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Citations for Stemcell Technologies :
501 - 550 of 994 citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... feeder-free maintenance medium (mTeSR for human ES and iPS cells, Stem Cell Technologies, cat#85850) as previously described (Low et al. ...
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bioRxiv - Genomics 2019Quote: ... H1 human ESCs (WA01, WiCell Research Institute) were maintained in feeder-free mTesR1 medium (Stemcell Technologies). hESCs (passage 26 ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Genomics 2019Quote: ... neutrophils were further purified to homogeneity using an EasySep Human Neutrophil Enrichment kit (Stemcell technologies, 19257) as per the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and T cells isolated using the EasySep Human T Cell Isolation kit (Stemcell Technologies, #17951). Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
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bioRxiv - Neuroscience 2022Quote: ... human neuron-astrocyte co-cultures were maintained in neuron maintenance medium (1X BrainPhys Basal (StemCell Technology), 1X B27 with Vitamin A (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSCs (IMR90-4; WiCell Research Institute) were maintained with mTeSR Plus (STEMCELL Technologies, 100-0276) on dishes coated with 9-10 μg/cm2 Growth Factor Reduced Matrigel (Corning ...
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bioRxiv - Immunology 2022Quote: ... NK cells isolated from buffy coats using an EasySep human NK cell enrichment kit (StemCell Technologies) were added to the mixed target population at a 1:1 effector:target ratio ...
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bioRxiv - Immunology 2022Quote: ... NGFR+ cells were bead-purified by an EasySep Human CD271 Positive Selection Kit II (STEMCELL Technologies). See Fig ...
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bioRxiv - Immunology 2022Quote: Frozen human bone marrow mononuclear cells (BMMC) from three healthy donors were purchased from STEMCELL Technologies and stored in liquid nitrogen until use ...
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bioRxiv - Immunology 2022Quote: ... NK cells were enriched from PBMCs by EasySep™ Human NK Cell Isolation Kit (StemCell Technologies) and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... All human CD34+ HSPCs were cultured in StemSpan SFEM II medium (STEMCELL Technologies, Cambridge, MA, USA) supplemented with stem cell factor (SCF ...
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bioRxiv - Bioengineering 2023Quote: Magnetic sorting for CD19 using the EasySep human CD19 positive selection kit II (StemCell Technologies Inc.) involved the same dissociation procedure as above ...
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bioRxiv - Cell Biology 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained on vitronectin XFTM (10 μg/mL, StemCell Technologies)-coated plates and in Essential 8 (E8 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were purified by negative selection using RosetteSepTM Human T cell enrichment cocktail (STEMCELL Technologies) following manufacturer’s instructions ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... CD34+ HSPC were positively selected using the EasySepTM Human CD34 Positive Selection Kit II (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... to collect mononuclear cells then purified using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). HUCB CD34+ cells were then seeded in Matrigel Matrix (Cat# 356231 ...
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bioRxiv - Microbiology 2023Quote: ... primary human monocyte derived macrophages were detached from 12-well plate by using accutase (Stemcell Technologies). Digested macrophages were washed with PBS for 2 times in 96-well plates and fixed in fixation buffer (1% paraformaldehyde (PFA) ...
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bioRxiv - Immunology 2023Quote: ... human CD4+ T cells were purified from frozen peripheral blood mono-nuclear cells (PBMCs) (STEMCELL Technologies) with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... CD34+ ECs were isolated using a Human cord blood CD34 Positive selection kit II (StemCell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: B cell isolation was performed using the the EasySep Human B Cell Isolation Kit (StemCell Technologies). PBMC were diluted in PBS supplemented with 2 % FBS and 1 mM EDTA to a final concentration of 107 cells/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... 5g were isolated from human peripheral blood mononuclear cells (PBMCs) (StemCell Technologies, 70025, individual donors selected) using EasySepTM Human CD11b Positive Selection and Depletion Kit (StemCell Technologies ...
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cells were then isolated using a human B cells enrichment kit (Stemcell Technologies, Cat#19054). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: Human mesoderm cells were derived from hESCs using the STEMdiff mesoderm induction medium (Stem Cell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Primary T cells were isolated from PBMCs using EasySep Human T Cells Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic stem cells (hESCs; H1) were maintained in mTeSR1 plus media (StemCell Technologies, 100-0276) on Matrigel (Corning ...
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bioRxiv - Immunology 2024Quote: Human peripheral blood leukopaks from de-identified healthy donors were purchased from STEMCELL Technologies (Vancouver, Canada). CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Cancer Biology 2021Quote: ... Human fetal tissue derived U5 NCSs were cultured in NeuroCult NS-A basal medium (Stem Cell Technologies) supplemented with N2 (made in-house 2x stock in Advanced DMEM/F-12 (Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from blood using an EasySep Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, Canada), with 0.05% human serum albumin (HSA ...
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bioRxiv - Immunology 2021Quote: ... Human PBMC were purified from buffy coats from healthy donors by density separation using Lymphoprep (STEMCELL Technologies). PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM ...
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bioRxiv - Genomics 2020Quote: Human Embryonic stem cells (H1 – WiCell, WA01, lot # WB35186) were cultured in mTeSR1 media (StemCell Technologies, 85850) under feeder-free conditions on Matrigel H1-hESC-qualified matrix (Corning ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Genomics 2020Quote: ... T cells were purified using the EasySep™ Direct Human T Cell Isolation Kit (StemCell Technology #19661).
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bioRxiv - Microbiology 2020Quote: ... over irradiated murine stromal cells in human Long Term Culture Medium (hLTCM, MyeloCult H5100 [Stem Cell Technologies] supplemented with 1 μM hydrocortisone ...
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bioRxiv - Molecular Biology 2019Quote: ... CD4+ T-cells were isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... Negative selection of NK was performed on EasySep™ Human NK Cell Enrichment Kit system (StemCell Technologies). 2.106 sorted NK cells were cultured with 100 Gy-irradiated EBV-LCL cells in a 1:20 ratio ...
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bioRxiv - Immunology 2021Quote: ... CD34+ cells were enriched with the EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) without using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail ...
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bioRxiv - Bioengineering 2021Quote: ... Purified pDCs were isolated via the EasySep™ Human Plasmacytoid DC Enrichment Kit (STEMCELL Technologies; Vancouver, Canada). Soluble or CQ-loaded nanocarriers at 3.91 μM total drug were cultured with 100,000 pDCs in RPMI 1640 with GlutaMAX plus 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were grown in suspension as spheres in flask in NeuroCult-XF Proliferation Medium Human (STEMCELL Technologies) with EGF and bFGF (10 ng/ml each ...
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bioRxiv - Cancer Biology 2022Quote: ... CD20+ target cells were isolated using the EasySep Human Biotin Positive Selection Kit II (StemCell Technologies, Canada) and the biotinylated anti-CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: Neutrophils from healthy donors were isolated with the EasySep™ Direct Human Neutrophil Isolation Kit (StemCell Technologies) as previously described (57,59) ...