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Citations for Stemcell Technologies :
101 - 138 of 138 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were removed and cells were resuspended in Noci-2 medium + CEPT and plated at 5.5 x 106 cells/well into 6-well AggreWell 800 plates according to manufacturer’s instruction (STEMCELL Technologies) for free-floating suspension culture and nocisphere formation ...
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bioRxiv - Neuroscience 2023Quote: ... at a density of 30 x 104 cells/well and cultured in NeuroCult neuronal plating medium (Stem Cell Technologies) supplemented with B-27 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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bioRxiv - Genomics 2024Quote: ... 24 x 106 SNRPN-2A-GFP VP64dCas9VP64 (maternal) or dCas9KRAB (paternal) iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850 ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Genomics 2021Quote: ... cells were centrifuged (350 x g for 5 minutes) and resuspended in 5 mL of prewarmed 0.25% trypsin/EDTA (#07901, Stemcell Technologies) at 37°C for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 5% CO2 for 48 hours prior to electroporation at a seeding concentration of 4.0 x 105 cells/mL in HSPC culture media (StemSpan SFEM II (StemCell Technologies) supplemented with penicillin/streptomycin (100 U/mL ...
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bioRxiv - Immunology 2021Quote: ... and plated on Matrigel-coated plates at a density of 5 x 105 cells per cm2 in mTESR1 medium (StemCell Technologies) supplemented with 10 ng/mL of the RHO/ROCK pathway inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 200 x g for 5 min prior to be resuspended in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and seeded onto growth-factor-reduced Matrigel™-coated plates (8.6µg/cm2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML samples were grown in AML growth media consisting of X-VIVO with 20% BIT Serum Substitute (Stem Cell Technologies) or StemSpan Serum Free Expansion Media II (SFEM II ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Cell Biology 2023Quote: ... and sub-plated at 25 x 104 cells/ml on dishes coated with 50 μg/ml poly-L-ornithine in BrainPhys neuronal medium (STEMCELL Technologies) containing N2 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Cells were counted and adjusted to a concentration of 5 x 105 cells/mL with ALDEFLUOR assay (01700; Stem Cell Technologies) buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 106 lineage negative cells/ml were transduced with 1 x 108 TU/ml of empty vector or Vegfc lentivirus in serum-free Stem-Span medium (StemCell Technologies) supplemented with 10 ng/ml IL-3 ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 0.5-1 x 107 cells/ml in “phase 1 expansion medium” consisting of Stemspan SFEM (Stem Cell Technologies) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Neuroscience 2023Quote: ... matrix protein coated plates and cultured in mTeSR™ medium (STEMCELL tech), passaged using Versene (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... 8 x 105 cells were resuspended in 100 μl Ingenio-solution (Mirrus) supplemented with Rock inhibitor (10 μM, Y-27632, Stem Cell Technologies) and 2.5 μg of CRISPR:hSpCas9 plasmid and 2.5 μg linearized DNA targeting plasmid and immediately pulse with program DC100 (using a 4D-Nucleofector ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
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bioRxiv - Bioengineering 2021Quote: ... coiled-coil containing protein kinase (ROCK) inhibitor (5 μM, StemCell Technologies, Vancouver, BC, Canada). Until 95% confluency was reached ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 50 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Stem Cell Technologies). The cell suspension was distributed into ultra-low attachment 96-well plates (Corning) ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
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bioRxiv - Neuroscience 2020Quote: ... carrying genes for tdTomato and the murine cell-surface protein CD90.2/Thy1.2 driven by the endogenous POU4F2 (BRN3B) promoter were clonally propagated in mTeSR-1 media (StemCell Technologies, Cambridge, MA) on growth factor-reduced Matrigel substrate (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated overnight in media supplemented with 5 µM of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (STEMCELL Technologies).
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bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...