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Citations for Stemcell Technologies :
251 - 300 of 464 citations for Recombinant Mouse Enpp7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated from bone marrow (femurs and tibias) with the EasySepTM Mouse Monocyte Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... B cells were purified from a B6 mouse using an EasySep B cell negative selection kit (StemCell Technologies) and labelled with CFSE before being transferred at 4e6 per mouse ...
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bioRxiv - Immunology 2021Quote: ... β2-/- DCs were purified from potential other cells using the EasySepTM mouse Pan-DC enrichment kit (Stemcell Technologies) and allowed to rest for 1 h before the infection assay.
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bioRxiv - Neuroscience 2020Quote: Feeder-free E14Tg2a (Baygenomics) mouse embryonic stem cells (mES) were passaged on gelatin-coated (0.1% gelatin, StemCell Technologies) cell culture treated plastic dishes using established media and cell culture techniques (Sadegh and Macklis ...
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bioRxiv - Cancer Biology 2020Quote: ... Then DC were negatively selected using the EasySep Mouse Pan-DC Enrichment Kit (Stem Cell Technologies, Cat# 19763).
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched with the EasySep™ Mouse CD8 T Cell Isolation Kit (Stem Cell Technologies). Cells were stained ...
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Mouse Neutrophil Enrichment Kit was used as per the included protocol (StemCell Technologies, cat. 19762). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole murine blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Immunology 2021Quote: ... then neutrophils were negatively selected by using the EasySep™ mouse neutrophil enrichment kit (STEM cell technologies, #19762A), and 200,000 neutrophils/well were plated in 24-well plate on fibronectin coated glass coverslip ...
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bioRxiv - Immunology 2022Quote: Splenocyte-derived CD4+ T cells were isolated using Easysep Mouse CD4+ T cell isolation kit (19852, STEMCELL Technologies), and then 2e5 cells/well were plated in a 96 well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... and the hematopoietic progenitors were isolated by magnetic negative selection (Mouse Hematopoietic Progenitor Cell Isolation Kit, StemCell Technologies), and cultivated in SFEM II media supplemented with 50ng/ml of murine IL6 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cell isolation was performed using EasySep Mouse CD8+ T cell Isolation Kit (#19853, STEMCELL Technologies, USA) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured into 96-well low-attachment plates containing NeuroCult Basal Media (Mouse and Rat; STEMCELL Technologies) with Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Macrophages were isolated from the peritoneal lavage using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells were isolated with EasySep Mouse Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... then maintained in culture using reagents and protocol from the IntestiCult OGM Mouse Kit (Stemcell Technologies, Vancouver, Canada). Intestinal organoids were disassociated and resuspended into Corning® Matrigel® Growth Factor Reduced (GFR ...
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bioRxiv - Immunology 2023Quote: Splenic B cells or T cells were Isolated with EasySepTM Mouse B Cell Isolation Kit (#19854, StemCell Technologies) or EasySepTM Mouse T Cell Isolation Kit (#19851 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were purified from P14 spleens using EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies). Cells were then expanded using plate-bound CD3/CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... the resulting single cells were sorted on CD45 using EasySepTM Mouse CD45 Positive Selection Kit (StemCell Technologies, 18945) and used for downstream assays ...
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bioRxiv - Genetics 2024Quote: ... HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... mature microglial cells were isolated from astrocytes using EasySep Mouse CD11b Positive Selection Kit II (Stemcell Technologies #18970A,) and subsequently seeded into p60 plates ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were further lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... spleen-derived B-cells were selected using the EasySep™ Mouse Pan-B Cell Isolation Kit (STEMCELL Tech).
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bioRxiv - Cancer Biology 2021Quote: ... total CD8+ T cells were first enriched using the EasySep™ Mouse CD8+ T cell isolation kit (Stemcell Technologies) and then CD8+CD44hiCD62Llo central memory T cells purified by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were isolated for intravenous injection using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies, Canada). 4×106 isolated T cells were injected intravenously per mouse.
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bioRxiv - Immunology 2021Quote: ... Macrophages were separated from fibroblasts with the use of the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to phycoerythrin.
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bioRxiv - Immunology 2021Quote: ... Poly I:C-activated NK cells were isolated from the spleen using EasySep Mouse NK Cell Isolation Kit (STEMCELL Technologies). Purified NK cells were co-cultured with target cells at a ratio of 5:1 ...
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bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Immunology 2020Quote: ... splenic NK cells were enriched to >80% purity using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies), and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
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bioRxiv - Developmental Biology 2019Quote: ... Neurospheres were grown on non-tissue culture treated plates with mouse NeuroCult proliferation media (Stem Cell Technologies; Seattle, WA) containing 10 ng/ml bFGF ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched from the SCS using Mouse Pan-B Cell Isolation kits (Stemcell Technologies, Vancouver, BC, Canada). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Immunology 2019Quote: OT2 T cells were isolated by magnetic beads (EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit, StemCell Technologies) from a single cell suspension of splenocytes ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Cancer Biology 2021Quote: ... DF-AA27 GEMM GSCs were cultured in NeuroCult Basal Medium (Mouse and Rat) with Proliferation Supplement (StemCell Technologies 05702) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in wells of a 24-well plate containing 650 μl IntestiCult mouse organoid growth medium (StemCell Technologies cat# 06005) at 37°C/5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Cell Biology 2023Quote: ... and lineage negative cell fraction were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies #19856). Cell sorting of LSK or LT-HSC ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
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bioRxiv - Genomics 2024Quote: ... mouse NIH-3T3 cells were washed once with PBS and dissociated with Accutase (Stem Cell Technologies, catalog no. 07920). Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck ...