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Citations for Stemcell Technologies :
301 - 350 of 478 citations for Recombinant Mouse TNFSF11 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Cell Biology 2023Quote: ... and lineage negative cell fraction were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies #19856). Cell sorting of LSK or LT-HSC ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
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bioRxiv - Genomics 2024Quote: ... mouse NIH-3T3 cells were washed once with PBS and dissociated with Accutase (Stem Cell Technologies, catalog no. 07920). Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck ...
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated by magnetic sorting (EasySep™ Mouse CD8+ T Cell Isolation Kit, StemCell Technologies), followed by tetramer E7 and TRM marker staining ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from total homogenate using the EasySep Mouse CD8+ T-cell (Stem Cell Technologies, 19853) isolation kit and EasyPlate EasySep magnets (Stem Cell Technologies ...
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bioRxiv - Immunology 2023Quote: ... dendritic cells (DCs) were enriched by magnetic isolation using the EasySepTM Mouse Pan-DC Enrichment Kit (Stem Cell Technologies). DCs were plated into 96-well U-bottom plates (100 × 106 cells/well) ...
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bioRxiv - Cancer Biology 2021Quote: ... naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) and stimulated with plate-bound α-CD3 (5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: Naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Bone marrow neutrophils were collected from the femur and tibia of naïve WT C57BL/6J and MPO-deficient mice using the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). 2×105 neutrophils were plated in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Molecular Biology 2021Quote: ... Vil-creER+/− and Ptbp1f/f mice as described [50] and cultured in IntestiCult Organoid Growth Medium (Mouse) (StemCell Technology, 06005). 4-hydroxytamoxifen was added into the culture medium at a final concentration of 200 nM to delete Ptbp1 ...
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bioRxiv - Genomics 2020Quote: ... CD4 and CD8 T cells were isolated from a mouse using CD4 and CD8 negative selection kits respectively (StemCell Technologies) and were labelled with the caged strand hybridized to the Ab-oligo conjugate ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Bioengineering 2020Quote: ... Initial enrichment for HSPC was performed with the EasySep Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (Stemcell Technologies, CA). Further isolation was performed to obtain a population of the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1mM EDTA-2Na).Lineage negative (Lin−) cells were enriched using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell technologies) and stained with fluorescent antibodies ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were isolated from whole splenocytes using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies, #19851), as per the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2021Quote: ... Isolation of hematopoietic lineage negative was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA). For cytotoxicity studies ...
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bioRxiv - Immunology 2020Quote: CD8 T cells were purified from spleens by negative selection using EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Bioengineering 2020Quote: ... Initial hematopoietic lineage negative enrichment was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA), followed by collection of the Lin- Sca-1+ c-kit+ (LSK ...
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bioRxiv - Immunology 2022Quote: ... Lm-OVA-specific CD8+ OT-I T cells were isolated from the spleen of infected mice by positive selection using the EasySep mouse CD90.1 positive selection kit (StemCell Technologies) as previously described (2 ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Neuroscience 2020Quote: ... carrying genes for tdTomato and the murine cell-surface protein CD90.2/Thy1.2 driven by the endogenous POU4F2 (BRN3B) promoter were clonally propagated in mTeSR-1 media (StemCell Technologies, Cambridge, MA) on growth factor-reduced Matrigel substrate (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated overnight in media supplemented with 5 µM of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (STEMCELL Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
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bioRxiv - Cancer Biology 2019Quote: Mouse CD3 positive T cells were isolated from spleens collected from CEA transgenic mice using negative selection kit (Stemcell Technologies, Canada) and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... Magnetic depletion of lineage-positive haematopoietic cells was performed using the EasySep mouse haematopoietic progenitor cell enrichment kit (Stem Cell Technologies), and lineage-depleted stem and progenitor cells were submitted to mass spectrometry analysis ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...