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Citations for Stemcell Technologies :
1 - 50 of 1401 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Neuroscience 2021Quote: ... supernatant was removed and re-suspended in complete BrainPhys™ (BrainPhys™ Neuronal Medium (Stemcell Technologies)+ 1% P/S + NeuroCult™ SM1 Neuronal Supplement (Stemcell Technologies) + N2 Supplement-A + 20 ng/ml recombinant human BDNF (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... + 1% P/S + NeuroCult™ SM1 Neuronal Supplement (Stemcell Technologies) + N2 Supplement-A + 20 ng/ml recombinant human BDNF (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... 1% P/S) supplemented with ROCK-inhibitor (5μM, StemCell Technologies). After 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were cleared for 1h in Optiprep (Stem Cell Technology). For dorsal imaging of the developing head ...
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bioRxiv - Neuroscience 2024Quote: ... ESCs were fed daily and were passaged every 3-4 days at a 1:6 ratio using ReLeSR™ (STEMCELL Technologies, #100-0483) following manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
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bioRxiv - Genetics 2022Quote: ... and 0.25µM LDN-193189 HCl (StemCell Technologies). On day 3 ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 3 × 10^6 cells were placed in each well of an AggreWell 800 plate (STEMCELL Technologies # 34811) containing Stemflex medium enriched with 1X Revita supplement ...
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bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged 1:6 using RELESR (StemCell Technologies 05872) for detachment of hESCs ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ng/mL IL-3 (STEMCELL Technologies, 78040) and 1% Penicillin-Streptomycin (Gibco 15140122) ...
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bioRxiv - Bioengineering 2021Quote: ... All human iPSCs were cultured on Matrigel (Corning)-coated 6-well plates with mTeSR™1 medium (StemCell Technologies). Full-volume medium replacement with mTESR1 was performed daily ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μM PD03259010 and 3 μM CHIR99021 (STEMCELL Technologies) and 100 U/ml penicillin ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
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bioRxiv - Cell Biology 2020Quote: ... coated culture dishes for 1h at 37°C with 5% CO2 using mTeSR1 medium (STEMCELL technologies). Cultures were routinely tested for mycoplasma contamination using MycoAlert Kit (Lonza) ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in mTeSR1 + 1% P/S and 10 µM Rock Inhibitor (Y-27632, StemCell Technologies, #72308). A full detailed protocol for cell line maintenance can be found at www.allencell.org/sops SOP ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... in conjunction with the RoboSep-S (STEMCELL Technologies) automated cell isolation machine and confirmed by flow analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Genomics 2021Quote: ... 25 000 cells were sorted into IntestiCult Organoid Growth Medium (0600-0, −2 and −3 combined, STEMCELL Technologies), centrifuged and directly processed for library generation with the 10x Genomics technology according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34815), using the recommended procedure by the supplier and at a density of 5000 cells per microwell ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were seeded at a 1:6 ratio and routinely passaged with Dispase (Stemcell Technologies).