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Citations for Stemcell Technologies :
501 - 550 of 970 citations for Serpin A3 Human K267R HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Human mesoderm cells were derived from hESCs using the STEMdiff mesoderm induction medium (Stem Cell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic stem cells (hESCs; H1) were maintained in mTeSR1 plus media (StemCell Technologies, 100-0276) on Matrigel (Corning ...
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bioRxiv - Immunology 2024Quote: Human peripheral blood leukopaks from de-identified healthy donors were purchased from STEMCELL Technologies (Vancouver, Canada). CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Human fetal tissue derived U5 NCSs were cultured in NeuroCult NS-A basal medium (Stem Cell Technologies) supplemented with N2 (made in-house 2x stock in Advanced DMEM/F-12 (Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from blood using an EasySep Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, Canada), with 0.05% human serum albumin (HSA ...
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bioRxiv - Immunology 2021Quote: ... Human PBMC were purified from buffy coats from healthy donors by density separation using Lymphoprep (STEMCELL Technologies). PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM ...
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bioRxiv - Genomics 2020Quote: Human Embryonic stem cells (H1 – WiCell, WA01, lot # WB35186) were cultured in mTeSR1 media (StemCell Technologies, 85850) under feeder-free conditions on Matrigel H1-hESC-qualified matrix (Corning ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Genomics 2020Quote: ... T cells were purified using the EasySep™ Direct Human T Cell Isolation Kit (StemCell Technology #19661).
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bioRxiv - Microbiology 2020Quote: ... over irradiated murine stromal cells in human Long Term Culture Medium (hLTCM, MyeloCult H5100 [Stem Cell Technologies] supplemented with 1 μM hydrocortisone ...
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bioRxiv - Molecular Biology 2019Quote: ... CD4+ T-cells were isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... Negative selection of NK was performed on EasySep™ Human NK Cell Enrichment Kit system (StemCell Technologies). 2.106 sorted NK cells were cultured with 100 Gy-irradiated EBV-LCL cells in a 1:20 ratio ...
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bioRxiv - Immunology 2021Quote: ... CD34+ cells were enriched with the EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) without using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail ...
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bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... Purified pDCs were isolated via the EasySep™ Human Plasmacytoid DC Enrichment Kit (STEMCELL Technologies; Vancouver, Canada). Soluble or CQ-loaded nanocarriers at 3.91 μM total drug were cultured with 100,000 pDCs in RPMI 1640 with GlutaMAX plus 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were grown in suspension as spheres in flask in NeuroCult-XF Proliferation Medium Human (STEMCELL Technologies) with EGF and bFGF (10 ng/ml each ...
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bioRxiv - Cancer Biology 2022Quote: ... CD20+ target cells were isolated using the EasySep Human Biotin Positive Selection Kit II (StemCell Technologies, Canada) and the biotinylated anti-CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: Neutrophils from healthy donors were isolated with the EasySep™ Direct Human Neutrophil Isolation Kit (StemCell Technologies) as previously described (57,59) ...
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bioRxiv - Developmental Biology 2022Quote: Human induced pluripotent stem cells (iPSCs) from the EP1 line [43] were cultured in mTeSR1 (STEMCELL Technologies) on plates coated with Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning) ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils from HDs were purified by the EasySep Human Neutrophil Enrichment Kit (Stem Cell Technologies; > 99.7% purity) after centrifuging buffy coats onto Ficoll-Paque gradient density ...
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bioRxiv - Immunology 2020Quote: ... primary human NK cells were isolated by venipuncture followed by NK cell enrichment using RosetteSep (Stemcell Technologies) and separation by Ficoll-Paque density gradient ...
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bioRxiv - Microbiology 2021Quote: ... Human CD34+ HSC were isolated using a CD34+ HSC isolation kit (Stem Cell Technologies, Cambridge, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... H9 human ESCs were obtained from the WiCell Research Institute and cultured in mTeSR1 medium (STEMCELL Technologies). Embryoid bodies (EBs ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were isolated by negative selection (RosetteSep Human CD4+ T cell Enrichment Kit, Stemcell technologies) following the manufacturer’s procedure ...
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bioRxiv - Physiology 2019Quote: Monocytes were isolated from healthy apheresis donors by RosetteSep™ Human Monocyte Enrichment Cocktail (Stem Cell Technologies) by the University of Pennsylvania Human Immunology Core and provided as de-identified untraceable cells ...
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bioRxiv - Immunology 2020Quote: ... or αCD3/CD28 per manufacturer’s instructions (ImmuCult human CD3/CD28 T cell activator; StemCell Technologies, Cambridge, MA). After stimulation ...
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bioRxiv - Genomics 2020Quote: ... followed by immunomagnetic enrichment for the specific cell subset with EasySep Human cell enrichment kits (STEMCELL Technologies). B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: One mM of EDTA and 50 µL/mL RosetteSep human monocyte enrichment cocktail (STEMCELL Technologies, Vancouver, Canada) were added to blood and incubated for 20 minutes at room temperature before dilution with an equal volume of PBS enriched with 2% FCS ...
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bioRxiv - Genomics 2021Quote: ... Frozen vials of primary human CD4+CD45RO+ memory T cells were purchased from STEMCELL Technologies (Cat #70031).
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15) (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and CD4+ T cells were isolated using EasySep Human CD4+ T cell isolation kit (StemCell Technologies #17952). Cells were resuspended to 2.5 × 106 cells/mL in RPMI complete media supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from PBMCs using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies, BC). NK cells were co-cultured with K562-NL cells at different E:T ratios and treated with 10 μM of colistin for 5 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI-1640 (ATCC ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were purified from the PBMC population using the EasySep Human monocyte enrichment kit (STEMCELL Technologies; 19059), plated in 96-well plates and differentiated into microglia (iMDM ...
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bioRxiv - Immunology 2022Quote: ... coated with human type IV placental collagen in submerged PneumaCult-Ex Plus media (StemCell Technologies, Vancouver, Canada). When cells reached confluency ...
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies catalog # 100-0485) and were grown for 1 day with STEMdiff Neuronal induction medium (STEMCELL Technologies catalog # 05835) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... human iPSCs were routinely passaged as clumps onto Matrigel-coated plates with mTeSR+ media (StemCell Technologies #85850) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4 T cells were purified using the naïve human CD4+ T cell isolation kit (StemCell Technology) and stimulated with immobilized anti-human CD3 (5 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...