Labshake search
Citations for Stemcell Technologies :
551 - 600 of 695 citations for Recombinant Mouse Cd4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... T cells were isolated for intravenous injection using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies, Canada). 4×106 isolated T cells were injected intravenously per mouse.
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bioRxiv - Immunology 2021Quote: ... Macrophages were separated from fibroblasts with the use of the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to phycoerythrin.
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bioRxiv - Immunology 2021Quote: ... Poly I:C-activated NK cells were isolated from the spleen using EasySep Mouse NK Cell Isolation Kit (STEMCELL Technologies). Purified NK cells were co-cultured with target cells at a ratio of 5:1 ...
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bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Immunology 2020Quote: ... splenic NK cells were enriched to >80% purity using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies), and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
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bioRxiv - Developmental Biology 2019Quote: ... Neurospheres were grown on non-tissue culture treated plates with mouse NeuroCult proliferation media (Stem Cell Technologies; Seattle, WA) containing 10 ng/ml bFGF ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched from the SCS using Mouse Pan-B Cell Isolation kits (Stemcell Technologies, Vancouver, BC, Canada). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Cancer Biology 2021Quote: ... DF-AA27 GEMM GSCs were cultured in NeuroCult Basal Medium (Mouse and Rat) with Proliferation Supplement (StemCell Technologies 05702) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in wells of a 24-well plate containing 650 μl IntestiCult mouse organoid growth medium (StemCell Technologies cat# 06005) at 37°C/5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Cell Biology 2023Quote: ... and lineage negative cell fraction were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies #19856). Cell sorting of LSK or LT-HSC ...
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bioRxiv - Genomics 2024Quote: ... mouse NIH-3T3 cells were washed once with PBS and dissociated with Accutase (Stem Cell Technologies, catalog no. 07920). Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck ...
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated by magnetic sorting (EasySep™ Mouse CD8+ T Cell Isolation Kit, StemCell Technologies), followed by tetramer E7 and TRM marker staining ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from total homogenate using the EasySep Mouse CD8+ T-cell (Stem Cell Technologies, 19853) isolation kit and EasyPlate EasySep magnets (Stem Cell Technologies ...
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bioRxiv - Immunology 2023Quote: ... dendritic cells (DCs) were enriched by magnetic isolation using the EasySepTM Mouse Pan-DC Enrichment Kit (Stem Cell Technologies). DCs were plated into 96-well U-bottom plates (100 × 106 cells/well) ...
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bioRxiv - Cancer Biology 2021Quote: ... naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) and stimulated with plate-bound α-CD3 (5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: Naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Bone marrow neutrophils were collected from the femur and tibia of naïve WT C57BL/6J and MPO-deficient mice using the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). 2×105 neutrophils were plated in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Molecular Biology 2021Quote: ... Vil-creER+/− and Ptbp1f/f mice as described [50] and cultured in IntestiCult Organoid Growth Medium (Mouse) (StemCell Technology, 06005). 4-hydroxytamoxifen was added into the culture medium at a final concentration of 200 nM to delete Ptbp1 ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Bioengineering 2020Quote: ... Initial enrichment for HSPC was performed with the EasySep Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (Stemcell Technologies, CA). Further isolation was performed to obtain a population of the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1mM EDTA-2Na).Lineage negative (Lin−) cells were enriched using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell technologies) and stained with fluorescent antibodies ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were isolated from whole splenocytes using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies, #19851), as per the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2021Quote: ... Isolation of hematopoietic lineage negative was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA). For cytotoxicity studies ...
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bioRxiv - Immunology 2020Quote: CD8 T cells were purified from spleens by negative selection using EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Bioengineering 2020Quote: ... Initial hematopoietic lineage negative enrichment was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA), followed by collection of the Lin- Sca-1+ c-kit+ (LSK ...
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bioRxiv - Immunology 2022Quote: ... Lm-OVA-specific CD8+ OT-I T cells were isolated from the spleen of infected mice by positive selection using the EasySep mouse CD90.1 positive selection kit (StemCell Technologies) as previously described (2 ...
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
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bioRxiv - Cancer Biology 2019Quote: Mouse CD3 positive T cells were isolated from spleens collected from CEA transgenic mice using negative selection kit (Stemcell Technologies, Canada) and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... Magnetic depletion of lineage-positive haematopoietic cells was performed using the EasySep mouse haematopoietic progenitor cell enrichment kit (Stem Cell Technologies), and lineage-depleted stem and progenitor cells were submitted to mass spectrometry analysis ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Microbiology 2021Quote: ... 5% CO2 for 15-20 minutes to allow the Matrigel to polymerize and then 550 μl of pre-warmed complete IntestiCult Growth Medium (mouse) (6005; STEMCELL Technologies) containing 500 nM Y-27632 (10005583 ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Isolated cells were grown in a semi-solid methyl cellulose matrix for the Mouse Hematopoietic CFU Assay in Methocult Media 3630 (STEMCELL Technologies), following the manufacturer’s instructions ...