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Citations for Stemcell Technologies :
451 - 500 of 975 citations for TIGIT Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies, 17955) and activated by 100 Gy irradiated K562 Clone 9 feeder cells ...
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bioRxiv - Developmental Biology 2021Quote: Human ESCs were differentiated into neural progenitor cells (NPCs) using the STEMdiff neural system (STEMCELL Technologies). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc.). The resulting CD34+ HSPCs were cultured in HSPC media ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line (WA01, WiCell) was maintained with mTeSR plus medium (Stemcell Technologies) on 6 well plates coated with Matrigel (Corning) ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpan™ medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: ... Pure cell populations were isolated using EasySep Human negative selection kits (STEM CELL technologies, >95% purity). In addition ...
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bioRxiv - Systems Biology 2021Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated using the Direct Human PBMC Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol and washed/resuspended in custom RPMI-1640 deficient in biotin ...
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bioRxiv - Cancer Biology 2020Quote: ... Red blood cell lysis and leukocyte depletion (EasySep Human CD45 Depletion Kit II #17898, STEMCELL Technologies) of MPE were performed before processing with 10X Genomics ...
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bioRxiv - Cancer Biology 2020Quote: ... Mesenchymal: Adipocyte differentiation was carried out using the MesenCult Human Adipogenic Differentiation kit (STEMCELL Technologies #05412) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Primary human T cells were isolated by negative selection for CD3+ populations (STEMCELL technologies, Easy Sep) from anonymous healthy human donor blood collected by leukopheresis and purchased from STEMCELL technologies ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II (Stemcell Technologies, #17877) for analysis in a companion study (manuscript in preparation) ...
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bioRxiv - Neuroscience 2022Quote: Cerebral organoids were differentiated from human iPSCs and cultured with STEMdiff Cerebral Organoid Kit (STEMCELL Technologies) according to the manufacture’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were isolated with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Genetics 2022Quote: ... Human KOLF_2 (HIPSCI, www.hipsci.org) were grown in feeder-free conditions in TeSR-E8 medium (StemCell Technologies) on Synthemax (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... Human enteroid differentiation was initiated by replacing expansion media with differentiation (Intesticult Component A (StemCell Technologies) mixed at a 1:1 ratio with DMEM/F12 supplemented with 15mM HEPES ...
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bioRxiv - Immunology 2019Quote: ... Bulk T cells were isolated from PBMCs using Easysep Human T Cell Isolation Kit (STEMCELL Technologies) and activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... 25 µL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies - #10970) were used ...
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bioRxiv - Physiology 2022Quote: ... Human iPSCs were grown in mTeSR1 basal medium supplemented with mTeSR1 5x supplement (StemCell Technologies, UK) and 100 U/mL penicillin/streptomycin on cell culture plates coated with DMEM/F12 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Half well equivalent was seeded into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Immunology 2019Quote: ... Human Cord Blood (CB) purified CD34+ cells from 5 different donors were obtained from STEMCELL Technologies and resuspended in StemSpan Expansion Media – SFEM II (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... cells in full media were incubated with Human Recombinant IFNα-2A (10ng/mL, StemCell Technologies 78076.1) or IFNγ (10ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: Unencapsulated 50 cell aggregates were created by dissociation of human iPSCs with Accutase (STEM CELL Technologies) and reseeding in mTeSR™-1 medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: Human T-cell subsets were isolated from leukapheresis produts using negative selection kits from STEMCELL Technologies. Rosette Sep (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpanTM medium (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... feeder-free maintenance medium (mTeSR for human ES and iPS cells, Stem Cell Technologies, cat#85850) as previously described (Low et al. ...
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bioRxiv - Genomics 2019Quote: ... H1 human ESCs (WA01, WiCell Research Institute) were maintained in feeder-free mTesR1 medium (Stemcell Technologies). hESCs (passage 26 ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Genomics 2019Quote: ... neutrophils were further purified to homogeneity using an EasySep Human Neutrophil Enrichment kit (Stemcell technologies, 19257) as per the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and T cells isolated using the EasySep Human T Cell Isolation kit (Stemcell Technologies, #17951). Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
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bioRxiv - Neuroscience 2022Quote: ... human neuron-astrocyte co-cultures were maintained in neuron maintenance medium (1X BrainPhys Basal (StemCell Technology), 1X B27 with Vitamin A (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSCs (IMR90-4; WiCell Research Institute) were maintained with mTeSR Plus (STEMCELL Technologies, 100-0276) on dishes coated with 9-10 μg/cm2 Growth Factor Reduced Matrigel (Corning ...
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bioRxiv - Immunology 2022Quote: ... NK cells isolated from buffy coats using an EasySep human NK cell enrichment kit (StemCell Technologies) were added to the mixed target population at a 1:1 effector:target ratio ...
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bioRxiv - Immunology 2022Quote: ... NGFR+ cells were bead-purified by an EasySep Human CD271 Positive Selection Kit II (STEMCELL Technologies). See Fig ...
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bioRxiv - Immunology 2022Quote: ... NK cells were enriched from PBMCs by EasySep™ Human NK Cell Isolation Kit (StemCell Technologies) and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: Frozen human bone marrow mononuclear cells (BMMC) from three healthy donors were purchased from STEMCELL Technologies and stored in liquid nitrogen until use ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were purified by negative selection using RosetteSepTM Human T cell enrichment cocktail (STEMCELL Technologies) following manufacturer’s instructions ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... CD34+ HSPC were positively selected using the EasySepTM Human CD34 Positive Selection Kit II (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... to collect mononuclear cells then purified using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). HUCB CD34+ cells were then seeded in Matrigel Matrix (Cat# 356231 ...
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bioRxiv - Microbiology 2023Quote: ... primary human monocyte derived macrophages were detached from 12-well plate by using accutase (Stemcell Technologies). Digested macrophages were washed with PBS for 2 times in 96-well plates and fixed in fixation buffer (1% paraformaldehyde (PFA) ...
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bioRxiv - Bioengineering 2023Quote: Magnetic sorting for CD19 using the EasySep human CD19 positive selection kit II (StemCell Technologies Inc.) involved the same dissociation procedure as above ...
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bioRxiv - Cell Biology 2023Quote: ... All human CD34+ HSPCs were cultured in StemSpan SFEM II medium (STEMCELL Technologies, Cambridge, MA, USA) supplemented with stem cell factor (SCF ...
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bioRxiv - Cell Biology 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained on vitronectin XFTM (10 μg/mL, StemCell Technologies)-coated plates and in Essential 8 (E8 ...