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Citations for Stemcell Technologies :
551 - 600 of 1068 citations for Recombinant Human LAG3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... to collect mononuclear cells then purified using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). HUCB CD34+ cells were then seeded in Matrigel Matrix (Cat# 356231 ...
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bioRxiv - Microbiology 2023Quote: ... primary human monocyte derived macrophages were detached from 12-well plate by using accutase (Stemcell Technologies). Digested macrophages were washed with PBS for 2 times in 96-well plates and fixed in fixation buffer (1% paraformaldehyde (PFA) ...
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bioRxiv - Bioengineering 2023Quote: Magnetic sorting for CD19 using the EasySep human CD19 positive selection kit II (StemCell Technologies Inc.) involved the same dissociation procedure as above ...
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bioRxiv - Cell Biology 2023Quote: ... All human CD34+ HSPCs were cultured in StemSpan SFEM II medium (STEMCELL Technologies, Cambridge, MA, USA) supplemented with stem cell factor (SCF ...
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bioRxiv - Cell Biology 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained on vitronectin XFTM (10 μg/mL, StemCell Technologies)-coated plates and in Essential 8 (E8 ...
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bioRxiv - Immunology 2023Quote: ... human CD4+ T cells were purified from frozen peripheral blood mono-nuclear cells (PBMCs) (STEMCELL Technologies) with EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... CD34+ ECs were isolated using a Human cord blood CD34 Positive selection kit II (StemCell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: B cell isolation was performed using the the EasySep Human B Cell Isolation Kit (StemCell Technologies). PBMC were diluted in PBS supplemented with 2 % FBS and 1 mM EDTA to a final concentration of 107 cells/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... 5g were isolated from human peripheral blood mononuclear cells (PBMCs) (StemCell Technologies, 70025, individual donors selected) using EasySepTM Human CD11b Positive Selection and Depletion Kit (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: ... B cells were then isolated using a human B cells enrichment kit (Stemcell Technologies, Cat#19054). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Primary T cells were isolated from PBMCs using EasySep Human T Cells Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Human primary CD34+ HSPCs from G-CSF-mobilized healthy adult donors were obtained from StemCell Technologies. CD34+ HSPCs were expanded ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (STEMCELL Technologies), and activated with anti-CD3/anti-CD28 Dynabeads (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and 1.78x PBS equilibration buffer and the EasySep Direct Human Neutrophil Isolation kit (StemCell Tech #19666). The kits were used as suggested by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: Human iPSC cells (WTSli002-A, Wellcome trust) were maintained on Vitronectin XF™ (07180, Stemcell Technologies) coated 6-well plates (ref ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in mTeSR plus (Cat# 100-0276, StemCell Technologies) on Matrigel-coated plates (Cat# 354230 ...
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bioRxiv - Immunology 2024Quote: ... cells were purified from blood using an EasySep Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, Canada) according to manufacturer’s directions ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Cancer Biology 2021Quote: ... Human fetal tissue derived U5 NCSs were cultured in NeuroCult NS-A basal medium (Stem Cell Technologies) supplemented with N2 (made in-house 2x stock in Advanced DMEM/F-12 (Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from blood using an EasySep Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, Canada), with 0.05% human serum albumin (HSA ...
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bioRxiv - Immunology 2021Quote: ... Human PBMC were purified from buffy coats from healthy donors by density separation using Lymphoprep (STEMCELL Technologies). PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM ...
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bioRxiv - Genomics 2020Quote: Human Embryonic stem cells (H1 – WiCell, WA01, lot # WB35186) were cultured in mTeSR1 media (StemCell Technologies, 85850) under feeder-free conditions on Matrigel H1-hESC-qualified matrix (Corning ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Genomics 2020Quote: ... T cells were purified using the EasySep™ Direct Human T Cell Isolation Kit (StemCell Technology #19661).
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bioRxiv - Microbiology 2020Quote: ... over irradiated murine stromal cells in human Long Term Culture Medium (hLTCM, MyeloCult H5100 [Stem Cell Technologies] supplemented with 1 μM hydrocortisone ...
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bioRxiv - Molecular Biology 2019Quote: ... CD4+ T-cells were isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... Negative selection of NK was performed on EasySep™ Human NK Cell Enrichment Kit system (StemCell Technologies). 2.106 sorted NK cells were cultured with 100 Gy-irradiated EBV-LCL cells in a 1:20 ratio ...
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bioRxiv - Immunology 2021Quote: ... CD34+ cells were enriched with the EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) without using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail ...
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bioRxiv - Bioengineering 2021Quote: ... Purified pDCs were isolated via the EasySep™ Human Plasmacytoid DC Enrichment Kit (STEMCELL Technologies; Vancouver, Canada). Soluble or CQ-loaded nanocarriers at 3.91 μM total drug were cultured with 100,000 pDCs in RPMI 1640 with GlutaMAX plus 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were grown in suspension as spheres in flask in NeuroCult-XF Proliferation Medium Human (STEMCELL Technologies) with EGF and bFGF (10 ng/ml each ...
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bioRxiv - Cancer Biology 2022Quote: ... CD20+ target cells were isolated using the EasySep Human Biotin Positive Selection Kit II (StemCell Technologies, Canada) and the biotinylated anti-CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: Neutrophils from healthy donors were isolated with the EasySep™ Direct Human Neutrophil Isolation Kit (StemCell Technologies) as previously described (57,59) ...
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bioRxiv - Developmental Biology 2022Quote: Human induced pluripotent stem cells (iPSCs) from the EP1 line [43] were cultured in mTeSR1 (STEMCELL Technologies) on plates coated with Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning) ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils from HDs were purified by the EasySep Human Neutrophil Enrichment Kit (Stem Cell Technologies; > 99.7% purity) after centrifuging buffy coats onto Ficoll-Paque gradient density ...
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bioRxiv - Immunology 2020Quote: ... primary human NK cells were isolated by venipuncture followed by NK cell enrichment using RosetteSep (Stemcell Technologies) and separation by Ficoll-Paque density gradient ...
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bioRxiv - Microbiology 2021Quote: ... Human CD34+ HSC were isolated using a CD34+ HSC isolation kit (Stem Cell Technologies, Cambridge, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... H9 human ESCs were obtained from the WiCell Research Institute and cultured in mTeSR1 medium (STEMCELL Technologies). Embryoid bodies (EBs ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were isolated by negative selection (RosetteSep Human CD4+ T cell Enrichment Kit, Stemcell technologies) following the manufacturer’s procedure ...
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bioRxiv - Physiology 2019Quote: Monocytes were isolated from healthy apheresis donors by RosetteSep™ Human Monocyte Enrichment Cocktail (Stem Cell Technologies) by the University of Pennsylvania Human Immunology Core and provided as de-identified untraceable cells ...
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bioRxiv - Immunology 2020Quote: ... or αCD3/CD28 per manufacturer’s instructions (ImmuCult human CD3/CD28 T cell activator; StemCell Technologies, Cambridge, MA). After stimulation ...
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bioRxiv - Genomics 2020Quote: ... followed by immunomagnetic enrichment for the specific cell subset with EasySep Human cell enrichment kits (STEMCELL Technologies). B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: One mM of EDTA and 50 µL/mL RosetteSep human monocyte enrichment cocktail (STEMCELL Technologies, Vancouver, Canada) were added to blood and incubated for 20 minutes at room temperature before dilution with an equal volume of PBS enriched with 2% FCS ...
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bioRxiv - Genomics 2021Quote: ... Frozen vials of primary human CD4+CD45RO+ memory T cells were purchased from STEMCELL Technologies (Cat #70031).
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... The NSC media was composed of Neurocult NS-A basal medium human (Stem Cell Technologies, Cat #05750) along with L-glutamine (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and CD4+ T cells were isolated using EasySep Human CD4+ T cell isolation kit (StemCell Technologies #17952). Cells were resuspended to 2.5 × 106 cells/mL in RPMI complete media supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from PBMCs using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies, BC). NK cells were co-cultured with K562-NL cells at different E:T ratios and treated with 10 μM of colistin for 5 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI-1640 (ATCC ...