Labshake search
Citations for Stemcell Technologies :
351 - 400 of 2843 citations for ST2 Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... NK cells were purified by negative selection by using the EasySep™ Human NK cell Enrichment Kit (Stem Cell Technologies). 5×104 NK were co-cultured with NK sensitive cell line ...
-
bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
-
bioRxiv - Microbiology 2023Quote: ... Monocytes were isolated from PBMCs using EasySep Human monocyte enrichment kit without CD16 depletion (StemCell Technologies, Vancouver, British Columbia, Canada) according to the manufacturers’ instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... into meniscus-free SmartDish plates (6-well-plate, Stem Cell Technologies, 27370). To allow constant humidification ...
-
bioRxiv - Neuroscience 2024Quote: Commercially available microwell plates (AggreWellTM 400 24-well plate, 34415, StemCell Technologies) were used to generate retinal and thalamic spheroids (250 ...
-
bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
-
bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
-
bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
-
bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
-
bioRxiv - Genetics 2022Quote: ... AggreWellTM plates (Stemcell Technologies) were used ...
-
bioRxiv - Genetics 2019Quote: ... 10μl Human LDL (StemCell Technologies, 02698) for every 50ml media and the following cytokines (all from MiltenyiBiotec unless stated otherwise) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.02% Human LDL (Stem Cell Technologies) and the following cytokines (Miltenyi Biotec unless stated otherwise) ...
-
bioRxiv - Immunology 2020Quote: ... commercial cryo-human pDC (Stemcell Technology) from 5 healthy subjects ...
-
bioRxiv - Bioengineering 2023Quote: ... Human recombinant VEGF-165 (Stemcell technologies; Saint Egrève ...
-
bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... peripheral blood monocytes (PBMCs) were isolated from the whole blood of healthy donors using an EasySep Direct Human PBMC Isolation Kit (StemCell Technologies) through negative selection ...
-
bioRxiv - Microbiology 2019Quote: ... monocytes were isolated from thawed PBMCs using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (STEMCELL Technologies, Cat. No. 19058). The purified monocytes were seeded into a 24-well tissue culture treated plate at ~1.2 million cells/well in Advanced RPMI medium (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... Primary CD4+ or CD8+ T cells were isolated using EasySep Human CD4+ or CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
-
bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
-
bioRxiv - Bioengineering 2020Quote: HSPC (CD34+) were isolated from cord blood using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) according to the manufacture’s guidelines ...
-
bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
-
Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
-
bioRxiv - Immunology 2021Quote: Naïve IgM+IgD+B cells were purified by negative selection using the EasySepTM human naive B cell enrichment kit (19254; StemCell Technologies) from healthy subject PBMCs ...
-
bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
-
bioRxiv - Microbiology 2021Quote: ... total CD4+ T cells were isolated from PBMCs by negative magnetic separation using EasySep Human CD4+ T cell isolation kit (StemCell Technology). Following treatment for 5 days (Fig ...
-
bioRxiv - Immunology 2021Quote: Primary human CD4+ T cells were isolated from PBMCs using a Human resting CD4+ T Cells Isolation Kit I according to the manufacturer instructions (STEMCELL Technologies). Briefly ...
-
bioRxiv - Immunology 2019Quote: ... cryopreserved PBMCs were thawed (as above) and CD4 T cells were isolated by negative selection using the EasySep Human CD4 Enrichment Kit (Stemcell Technologies) before DNA extraction with the QiaAMP Blood Mini Kit (Qiagen) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
-
bioRxiv - Immunology 2019Quote: ... naïve CD44+ T cells were enriched by magnetic separation using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies). 55×104 T cells were cultured for 6 days in the presence of plate-bound αCD3ε (5 μg mL−1) ...
-
bioRxiv - Immunology 2021Quote: ... CD45+ cells were depleted from the same patient sample to obtain CD45-cells for co-culture using the EasySep Human CD45 Depletion Kit from StemCell Technologies Cat# 17898 ...
-
bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and CD4+ T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s instructions and purity of isolated CD4+ T-cells was controlled on a FACSverse™ flow cytometer (BD Biosciences ...
-
bioRxiv - Genomics 2021Quote: ... CD8+ T cells were purified from thawed PBMCs by negative selection using EasySep Human CD8+ T Cell Enrichment Kit (Cat#19053, STEMCELL Technologies) according to the manufacturer’s protocol.
-
bioRxiv - Genomics 2021Quote: ... B cells were purified from thawed PBMCs by negative selection using EasySep Human B Cell Enrichment Kit (Cat#19844, STEMCELL Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... Total CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag-2 magnet (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T-cells (CD3+CD4+CD45RA+CD45RO-) were purified from PBMC using the EasySep Human CD4+ T-cell Isolation Kit (Stemcell Technologies) to >95% purity according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... The negative fraction remaining after the positive selection of CD14+ cells was used for pan-DC enrichment employing the EasySep human pan-DC enrichment kit (StemCell Technologies). Total pan-DC were resuspended with 20,000 CD14+ cells and sent for sequencing ...
-
bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
-
bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Total CD3+ T cells were isolated from unfractionated PBMCs using the EasySep™ Human T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag™-2 magnet (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... Preliminary experiments were also performed after isolation of monocytes using the EasySep human monocyte enrichment kit with CD16 depletion (StemCell Technologies) and the EasySep human monocyte isolation kit (StemCell Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
-
bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
-
bioRxiv - Immunology 2020Quote: ... Total mDC and pDC were negatively enriched from PBMCs using the EasySep™ Human Pan-DC Pre-Enrichment Kit (StemCell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
-
bioRxiv - Genomics 2021Quote: Human ES cells were differentiated using the STEMdiff Definitive Endoderm Kit (TeSR-E8 Optimized) from STEMCELL Technologies (Ramme et al. 2019). Rat ES cells were differentiated following an optimized mouse protocols (Kim et al ...
-
bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
-
bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...