologies, 100-0691), 1 μL 30 μM of gRNA (Table 2) ...
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bioRxiv - Immunology 2023Quote: ... The tissue solution was diluted in RoboSep buffer (Stemcell Technologies) and then passed through a 100µm cell strainer into a 50mL tube ...
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bioRxiv - Bioengineering 2020Quote: ... Blood was then washed with RBC Lysis Buffer (Stem Cell Technologies), then centrifuged at 16 000 g for 10 min ...
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bioRxiv - Immunology 2021Quote: ... EasySep Buffer (Cat. No. 19762, 19666, and 20144 from STEMCELL Technologies), and Calcein AM (Cat no ...
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bioRxiv - Immunology 2020Quote: ... Cells were then placed in Robosep buffer (Stemcell technologies, Vancouver, BC) at 23-50 million/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were then treated with Gentle dissociation buffer (Stem Cell Technologies) and placed on an orbital rotator for 15 minutes @ 50rpm (room temp) ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 μL red blood cell lysis buffer (StemCell Technologies) with a wide-bore P1000 tip ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with 1:10 Hank’s buffer (STEMCELL Technologies 37150) supplemented with 10 µM Y-27632 (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... cells were exposed to red blood cell lysis buffer (STEMCELL Technologies) for 2 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Cultures were expanded weekly with Gentle Cell Dissociation Buffer (GCDR, Stem Cell Technologies) at 70-90% confluency at a 1:10 ratio ...
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bioRxiv - Immunology 2022Quote: ... and red blood cells were removed using the RBC lysis buffer (StemCell Technologies). Mononuclear cells were washed three times with PBS and CD34+ cells were isolated as described 35 using rhesus macaque-specific antibodies to CD34 (clone 563 ...
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bioRxiv - Microbiology 2021Quote: ... About 2×105 cells were washed twice in Robosep buffer (#20104, Stemcell Technologies) and fixed in 300 μl CellFix buffer 1X (#340181 ...
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bioRxiv - Cancer Biology 2019Quote: Single dissociated cells were suspended in Aldefluor assay buffer (STEMCELL technologies, Cambridge UK) and incubated with an ALDH substrate ...
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bioRxiv - Immunology 2021Quote: ... Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies) containing following phenotyping antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... and then incubated in a digestion buffer of 5 Units/mL dispase (Stemcell Technologies, #07913) and 0.1 mg/mL DNase I (Worthington ...
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bioRxiv - Immunology 2021Quote: ... The pellet was washed twice each time with 20 mL of EasySep buffer (STEMCELL Technologies) followed by centrifugation at 400 rcf for 10 min with brakes on ...
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bioRxiv - Cancer Biology 2022Quote: Bone marrow cells were subjected to RBC lysis using Ammonium Chloride lysis buffer (Stem Cell Technologies), then incubated with anti-mouse direct lineage depletion cocktail (Miltenyi ...
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bioRxiv - Neuroscience 2020Quote: ... iPSC were grown to near confluence in E8 media before detaching colonies in gentle dissociation buffer (StemCell Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting sample was resuspended to a concentration of 50 million cells/mL in EasySep Buffer (STEMCELL Technologies). 50 μL/mL isolation cocktail and 50 μL/mL cocktail enhancer were added to the sample ...
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bioRxiv - Cancer Biology 2021Quote: ... Sigma for 2 min at room temperature and then incubated in neutralization buffer supplemented with NH4Cl (0.8%, StemCell Technologies) to remove red blood cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellet was resuspended into HF buffer (HBSS, 2% fetal bovine serum, 10mM Hepes pH 7.2) and NH4Cl (StemCell Technologies). The cell pellet was recovered after 5 min of centrifugation at 1000rpm and resuspended in organoid medium for organoid culture ...
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bioRxiv - Cancer Biology 2020Quote: ... Skin was disinfected with 10% iodine in phosphate saline buffer (PBS) and incubated with 2U/ml of dispase (STEMCELL technologies) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: NSTcPA buffer (1 mL) was pipetted into one well of a 6-well plate (Stem Cell Technologies, cat. no. 38015) on ice ...
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bioRxiv - Genomics 2022Quote: ... An addition 1.65 mL of homogenization buffer was topped up and mixed with 2.65 mL of gradient medium (5mM CaCl2, 50% Optiprep (Stemcell Technologies), 3mM Magnesium Acetate ...
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bioRxiv - Cell Biology 2021Quote: Hepatostellate organoids at day 17 were treated with dissociation buffer, (1:1:1 mixture of 0.25% Trypsin-EDTA, Accutase (Stem cell Tech.), and Collagenase IV (1 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting single cell suspension was than washed with 10 mL of cold RPMI and resuspended in 1 mL of Robosep buffer (STEMCELL technologies) prior to the negative selection of B- or T-cells ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... Briefly, PB was collected in FACS buffer (PBS, 2 % FBS, 2 mM EDTA) supplemented with 0.0004 % heparin solution (STEMCELL Technologies Inc.). RBCs was removed by incubation at 37 °C for 25 minutes with 1 % Dextran solution in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... were prepared using the osmotic shock protocol and burst onto freshly plasma-cleaned Si/SiO2 chips containing 5 µm diameter holes in presence of HEPES buffer (25 mM HEPES, 140 mM KCl, 1 mM DTT) supplemented with index matching 45% Optiprep(TM) (Stemcell Technologies) and 5 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... was prepared using the osmotic shock protocol and burst onto freshly plasma-cleaned Si/SiO2 chips containing 5 µm diameter holes in presence of HEPES buffer (25 mM HEPES, 140 mM KCl, 1 mM DTT) supplemented with index matching 45% Optiprep(TM) (Stemcell Technologies) and 5 mM MgCl2 70 ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... To confirm Treg depletion spleen and lymph nodes were mashed through a 70 μm strainer and then treated with red blood cell lysis buffer (STEMCELL Technologies) for 2 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Organoids were cut into small pieces using a scalpel and thoroughly washed with HBSS buffer without Ca2+/Mg2+ (StemCell Technologies, #37250) to remove debris.
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bioRxiv - Cell Biology 2024Quote: ... 1mM EDTA buffer and monocytes were then isolated using the magnetic EasySep Human CD14+ Selection kit (STEMCELL Technologies, Vancouver, BC, Canada) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells were removed from single cell suspension using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... blood samples were diluted with an equal amount of phosphate buffer saline solution (PBS) and slowly layered on top of the same volume of Lymphoprep density gradient medium (Stemcell Technologies Inc.). After that ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...