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Citations for Stemcell Technologies :
501 - 550 of 2339 citations for Thyroxine T4 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
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bioRxiv - Cell Biology 2023Quote: Luminal or luminal progenitor cells were isolated from mouse mammary glands and sorted in 96-well plate filled with 5% FBS EpiCULT full medium (STEMCELL Technologies). 500 luminal cells or 200 luminal progenitors were then transferred to a 48-well plate previously seeded with 10,000 NIH3T3 irradiated feeder cells per well and incubated at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Developmental Biology 2024Quote: ... A 1-5 min centrifugation of the well-plate was performed at 200g and cells in suspension were cultured for one day in mTesR Plus (StemCell Technologies) to allow for aggregation and Embryoid Body (EB ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured for 72 hours in 96-well plates (2X105-5X105 cells per well) containing StemPro (Stem Cell Technologies) media supplemented with human IL-6 ...
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bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
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bioRxiv - Immunology 2020Quote: ... the EasySep Kit (StemCell Technologies, cat. 18000) was used according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... NK-cell Enrichment Kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the ALDEFLUOR kit was used (StemCell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... using memory CD8+ isolation kit (StemCell Technologies). T cells were harvested and washed three times with Opti-MEM (LifeTechnologies) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... STEMdiff Cerebral Organoid Kit (StemCell Technologies, 08570) was used for organoid culture ...
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bioRxiv - Immunology 2022Quote: ... and then the ALDEFLUOR kit (Stemcell Technologies) protocol was followed ...
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bioRxiv - Developmental Biology 2023Quote: ... StemLight Pluripotency Antibody Kit (Stem Cell Technologies) was used for pluripotency testing ...
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bioRxiv - Developmental Biology 2023Quote: ... The hPSC Genetic Analysis Kit (Stemcell Technologies) was used following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and c-Kit/CD117 antibodies (STEMCELL Technologies) for 30 min ...
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bioRxiv - Cell Biology 2019Quote: Whole-brain cerebral organoids were generated with a commercial kit (STEMdiff Cerebral Organoid Kit, STEMCELL Technologies, Catalogue Number 08570), which has been adopted and optimized based on previously published protocol50 ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Cell Biology 2020Quote: ... cells in a 6 well plate were washed with 1X PBS then subjected to 2.5 units/ml of dispase (Stemcell Technologies, Kent, WA). The plate was placed on an orbital shaker to induce mechanical stress at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Two conventional and established conditions were used as indicated throughout the figures and manuscript: TeSR conditions on Matrigel coated plates (Stem Cell Technologies) or FGF/KSR conditions on MEF substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... were a gift from the Nik-Zainal lab (University of Cambridge, UK) and cultured on non-tissue culture treated plates (Stem Cell Technologies) pre-coated with 10 μg/mL Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... the media was aspirated from the transwell which was then transported to a new 24-well plate and 600 μL of PneumaCult™ALI Maintenance medium (STEMCELL Technologies) was added to the basolateral side ...
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bioRxiv - Immunology 2022Quote: ... The transwells were then moved to a new 24-well plate wherein each test well contained 600 μL of fresh PneumaCult™-ALI Complete Base Medium (STEMCELL Technologies) for the basolateral side ...
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bioRxiv - Developmental Biology 2019Quote: ... all the cells transfected with Sendai virus were transferred into a 6-well plate (30,000 cells per well) each well containing ~150,000 mouse embryonic fibroblast (MEF) feeder cells (Stemcell Technologies Inc. Vancouver, Canada). The MEF plate preparation was conducted the day before plating the reprogramming cells as explained elsewhere (28) ...
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bioRxiv - Bioengineering 2020Quote: ... to sort single cells into Matrigel-coated 96-well plates with each well containing 150 μl of mTeSR1 supplemented with CloneR (STEMCELL Technologies, #05888). Following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... were cultured in clear 96-well Greiner COC plates which were plasma treated and coated with 0.1% gelatin (STEMCELL Technologies, Vancouver, Canada). iCell cardiomyocytes plating medium from the iCell Cardiomyocytes Media Kit was used to plate the cardiomyocytes at 60,000 cells/well and then exchanged with iCell Cardiomyocytes maintenance medium after 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
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bioRxiv - Cancer Biology 2019Quote: Patient-derived PB and/or BM MNC were plated at 4 x 104 cells per well in 96-well plates in 200 µL of RPMI supplemented with 10% fetal bovine serum and 10X StemSpan (StemCell Technologies #02691) (Pardanani et al. ...
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bioRxiv - Cancer Biology 2019Quote: Single cells were FACS-isolated into flat bottomed 96-well plates containing 100 μl of MethoCultTM H4435 Enriched (StemCell Technologies Cat#04435). Colonies were visually inspected and classified 11-14 days after plating ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... 288 mio NPCs per axis were aggregated into 14400 organoids (20000 cells/per organoid) in Aggrewell800 6-well plates (Stem Cell Technologies). The day after ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genomics 2021Quote: ... The sorted cells were subsequently used as input for a second sort in which single cells were deposited into 384-well plate wells containing 20μl StemSpan™ SFEM II (Stem Cell Technologies) with 1X CD34+ Expansion Supplement (Stem Cell Technologies) ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Developmental Biology 2022Quote: KOLF2 iPS cells were grown under feeder-free conditions on Matrigel-coated plates in TeSR™-E8™ (Stem Cell Technologies, #05990).
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bioRxiv - Synthetic Biology 2022Quote: Human iPSC line PGPC-17/11 (Personal Genome Project Canada) was cultured on Matrigel-coated plates in mTeSR Plus medium (STEMCELL Technologies 05825) and passaged with ReLeSR (STEMCELL Technologies 05873 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated into 96 well flat bottom plates in growth medium supplemented with 10 µM of ROCK inhibitor (Stem Cell Technologies). Cells were plated at low density (2,500 cells/well ...
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and 2.8×105 cells/cm2 were plated on Geltrex-coated plates in mTesR+ supplemented with ROCK inhibitor Y-27632 (10uM, StemCell Technologies, 72302). Neuronal induction was started 24h later (day 0 ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... after expansion the cells were prepared for embryoid body formation using the AggreWell™ EB 400 microwell plate following the manufacturers recommendations (StemCell Technologies). Cells were dissociated in Accutase (Innovative Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 5.8 × 105 cells in N2/B27 media and supplements were seeded into one well of a 6 well AggreWell™400 culture plate (STEMCELL Technologies) which was coated with 5% Pluronic acid F-127 (Sigma-Aldrich P2443 ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).