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Citations for Stemcell Technologies :
151 - 200 of 1037 citations for Recombinant Human CD3e Molecule Epsilon CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells were isolated by RosetteSep (STEMCELL Technologies).
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bioRxiv - Immunology 2019Quote: ... and Immunocult anti-CD3/28 activator (Stemcell Technologies; 10971) for 4 days ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies), or a combination of plate-bound anti-CD3 (OKT3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Neuroscience 2023Quote: ... the natural developmental signaling factors were recapitulated with small molecules including Ascorbic acid (72132, StemCell technologies), CHIR99021 (72054 ...
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bioRxiv - Immunology 2022Quote: ... and 30 μl of additional biotinylated anti-CD3 (STEMCELL Technologies, 18051) was added to the sample to improve purity ...
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bioRxiv - Genomics 2024Quote: CD3+ T cells were isolated from healthy donor leukopaks (Stemcell Technologies) using bead separation (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... CD3+ T cells were isolated by magnetic bead separation (StemCell Technology) from PBMCs of three leukopaks (Cambridge Biosciences ...
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bioRxiv - Immunology 2019Quote: OT1 and OTII T cells were isolated from LN of TCR transgenic mice using either a CD8 or CD4 StemSep enrichment kit (STEMCELL Technologies), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... Leukemic blasts were isolated from whole blood using CD3 depletion (StemCell Technologies) and ficoll density gradient separation (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... activated with CD3/CD28 T cell Activator reagent (STEMCELL Technologies, Vancouver, Canada) and transduced on day 1 with the CAR lentiviral particles in the presence of 1X Transdux (Systems Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: PDECs were treated with 25ul/mL anti-CD3/CD28/CD2 (Stemcell Technologies), 100ug/mL Atezolizumab (Selleck Chemicals) ...
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bioRxiv - Neuroscience 2021Quote: ... the media with small molecules was transitioned to mTeSR1 full stem cell maintenance media (Stemcell Technologies Cat#85850) with the media being changed daily ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... CD3-CD19- mononuclear cells were enriched by Lymphoprep (Stem Cell Technologies, Grenoble, France) density gradient centrifugation followed by Magnetic-activated cell sorting using biotin MicroBead kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... and the following mouse monoclonal antibodies: anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) and anti-Ku80 (BD Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... magnetically isolated rCD4 (CD3+/CD4+/CD69-/ CD25-/ HLA-DR-) T cells (Stemcell Technologies) were rested in the presence of antiretroviral drugs (10nM Raltegravir and 20nM Efavirenz) ...
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bioRxiv - Microbiology 2020Quote: ... Anti-CD3/CD28 antibody-coated beads (ImmunoCult) were purchased from STEMCELL technologies (Vancouver, CA).
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bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Immunology 2022Quote: ... CD3+ cells were depleted using the negative selection cocktail RosetteSep™ (STEMCELL Technologies Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequent isolation of CD3+ T cells using negative selection isolation kits (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The iPSCs were subjected to monolayer Dual SMAD inhibition by changing the media to Neural Induction Media (NIM) which is composed of NBM supplemented with small molecules SB431542 (10μM, an inhibitor of TGFβ pathway) (72232, Stem Cell Technologies) and LDN193189 (0.1μM ...
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bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng/mL recombinant murine SCF (StemCell Technologies cat. # 78064), and 1 mg/mL polyvinyl alcohol (Sigma cat ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Genomics 2021Quote: ... The T cell expansion medium was composed of 12.5ul/ml ImmunoCult CD3/CD28 (STEMCELL Technologies) and 100ng/ml IL-2 and 5ng/ml IL-15 (PeproTech EC Ltd) ...
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bioRxiv - Immunology 2020Quote: ... T cells were activated with anti-CD3/anti-CD28 tetrameric antibody complexes (Stemcell Technologies, #10971) on days 1 and 13 of culture and expanded every 3-4 days ...
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bioRxiv - Bioengineering 2019Quote: ... 5.0 × 106 cryopreserved T cells were expanded using CD3/CD28 T cell activator (StemCell Technologies) and standard conditions for 16 days to achieve sufficient cell numbers for the experimental workflow ...
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bioRxiv - Immunology 2021Quote: ... or a custom stem cell kit that removes CD66b+/GPA+/CD3+/CD14+ cells (StemCell Technologies). Enriched fractions were then stained using the fluorescently conjugated antibodies IgD-FITC (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... CD4+ or CD3+ cells were enriched from leukopheresis products through negative selection (RosetteSep, STEMCELL Technologies) before density gradient centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... Human T cells from anonymous donors sourced by STEMCELL Technologies were isolated by negative selection for CD3+ populations as per manufacturer’s instruction (EasySep, STEMCELL Technologies), and cultured and prepared identically to murine T cells using corresponding reagents for human cells.
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were activated for 72 hours with ImmunoCult T cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... primary CD4+ T cells were activated with anti-CD3/CD28 antibodies (cat#10971, Stemcell Technologies). 1e6 activated primary CD4+ T cells were electroporated with 1μg PEmax variant mRNA ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... T-cells were isolated with an EasySep CD3+ enrichment kit from StemCell Technologies (Vancouver, BC Canada), per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Genetics 2023Quote: ... was purchased as a single guide molecule from IDT (Integrated DNA Technologies) to be complexed with ArchiTect™ Cas9 nuclease (Stem Cell Technologies) for editing experiments in primary human CD34+ HSPCs.
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bioRxiv - Neuroscience 2022Quote: iPSC derived astrocytic cells (hiAC) were differentiated from NPCs by small molecule differentiation in STEMdiff™ Astrocyte Differentiation Media (Stem Cell Tech #cat100-0013) on Corning® Matrigel® coated tissue culture treated plates ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Microbiology 2019Quote: Harvested human blood was incubated with RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies) or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... human PBMC (ST70025, STEMCELL Technologies) was mixed and incubated with MACS CD4 MicroBeads (130-045-101 ...
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bioRxiv - Cell Biology 2022Quote: ... IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™ Organoid Differentiation Medium (Human) were used for culture or differentiation of human gut organoids (STEMCELL Technologies, Vancouver, Canada). Organoids were used prior to passage 3 for optimal efficiency of EEC and β-like cell induction.
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bioRxiv - Immunology 2020Quote: Primary human NK cells were enriched from the peripheral blood of human donors using RosetteSep Human NK cell Enrichment Cocktail (Stem Cell Technologies, Cat #15065) and cultured overnight in RPMI-1640 (Corning ...