Labshake search
Citations for Stemcell Technologies :
501 - 550 of 1049 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
-
bioRxiv - Bioengineering 2019Quote: ... Initial HSPC isolation was performed using defined protocols of EasySep™ Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (#19756, #18000, Stemcell Technologies, CA). A secondary isolation further enriched the HSPC population by collecting the Lin− Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Cancer Biology 2020Quote: ... from wild-type or Jak1 floxed C57/Bl6 mice were isolated using EasySep mouse hematopoietic progenitor cell isolation kit (Stemcell technologies, Vancouver, Canada) which remove lineage marker-expressing cells with biotinylated antibodies directed against non-hematopoietic stem cells and non-progenitor cells (CD5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... the cells were needle triturated and resuspended in Mouse NSC Proliferation Media (1x Anti-Anti, 10ng/mL bFGF (#78003, Stem Cell Tech, Vancouver), 20ng/mL mEGF (#78016 ...
-
bioRxiv - Developmental Biology 2022Quote: ... and human naïve iPSCs (HPD06 and HPD01 were previously generated by direct reprogramming from somatic cells and described in13) were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs; DR4 ATCC) in PXGL medium36 or in RSeT medium (Stem Cell Technologies 05969). The PXGL medium was prepared as follows ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
-
bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Developmental Biology 2021Quote: NS cell cultures were established from E13.5 mouse ventral telencephalon as previously described (Conti et al. 2005) and maintained in mouse Neurocult basal media supplemented with proliferation supplement (Stem Cell Technologies, #05702,), 1% Penicillin-Streptomycin (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
-
bioRxiv - Immunology 2021Quote: Single cell suspensions were prepared from multiple OT-I and OT-I;IL-7Rα449F mice spleens and CD8 T cells were purified using the CD8 T cell negative selection kit (EasySep™ Mouse CD8+ T Cell Isolation Kit) from Stem Cell Technologies. 1×106 cells were transferred I.V ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
-
bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
-
bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2019) were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs; DR4 ATCC) in PXGL medium47 or in RSeT medium (Stem Cell Technologies 05969), at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... by following the Protocol described in the Technical Bulletin: Intestinal Epithelial Organoid Culture with IntestiCult Organoid Growth Medium (mouse) (StemCell Technologies, Document #28223). Organoids were maintained at 37°C and 5% CO2 as 3D spheroids in Matrigel (Corning ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
-
bioRxiv - Cell Biology 2024Quote: ... and iliac crests of each mouse. HSPCs were isolated using CD117 MicroBeads (Miltenyi cat. #130-091-224) and cultured in StemSpan SFEM II (StemCell Technologies cat. #09605) with 10 ng/mL recombinant murine SCF (STEMCELL Technologies ...
-
bioRxiv - Immunology 2024Quote: ... were cultured with lymphocytes (2.5 × 104) magnetically sorted frommousespleen singlecell preparations using MACs columns and the EasySep™ Mouse TCell Isolation Kit (StemCell Technologies Cat# 19851) for a total of 6d with 20 units/mL of IL-2 ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were passaged in small aggregates (1:7/1:10 depending on the line) using ReLeSR (StemCell Technologies). 10 μM Y-27632 (ROCK inhibitor ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tissues were minced into ∼1–2 mm pieces before digesting with 1 × Collagenase/Hyaluronidase (STEMCELL Technologies, Catalog #: 07912) and 100 μg/mL DNase I (Sigma Aldrich ...
-
bioRxiv - Genomics 2019Quote: ... and 1 mg/mL heparin (StemCell Technologies) and replaced every other day ...
-
bioRxiv - Microbiology 2021Quote: ... and mTeSRTM-1 medium (85850, StemCell Technologies). Cells were clump-passaged when colonies covered ∼75% of the wells by washing with PBS ...
-
bioRxiv - Bioengineering 2020Quote: ... in mTeSR™1 (Stem Cell Technologies). The established hiPSC colonies displayed a normal karyotype in culture ...
-
bioRxiv - Bioengineering 2020Quote: ... supplemented with 1 μM hydrocortisone (StemCell Technologies), 2 mM L-glutamine (ThermoFisher) ...
-
bioRxiv - Bioengineering 2020Quote: ... and 1 μM of StemRegenin (StemCell Technologies) before loading into the microfluidic device ...
-
bioRxiv - Cell Biology 2021Quote: ... serum-free medium mTESR-1 (StemCell technologies).
-
bioRxiv - Molecular Biology 2022Quote: ... + 1:1000 hydrocortisone stock solution (Stemcell Technologies) + 1uM A8301 (TGFβ inhibitor ...
-
bioRxiv - Immunology 2020Quote: ... CD38-FITC (clone AT-1, Stemcell Technologies), CD28-PE-Cy7 (clone CD28.2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... CHIR99021 (C, 1 mM; Stem Cell Technologies), (S)-(+)-Dimethindenemaleate (D ...
-
bioRxiv - Cell Biology 2021Quote: ... and 0.75μM StemRegenin 1 (StemCell Technologies 72342), at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... containing mTeSR™1 medium (STEMCELL Technologies) with blebbistatin (Calbiochem ...
-
bioRxiv - Cancer Biology 2021Quote: ... Rabbit anti-GFAP (STEMCELL Technologies; 1:500); Mouse anti-Beta Tubulin (STEMCELL Technologies ...
-
bioRxiv - Immunology 2020Quote: ... with 75 nM StemRegenin 1 (StemCell Technologies), 3.5 nM UM171 (StemCell Technologies) ...
-
bioRxiv - Cell Biology 2021Quote: ... Puromycin (2.5 μg mL-1; StemCell Technologies) was added for lines stably transduced with inducible mouse MLKL constructs ...
-
bioRxiv - Developmental Biology 2022Quote: ... using mTESR-1 medium (StemCell Technologies 85850) and grown for 5-8 days with daily medium replacement ...
-
bioRxiv - Systems Biology 2022Quote: ... and 1 μM Dabrafenib (Stem Cell Technologies) to induce differentiation ...
-
bioRxiv - Immunology 2023Quote: + 50 ng mL-1 mEGF (StemCell Technologies)
-
bioRxiv - Immunology 2023Quote: + 50 ng mL-1 mEGF (StemCell Technologies)
-
bioRxiv - Neuroscience 2023Quote: ... supplemented 1:10 with CloneR (Stemcell technologies) was added to the cuvette and the cells transferred to a 6-well plate ...
-
bioRxiv - Cell Biology 2023Quote: ... IFNy (25 ng ml-1, StemCell Technologies), TNFα (25 ng ml-1 ...
-
bioRxiv - Immunology 2023Quote: ... IGF-1 (40ng/mL; Stem Cell Technologies) and holo-transferrin (0.5 mg/mL ...
-
bioRxiv - Developmental Biology 2023Quote: ... in mTeSR-1 media (Stem Cell Technologies) in 5% CO2 at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... and TRA-1-60 (Stem Cell Technologies), which was measured at day 0 by flow cytometry (FCM ...
-
Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... 1 µM retinoic acid (72264, Stemcell Technologies) was added to the medium ...
-
bioRxiv - Neuroscience 2024Quote: ... 1 mL ReLeSR dissociation reagent (Stemcell Technologies) was added to the culture plate for 30 seconds at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1 µg/mL of doxycycline (StemCell Tech), and 10 mg/ml of human serum albumin (Sage) ...
-
bioRxiv - Cell Biology 2024Quote: ... + 1:1000 hydrocortisone stock solution (Stemcell Technologies) + 1μM A8301 (TGFb inhibitor ...