Labshake search
Citations for Stemcell Technologies :
1 - 50 of 1399 citations for Rat Dual Specificity Mitogen Activated Protein Kinase Kinase 7 MAP2K7 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... coiled-coil containing protein kinase (ROCK) inhibitor (5 μM, StemCell Technologies, Vancouver, BC, Canada). Until 95% confluency was reached ...
-
bioRxiv - Molecular Biology 2024Quote: ... a Rho-associated kinase (ROCK) inhibitor (StemCell Technologies), to promote cell survival ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 50 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Stem Cell Technologies). The cell suspension was distributed into ultra-low attachment 96-well plates (Corning) ...
-
bioRxiv - Cell Biology 2020Quote: ... Rho-kinase inhibitor Y-27632 (STEMCELL Technologies, 20 µM), MLCK inhibitor ML-7 (Enzo ...
-
bioRxiv - Molecular Biology 2023Quote: ... Rho-associated kinase (ROCK)-Inhibitor (StemCell Technologies, Y-27632) was added to mTeSR at 10 µM ...
-
bioRxiv - Synthetic Biology 2021Quote: ... neural maintenance media with Rho Kinase Inhibitor IV (Stemcell Technologies Australia ...
-
bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
-
bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
-
bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were collected and seeded at 1.5 × 105 cells/cm2 cell density on glass coverslips with Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor supplemented mTeSR media (5 μM, StemCell Technologies). The culture was maintained with daily media changes until 90% confluency was reached ...
-
bioRxiv - Bioengineering 2023Quote: ... and then incubated overnight in media supplemented with 5 µM of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (STEMCELL Technologies).
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
-
bioRxiv - Cell Biology 2022Quote: ... and plated in media containing 10 μM Rho-kinase inhibitor Y-27632 (StemCell Technologies). Embryonic body (EB ...
-
bioRxiv - Neuroscience 2021Quote: ... and media with 10 μM Rho-associated kinase (ROCK) inhibitor (Y-27632, STEMCELL Technologies) was used for any single-cell dissociation ...
-
bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10 μM Rho-kinase inhibitor (RockI; Y-27632 STEMCELL Technologies, cat. 72304). iPSCs were seeded at a density of 6×104 cells/cm2 and expanded for 3 days to achieve 80% confluency prior to differentiation ...
-
bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL Rho-kinase inhibitor (RockI; Y-27632 STEMCELL Technologies, cat. 72304). To prepare the differentiation experiments ...
-
bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
-
Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
-
bioRxiv - Genomics 2021Quote: ... after which cells were replated in E8 containing p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (2 μM; Stemcell Tech, 72307).
-
bioRxiv - Cell Biology 2019Quote: ... cells were harvested with GCDR and plated in Essential-E8 supplemented with 10 uM Rho-kinase inhibitor Y27632 (Stem Cell Technologies, 72304) on VTN coated 6-well culture dish to yield approximately 80% confluence next day ...
-
bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
-
bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
-
bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
-
bioRxiv - Microbiology 2021Quote: ... IFNα was quantified using the pan-IFNα ELISA kit (Stem Cell Technologies). IFNβ was quantified using human IFN-beta Quantikine ELISA kit (R&D Systems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with NeuroCult proliferation kit (mouse & rat, STEMCELL Technologies, #05702), human recombinant EGF (20 ng/mL ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dual SMAD inhibitors (2μM Dorsomorphin; StemCell Tech; #72102 ...
-
bioRxiv - Immunology 2021Quote: ... Poly I:C-activated NK cells were isolated from the spleen using EasySep Mouse NK Cell Isolation Kit (STEMCELL Technologies). Purified NK cells were co-cultured with target cells at a ratio of 5:1 ...
-
bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
-
bioRxiv - Genetics 2021Quote: Add normal rat serum from the EasySep Mouse T-cell Isolation Kit (StemCell Technologies) and Isolation Cocktail ...
-
bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
-
bioRxiv - Neuroscience 2023Quote: Patterned differentiation of iPSCs toward neural progenitor cells (NPCs) was conducted using dual-SMAD inhibition (STEMdiff SMADi Neural Induction Kit, STEMCELL Technologies). To promote complete and uniform generation of NPCs ...
-
bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
-
bioRxiv - Physiology 2021Quote: The EPO concentrations from 34 serum samples were determined using the Human Erythropoietin ELISA Kit from Stemcell Technologies. Briefly ...
-
bioRxiv - Cancer Biology 2021Quote: ... activated with CD3/CD28 T cell Activator reagent (STEMCELL Technologies, Vancouver, Canada) and transduced on day 1 with the CAR lentiviral particles in the presence of 1X Transdux (Systems Biosciences) ...
-
bioRxiv - Immunology 2021Quote: ... and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705 ...
-
bioRxiv - Bioengineering 2021Quote: ... and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies). T cells were cultured at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... following a monolayer culture method with a commercial dual SMAD inhibition-mediated neural induction medium (STEMdiff™ SMADi Neural Induction Kit, Stem Cell Technologies) based on a published protocol59 ...
-
bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
-
bioRxiv - Cancer Biology 2021Quote: ... and activated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Stem Cell Technologies) following the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... activated and expanded in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies Inc., USA) with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2 ...
-
bioRxiv - Immunology 2023Quote: ... Human PBMCs were activated with ImmunocultTM CD3/CD28 Human T cell Activators (STEMCELL Technologies, anti-human CD3 and anti-human CD28 monospecific antibody complexes ...
-
bioRxiv - Cell Biology 2023Quote: ... activated and expanded in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies Inc., USA) with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and activated with Immunocult™ human CD3/CD28 T cell activator (Stemcell Technologies, Vancouver, Canada), in the RPMI-1640 media supplemented with 2mM L-Glutamine ...
-
bioRxiv - Immunology 2020Quote: ... T cells were activated with anti-CD3/anti-CD28 tetrameric antibody complexes (Stemcell Technologies, #10971) on days 1 and 13 of culture and expanded every 3-4 days ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...