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Citations for Stemcell Technologies :
51 - 100 of 970 citations for TNFRSF10C Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Immunology 2019Quote: Primary human T cells were isolated from healthy donors using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies). Buffy coats were purchased from Stanford Blood Center and processed according to the manufacturer’s protocol using Lymphoprep density gradient medium and SepMate-50 tubes ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Purified human neutrophils were extracted from whole blood using the EasySep™ Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, BC) and performed in accordance with the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: Human cord-blood CD34+ cells were isolated by magnetic sorting using EasySep human CD34 positive selection kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
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bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
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bioRxiv - Immunology 2022Quote: ... recombinant human FGF (25ug/mL; Stem Cell Technologies), and recombinant human EGF (10 ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Human Recombinant IFNγ (10ng/mL, StemCell Technologies 78020) was added to the media for a period of 60 minutes ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... or anti-human CD32 (FcγRII Blocker, StemCell Technologies) for 15 minutes on ice to block non-specific staining and then incubated with fluorochrome-conjugated antibodies for 30 minutes on ice and protected from light ...
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bioRxiv - Microbiology 2020Quote: ... and RosetteSep Human CD8 Depletion Cocktail (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant EGF (20 ng/mL, human, STEMCELL Technologies) and antibiotic antimycotic solution (100x ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies, and dead PBMCs were prepared by treating them with 70% ethanol for 30 min ...
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bioRxiv - Immunology 2023Quote: ... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
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bioRxiv - Biochemistry 2023Quote: Human peripheral blood Pan-T cells (Stemcell Technologies) were thawed and expanded following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human Peripheral Blood Plasma (3%; Stem Cell Technologies), 3,3‘,5-Triiodo-L-thyronine (1 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Noggin (100 ng/ml, Stemcell Technologies), B-27 supplement (ThermoFisher) ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant bFGF (20 ng/mL, human, STEMCELL Technologies), recombinant EGF (20 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) was used to enrich for CD34+ cells to >90% purity.
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bioRxiv - Immunology 2023Quote: ... and EasySep Human Monocyte Isolation Kit (StemCell Technologies). In the coculture experiment ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies. After thawing by immediately placing them into a 37°C water bath and transferring them into a pre-warmed RPMI1640 with 10% FBS and 1% Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... human recombinant bFGF (hrbFGF) (Stem Cell Technologies, 78003), and heparin (Stem Cell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Human Intesticult™ (StemCell Technologies, Cambridge, MA, USA) with Primocin® (20 µl of a 500X stock ...
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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bioRxiv - Microbiology 2020Quote: A lentiviral vector encoding anti-HIV-1 CAR was transduced and expressed in the enriched total human T cells obtained from fresh peripheral blood mononuclear cells by negative selection (EasySep Human T cell isolation kit, Stemcell Technologies). CAR-T cells were maintained in IMDM supplemented with 20% FBS ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood NK cells were isolated from blood collars using a RosetteSep™ human NK cell enrichment cocktail (STEMCELL Technologies). NK cells were then co-cultured for 4 h with confluent monolayers of SH-SY5Y parental ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Microbiology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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bioRxiv - Cancer Biology 2021Quote: Aliquots of fresh blood or frozen ficoll-separated peripheral blood cells were fractionated using EasySep Human Myeloid Positive Selection and EasySep Human T Cell Enrichment kits (StemCell Technologies. DNA was extracted from the fractionated subsets using MagMAX DNA Multi-Sample Ultra 2.0 Kit (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... were isolated from perioperative human patients’ brain tissues (Nervous System Tumor Bank) using EasySep Human CD8 T Cells Isolation Kit (STEMCELL Technologies) following the tissue digestion as previously described [68 ...
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bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs) purified from PBMCs by negative selection with the EasySep Human Plasmacytoid DC enrichment kit (STEMCELL Technologies). pDCs were cultured in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Human naïve T cells were isolated from PBMCs using the EasySep™ Human naïve CD4+ T Cell Isolation Kit Human (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells and CD25hi CD4+ T cells were negatively isolated from PBMCs separately by using Easysep human CD4+ T cell isolation kits and EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Further T cells isolation from human PBMCs was done with EasySep™ Human T Cell Isolation Kit (STEMCELL Technologies, 100-0695) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... Primary human CD3+ T cells were isolated from PBMCs by negative selection using EasySep Human T Cell Isolation Kit (StemCell Technologies) or by positive selection using Dynabeads Human T-Expander CD3/CD28 (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: Human CD8+ T cells were obtained from either pooled PBMC donors (ZenBio) using negative selection human CD8 isolation kits (StemCell Technologies) or directly from vials containing isolated CD8+ T cells from individual donors (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were generated from healthy human donors by differentiation of CD14+ monocytes isolated using a RosetteSep human monocyte enrichment kit (STEMCELL Technologies). In order to generate MDSCs ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated by negative selection using the EasySep Human CD8 negative isolation kit (StemCell Technologies, Vancouver, BC).
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ and CD8+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ or CD8+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...