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Citations for Stemcell Technologies :
501 - 550 of 4274 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were cultured in collagen-I gels in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, Cat# 10981) with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... Isolation of T and B cells were performed according to the manufacturer’s protocols (Stemcell Technologies).
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bioRxiv - Immunology 2021Quote: ... we respectively used mouse- and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Bioengineering 2020Quote: Human T cells were obtained by purchasing whole blood units from STEMCELL technologies (Vancouver, Canada). STEMCELL reported that these products are distributed using Institutional Review Board (IRB)-approved consent forms and protocols ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... Primary CD4+ and CD8+ T cells were isolated by negative selection (STEMCELL Technologies #15062 & 15063). CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130 ...
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bioRxiv - Bioengineering 2021Quote: ... were expanded using fresh complete ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies - #10981) additionally supplemented with cytokines (Human Recombinant IL-2 ...
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bioRxiv - Immunology 2020Quote: ... enrichment of CD8+ T cells was performed using magnetic bead separation kits EasySep (STEMCELL Technologies) or Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated using the CD4+ Negative Selection Kit (StemCell Technologies, Cat# 19752) followed by fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2021Quote: ... CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies, Vancouver, Canada) per manufacturer’s instructions and confirmed >90% pure by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated from PBMCs using the EasySep positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Tregs and Tconvs were cultured in ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) supplemented with 50 μmol/L of β- mercaptoethanol and 100 units/mL of Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... we used mouse-specific and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Neuroscience 2023Quote: ... CD4+ T cells were immunomagnetically purified by negative selection according to manufacturer’s instructions (STEMCELL Technologies). Isolated CD4+ T cells were then subjected to a CD25+ cell isolation (STEMCELl Technologies ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with 25μL/mL ImmunoCult Human αCD2/αCD3/αCD28 T cell Activator (STEMCELL Technologies) or 10μL/mL T-cell TransAct αCD3/αCD28 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... magnetically isolated splenic P14 CD45.1+ TN (EasySep Mouse CD8+ T cell isolation kit, STEMCELL Technologies) were labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Immunology 2023Quote: ... 2D2 and OT-I cells were isolated respectively with magnetic CD4+ and CD8+ T cell selection beads (EasySep, STEMCELL Technologies).
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... media was removed from the apical surface and cells were fed basolaterally with Pneuma-Cult ALI (Air Liquid Interface) differentiation medium (STEMCELL TECHNOLOGIES) for 5-8 weeks to allow differentiation of cells at the air-liquid interface ...
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bioRxiv - Immunology 2022Quote: ... and 30 μl of additional biotinylated anti-CD3 (STEMCELL Technologies, 18051) was added to the sample to improve purity ...
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bioRxiv - Immunology 2019Quote: ... and the EasySep Biotin Positive Selection Kit (Stemcell Technologies) according to product instructions ...
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bioRxiv - Immunology 2021Quote: ... the EasySep Biotin Positive Selection Kit II (Stemcell Technologies), and biotinylated anti-mouse CD49b antibody (25 µg/ 1×108 via.cells ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Viable T cells were enriched using the Easy Sep T cell isolation Kit followed by the Easy Sep Dead Cell removal Kit (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Monocytes were enriched from the same leukopoiesis products as T-cells using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4 ...
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bioRxiv - Cancer Biology 2019Quote: ... CD4+ or CD8+ T cell enrichment was performed using magnetic beads (Miltenyi Biotec or STEMCELL Technologies). Naïve CD4+ T cells were isolated using a naive CD4+ T cell isolation kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: from CD4-T cells following the protocol described in the EasySep PE-selection kit (STEMCELL technologies). Cells from the negative fraction (CD4+CD25- ...
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bioRxiv - Immunology 2019Quote: Human T-cell subsets were isolated from leukapheresis produts using negative selection kits from STEMCELL Technologies. Rosette Sep (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Immuno-magnetic T and B cell depletion was performed using the EasySep magnetic system (StemCell Technologies), prior to setting up in vitro cultures ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... responder CD4+ T cells were isolated from OTII BL/6 mice by negative selection (STEMCELL Technologies). Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... ImmunoCult-XF T Cell Expansion Medium and immunomagnetic negative selection kits were purchased from STEMCELL Technologies. FITC-dextran molecules (500 kDa ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Genomics 2021Quote: ... CD8+ T cells were purified from thawed PBMCs by negative selection using EasySep Human CD8+ T Cell Enrichment Kit (Cat#19053, STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Aliquots of fresh blood or frozen ficoll-separated peripheral blood cells were fractionated using EasySep Human Myeloid Positive Selection and EasySep Human T Cell Enrichment kits (StemCell Technologies. DNA was extracted from the fractionated subsets using MagMAX DNA Multi-Sample Ultra 2.0 Kit (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...