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Citations for Stemcell Technologies :
401 - 450 of 982 citations for Recombinant Human CD274 His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 20ng/mL human plasma-derived low-density lipoprotein (hLDL) (Stemcell Technologies, Cat. No. 02698), 1uM StemReginin1 (SR1 ...
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bioRxiv - Cell Biology 2023Quote: ... with EasySep Human CD4+ or CD8+ T Cell Isolation Kits (STEMCELL Technologies Inc., USA). Unless otherwise indicated ...
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bioRxiv - Immunology 2023Quote: ... HIV-negative donors using the RosetteSep Human CD4 T cell enrichment kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Human iPSCs were grown in mTeSR Plus medium (100-0276, StemCell Technologies, Vancouver, Canada) on tissue culture treated plates coated with Vitronectin (A31804 ...
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bioRxiv - Bioengineering 2024Quote: ... human T cells were obtained from fresh Leukopaks purchased from STEMCELL Technologies (Seattle, WA). T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: Human iNKT cells were enriched by magnetic bead-based positive selection (Stem Cell Technologies EasySep™ Release Human PE Positive Selection Kit ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were subsequently isolated using the EasySep™ Human Monocyte Isolation Kit (StemCell Technologies) in accordance with the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated using the EasySep Human CD4 Positive Selection Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 12.5 uL/mL of Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) and 10 ng/mL of CTS IL-2 (Thermofisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... The media was then replaced with hTSC media containing 200 ng/ml recombinant B18R to prevent gamma-interferon response (Stem Cell Technologies; Cat. No. 78075). For reprogramming experiments lipofection was performed at the same time every day for 20 days ...
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bioRxiv - Molecular Biology 2021Quote: ... and activated with Immunocult™ human CD3/CD28 T cell activator (Stemcell Technologies, Vancouver, Canada), in the RPMI-1640 media supplemented with 2mM L-Glutamine ...
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bioRxiv - Cancer Biology 2020Quote: BT088 and BT054 cells9 were grown in Human Neurocult proliferation media (Stem Cell Tech; # 05751) containing human epidermal growth factor (hEGF ...
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bioRxiv - Bioengineering 2020Quote: ... CD8+ cells were isolated using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) following manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... we respectively used mouse- and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Bioengineering 2020Quote: Human T cells were obtained by purchasing whole blood units from STEMCELL technologies (Vancouver, Canada). STEMCELL reported that these products are distributed using Institutional Review Board (IRB)-approved consent forms and protocols ...
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bioRxiv - Bioengineering 2020Quote: ... CD8+ T cells were isolated using RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve primary human T cells (CD3+/CD4+/CD45RA+) were also sourced from STEMCELL Technologies (#70029) and cultured in RPMI with 10% FBS and IL-2 as described above ...
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bioRxiv - Bioengineering 2022Quote: ... T cells were collected using negative selection (Stemcell Technologies EasySep Human T Cell Isolation Kit) from autologous healthy donor PBMCs ...
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... gradient centrifugation using the RosetteSep Human NK Cell Enrichment Cocktail (STEMCELL Technologies, Vancouver, BC, Canada).
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bioRxiv - Cell Biology 2021Quote: Human cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) were purchased (StemCell Technologies #78003) or acquired through the Binns Program for Cord Blood Research at Stanford University under informed consent ...
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bioRxiv - Cancer Biology 2021Quote: Human Peripheral Blood Pan-T Cells was purchased from Stem Cell Technologies (Vancouver, BC, Canada) and Panc-1 cells were obtained from the Kansas University Medical Center ...
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bioRxiv - Genetics 2022Quote: ... CD34+ HSPCs were enriched by EasySep Human Cord Blood CD34+ positive selection kit (StemCell Technologies). Enriched cells were nucleofected by AAVS1 control and MYSM1 sgRNAs ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated using the EasySep Human NK Cell Isolation Kit (Stem Cell Technologies) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated from PBMCs using an EasySep Human Monocyte Isolation Kit (all STEMCELL Technologies).
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bioRxiv - Immunology 2019Quote: ... Total blood DCs were enriched with EasySep human pan-DC pre-enrichment kit (Stemcell Technologies). DC enriched fraction were stained with antibodies specific for HLA-DR APCeFluor780 ...
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bioRxiv - Bioengineering 2019Quote: ... before adding 250μL/well of either human IntestiCult™ Organoid Growth Medium (STEMCELL Technology, #06010) or human organoid basal culture media consisting of conditioned media produced using stably transfected L cells (Wnt 50% ...
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bioRxiv - Immunology 2020Quote: ... or after NK cell enrichment using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies). To measure antigen-specific NKCL responses ...
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bioRxiv - Cancer Biology 2019Quote: ... according to the manual for Human Colony-forming Unit (CFU) Assays Using MethoCult (StemCell Technologies).
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bioRxiv - Genomics 2021Quote: ... hematopoietic colony-forming unit were automated counted and analyzed by STEMvision™ Human (STEMCELL Technologies). Differentiation frequency was calculated for each sorted population by number of resulting colonies/numbers of starting cells seeded.
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bioRxiv - Cancer Biology 2021Quote: ... CD3+ T cells were isolated using EasySep Human T Cell Isolation Kit (STEM CELL Technologies) and then cultured in CTS T Cell Expansion medium (Thermo ...
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bioRxiv - Molecular Biology 2019Quote: ... Monocytes were isolated from PBMCs using the EasySep Human Monocyte Enrichment Kit (STEMCELL Technologies, 19058) and were differentiated in RPMI medium (Gibco ...
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bioRxiv - Physiology 2021Quote: ... The colonoids were maintained in Human IntestiCult Organoid Growth Medium (STEMCELL Technologies, Inc, Vancouver, BC). The human colonoids were transfected with the plasmids for fluorescent protein tagged human ALS-causing mutation SOD1G93A-GFP ...
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bioRxiv - Genomics 2021Quote: ... T cells were purified using the RosetteSep Human T Cell Enrichment Kit (Stem Cell Technologies) with the Lymphoprep density gradient medium and SepMate-50 tubes ...
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bioRxiv - Immunology 2022Quote: ... Total blood DCs were enriched with EasySep human pan-DC preenrichment kit (Stemcell Technologies 19251). DC enriched fraction were stained with antibodies specific for HLA-DR APCeFluor780 ...
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bioRxiv - Immunology 2020Quote: Cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, STEMCELL Technologies). Purified NK cells were then cultured in stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...
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bioRxiv - Immunology 2021Quote: Human peripheral blood from de-identified healthy donors was purchased from STEMCELL Technologies (Vancouver, Canada), which collects and distributes de-identified human blood products with consent forms ...
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bioRxiv - Immunology 2021Quote: ... T cells were further enriched using the EasySep Human T Cell Isolation Kit (STEMCELL Technologies), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Enriched HSPC-pDCs were then primed for 1-3 days in RF10 (RPMI-1640 medium (Merck ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Obtained cells were phenotypically validated by flow cytometry.
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bioRxiv - Cancer Biology 2021Quote: ... human CD41+ cells were isolated using the CD41+ selection TAC kit (Cat# 10450, StemCell Technologies).
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bioRxiv - Immunology 2022Quote: ... and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology, #10970) with or without 2µM of NU74411 for 48 hours.
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were magnetically isolated (#100-0694 EasySep human positive selection kit II, STEMCELL Technologies) and the resulting cell suspension was passed through a 70 μm cell strainer.
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bioRxiv - Immunology 2022Quote: ... NK cells were removed from cultures (EasySep Human CD56 Positive Selection Kit II, StemCell Technologies). Remaining CD4 T cells were analyzed by flow cytometry to confirm removal of NK cells by staining for CD56 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) by immunomagnetic negative selection according to manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were isolated using EasySepTM Direct Human T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 5iLA naive human PSCs were dissociated into single cells by incubation with Accumax (Stemcell technologies) for 3 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... plated in matrigel and cultured with 500 μl/well human IntestiCult™ OGM (Stemcell Technologies). Medium was changed every 2-3 days and organoids were passaged once a week ...