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Citations for Stemcell Technologies :
1 - 50 of 68 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 5) DMEM/F-12 with 15 mM HEPES (DMEM/F-12, STEMCELL Technologies, 36254) and 1% w/v Bovine Serum Albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... LDN193189 (100 nM; D1-12; STEMCELL Technologies) and SB431542 (10 µM ...
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bioRxiv - Cell Biology 2024Quote: ... and the remaining cells were collected into 96-well plates (1 cell per well) containing Dulbecco’s modified Eagle’s medium F-12 (DMEM/F-12) with MesenCult™-SF attachment substrate (StemCell Technologies) and 1% antibiotic-antimycotic (Life Technologies) ...
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bioRxiv - Genomics 2021Quote: ... diluted in DMEM/F-12 (#36254, Stemcell Technologies) at 37°C for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: Fresh human intestinal samples were collected and kept in DMEM/F-12 with 15 mM HEPES (DMEM/F-12, STEMCELL Technologies, 36254) and 1% w/v Bovine Serum Albumin (BSA ...
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bioRxiv - Bioengineering 2023Quote: ... cells are sequentially treated with CHIR99021 (12 μM) (Stemcell Technologies, #72052) for 24 hours followed by RPMI 1640 medium with B-27 supplement without insulin (2% ...
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bioRxiv - Bioengineering 2024Quote: ... diluted in DMEM-F/12 (Stem Cell Technologies cat. no. 36254) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 12-well plate coated with vitronectin (STEMCELL Technologies, Inc.). Stemspan medium was replaced with MTesR complete medium following 72 h transduction ...
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bioRxiv - Neuroscience 2024Quote: ... coated 12 well plates in NM supplemented with 10 ug/mL BDNF (StemCell Technologies, #78005). Neurons were observed after 24-72 h ...
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bioRxiv - Cell Biology 2023Quote: ... and rosettes were selected after 12 days and expanded in Neural Progenitor Medium (StemCell Technologies). NPCs were differentiated to astrocytes using the Stem Cell Technologies STEMdiff astrocyte differentiation and maturation kits during which they were split weekly with Accutase (Millipore).
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bioRxiv - Bioengineering 2024Quote: ... Cells were routinely subcultured at a 1:12 ratio using ReLeSR Passaging Reagent (STEMCELL Technologies) every four days or at 70-80% confluency ...
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bioRxiv - Microbiology 2023Quote: ... primary human monocyte derived macrophages were detached from 12-well plate by using accutase (Stemcell Technologies). Digested macrophages were washed with PBS for 2 times in 96-well plates and fixed in fixation buffer (1% paraformaldehyde (PFA) ...
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bioRxiv - Cell Biology 2024Quote: ... cells and beads were resuspended in complete medium with 12 or 20% OptiPrep (STEMCELL Technologies, UK) to limit object sedimentation during loading ...
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bioRxiv - Cell Biology 2022Quote: ... and collected in medium containing advanced DMEM/F-12 with 15 mM HEPES (STEM CELL Technologies) supplemented with 100 μg/ml Penicillin-Streptomycin ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were cultured in 12-well plate coated with anti-adherence rinsing solution (STEM CELL TECHNOLOGIES).
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bioRxiv - Bioengineering 2023Quote: ... on Matrigel-coated 6-well plate and passaged 1:12 with ReLeSR™ (Catalog # 05872, STEMCELL Technologies) every four days.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were cultured onto Matrigel-coated 12-well plate in mTeSR1 Plus medium (STEMCELL Technologies, Vancouver, Canada) with 10 μM Y27632 (ROCKi ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were differentiated for 12 days using the STEMdiff™ Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.) [43] ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were seeded on Geltrex-coated 12-well plates in STEMdiff Neural Progenitor Medium (STEMCELL Technologies) supplemented with 10 nM Y-27632 ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-lymphocytes were supplemented with the following: TH1: 15 ng/mL IL-12 (StemCell Technologies #78028.1), 150 ng/µLIL-2 (StemCell Technologies #78081) ...
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Genomics 2021Quote: ... tissues used (15 CKD,12 AKI and 18 LD biopsy cores) were preserved using CryoStor® (Stemcell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... and plated at ∼40 colonies per well into Matrigel-coated 12-well plates with mTesR media (StemCell Technologies) containing ROCK inhibitor Y-27632 (10 uM ...
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bioRxiv - Developmental Biology 2023Quote: ... ENR medium is composed of advanced DMEM/F-12 with 15mM HEPES (Catalog no. 36254, Stem Cell Technologies) supplemented with 100 μg/ml Pen/Strep (Catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Activin-A (10 ng/ml) and 12 ng/ml FGF2 on 10μg/ml Vitronectin-XF (STEMCELL Technologies) or 0.1% Gelatin and MEF-medium coated plates ...
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bioRxiv - Genomics 2022Quote: ... and quenched with DMEM/F-12 GlutaMAX supplemented with 10 µM of ROCK inhibitor (Y-27632, StemCell Technologies, #72304) and 0.033 mg/ml of DNase (DNase (D2) ...
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bioRxiv - Cell Biology 2022Quote: ... The collected cells were resuspended in ENR medium (advanced DMEM/F-12 with 15 mM HEPES (STEM CELL Technologies) supplemented with 100 μg/ml Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... 12 ng/mL human-bFGF (#78006) and 2 ug/mL heparin solution 0.2% (#07980) from Stemcell Technologies (Vancouver, Canada). Neurospheres were dissociated using Accutase (#00-4555-56 ...
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bioRxiv - Cancer Biology 2024Quote: ... isolated glands were minced and digested for 16-20 hr at 37 °C in DMEM F-12 (StemCell Technologies, 36254) containing 2% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies) and cultured for 7 days with daily medium exchange ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated into single-cell suspension using Accutase and re-seeded onto fresh Poly-L-Ornithine / Laminin coated 12-well plates at a density of 150,000 cells in STEMdiff Neuron Maturation Kit medium (Stemcell Technologies) and medium exchanged every other day until days 14 and 21.
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bioRxiv - Cancer Biology 2023Quote: ... Single HSCs (Lin-cKIT+ Sca1+ CD48-CD150+) were FACS-isolated and expanded for 12 days in StemSpan SFEM (Stemcell Technologies) containing 1% Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... and plated at 40 colonies/well on Matrigel-coated (0.08 mg/well) 12-well plates in mTeSR1 media (StemCell Technologies) supplemented with mTeSR1 5× Supplement (StemCell Technologies) ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured throughout on Matrigel substrates in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 with 15mM HEPES (DMEM/F12, STEMCELL Technologies, 36254) 1X Insulin Transferrin Selenium Liquid Media Supplement (ITS ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were cultured throughout on Matrigel substrates in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 with 15mM HEPES (DMEM/F12, STEMCELL Technologies, 36254) 1X Insulin Transferrin Selenium Liquid Media Supplement (ITS ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Cell Biology 2020Quote: Serum-free conditioned medium (CM) was essentially generated as described (8, 12, 13) by incubating serum-free medium (BIT 9500, STEMCELL Technologies) for three days on irradiated (30 Gy ...
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bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Genomics 2023Quote: ... human iPSCs were plated at cell density of 3.5×105 cells/well on Matrigel coated 12-well-plates using mTeSR™ medium supplemented with 5µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stimulated with 12 μL/mL ImmunoCult™(TM) Human CD3/CD28 T Cell Activator (STEMCELL Technologies, cat. #10971).
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then seeded (1 × 104 cell/cm2) on rat tail collagen type 1-coated permeable Transwell membranes (12 mm; no. 38023, StemCell Technologies) until confluent ...
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bioRxiv - Immunology 2022Quote: ... 07180)-coated tissue culture plates and split in a ratio of 1:6 to 1:12 every four to six days with Gentle Cell Dissociation Reagent (STEMCELL Technologies, 07174). Alveolar differentiation was induced as previously described (Riva et al. ...
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bioRxiv - Neuroscience 2022Quote: ... five to seven day old iPSCs cultures were then used to generate cluster of differentiating 43 positive (CD43+) hematopoietic progenitor cells (HPCs) following a 12 day commercially available kit (STEMdiff Hematopoietic Kit; Stemcell Technologies # 05310). IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923 ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...