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Citations for Stemcell Technologies :
101 - 150 of 1011 citations for Recombinant Human CD28 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 30 ng/mL human recombinant epidermal growth factor (rhEGF) (STEMCELL Technologies 78006) and filtered through a 40 μm filter (Fisherbrand™ 22-363-547 ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Immunology 2023Quote: ... Anti-CD3/CD28/CD2 Immunocult (Stemcell Technologies) was used for T-cell activation ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Neuroscience 2023Quote: ... plated cells were immediately supplemented with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Immunology 2020Quote: ... cells in full media were incubated with Human Recombinant IFNα-2A (10ng/mL, StemCell Technologies 78076.1) or IFNγ (10ng/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 μg human recombinant bFGF and 10 μg 0.2% heparin solution (all from STEMCELL Technologies, Canada).
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15) (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...
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bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies), or a combination of plate-bound anti-CD3 (OKT3 ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Biophysics 2021Quote: ... 05702) and media supplemented with 20ng/ml of human recombinant epithelial growth factor (EGF - STEMCELL Technologies, Cat. No. 78006). All cells were grown in a humidified incubator atmosphere at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured on recombinant human VTN (rhVTN) coated 60mm plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Bioengineering 2021Quote: ... and 25 ng/mL BMP4 with 50 ng/mL human recombinant Vascular Endothelial Growth Factor 165 (VEGF-165, STEMCELL Technologies, 78073) for 2 days ...
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bioRxiv - Physiology 2021Quote: ... mice fed a normal chow diet were injected with vehicle (saline or PBS), 300U/kg or 3000U/kg of recombinant human erythropoietin (EPREX, Janssen-Cilag) or 50ng/g IL-1β (STEMCELL Technologies). All animal use complied with the guidelines of the Canadian Committee for Animal Protection and was approved by IRCM Animal Care Committee.
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Cancer Biology 2021Quote: ... activated with CD3/CD28 T cell Activator reagent (STEMCELL Technologies, Vancouver, Canada) and transduced on day 1 with the CAR lentiviral particles in the presence of 1X Transdux (Systems Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: PDECs were treated with 25ul/mL anti-CD3/CD28/CD2 (Stemcell Technologies), 100ug/mL Atezolizumab (Selleck Chemicals) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Microbiology 2020Quote: ... Anti-CD3/CD28 antibody-coated beads (ImmunoCult) were purchased from STEMCELL technologies (Vancouver, CA).
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bioRxiv - Genomics 2021Quote: ... The T cell expansion medium was composed of 12.5ul/ml ImmunoCult CD3/CD28 (STEMCELL Technologies) and 100ng/ml IL-2 and 5ng/ml IL-15 (PeproTech EC Ltd) ...
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bioRxiv - Immunology 2020Quote: ... T cells were activated with anti-CD3/anti-CD28 tetrameric antibody complexes (Stemcell Technologies, #10971) on days 1 and 13 of culture and expanded every 3-4 days ...
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bioRxiv - Bioengineering 2019Quote: ... 5.0 × 106 cryopreserved T cells were expanded using CD3/CD28 T cell activator (StemCell Technologies) and standard conditions for 16 days to achieve sufficient cell numbers for the experimental workflow ...
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bioRxiv - Biochemistry 2023Quote: ... primary CD4+ T cells were activated with anti-CD3/CD28 antibodies (cat#10971, Stemcell Technologies). 1e6 activated primary CD4+ T cells were electroporated with 1μg PEmax variant mRNA ...
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bioRxiv - Immunology 2022Quote: ... Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies) and incubated 3 days at 37°C in humidified 5% CO2.
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng/mL recombinant murine SCF (StemCell Technologies cat. # 78064), and 1 mg/mL polyvinyl alcohol (Sigma cat ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Bioengineering 2019Quote: ... and an “activated” population that was cultured in medium supplemented with 25 μl/ml tetrameric antibody against CD2/CD3/CD28 (StemCell Technologies). Quiescent and activated T cell populations were cultured separately for 48 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...