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Citations for Stemcell Technologies :
401 - 450 of 4518 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Microbiology 2019Quote: ... Isolated PBMCs were used to isolate CD4+ T-cells by negative selection using the EasySep ™ Human CD4+ T-cells isolation kit (Stem Cell Technologies, Canada). Briefly ...
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bioRxiv - Immunology 2021Quote: ... CD34+ peripheral stem cells were isolated from peripheral blood and expanded using the Complete Kit for Human Whole Blood CD34+ Cells (Stem Cell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and activated with Immunocult™ human CD3/CD28 T cell activator (Stemcell Technologies, Vancouver, Canada), in the RPMI-1640 media supplemented with 2mM L-Glutamine ...
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bioRxiv - Cancer Biology 2020Quote: BT088 and BT054 cells9 were grown in Human Neurocult proliferation media (Stem Cell Tech; # 05751) containing human epidermal growth factor (hEGF ...
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bioRxiv - Immunology 2021Quote: ... we respectively used mouse- and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Bioengineering 2020Quote: Human T cells were obtained by purchasing whole blood units from STEMCELL technologies (Vancouver, Canada). STEMCELL reported that these products are distributed using Institutional Review Board (IRB)-approved consent forms and protocols ...
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... gradient centrifugation using the RosetteSep Human NK Cell Enrichment Cocktail (STEMCELL Technologies, Vancouver, BC, Canada).
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bioRxiv - Cell Biology 2021Quote: Human cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) were purchased (StemCell Technologies #78003) or acquired through the Binns Program for Cord Blood Research at Stanford University under informed consent ...
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bioRxiv - Immunology 2020Quote: Cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, STEMCELL Technologies). Purified NK cells were then cultured in stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Cancer Biology 2021Quote: ... human CD41+ cells were isolated using the CD41+ selection TAC kit (Cat# 10450, StemCell Technologies).
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bioRxiv - Immunology 2022Quote: ... and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology, #10970) with or without 2µM of NU74411 for 48 hours.
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bioRxiv - Immunology 2022Quote: ... NK cells were removed from cultures (EasySep Human CD56 Positive Selection Kit II, StemCell Technologies). Remaining CD4 T cells were analyzed by flow cytometry to confirm removal of NK cells by staining for CD56 ...
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bioRxiv - Developmental Biology 2022Quote: ... 5iLA naive human PSCs were dissociated into single cells by incubation with Accumax (Stemcell technologies) for 3 min at 37 °C ...
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Autologous humanized PDX modeling for immuno-oncology recapitulates the human tumor microenvironmentbioRxiv - Cancer Biology 2022Quote: ... CD34+ cells were positively selected using the EasySep Human CD34 Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Human blood-derived mast cells were plated between 40,000–50,000/well in StemSpan (StemCell Technologies) serum-free medium supplemented with Stem Cell Factor (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... EasySep™ human CD34 positive selection kit II was procured from Stem cell technologies (17856). Ficoll® Paque PLUS density gradient media was purchased from Sigma Aldrich (GE17-1440-03).
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bioRxiv - Neuroscience 2023Quote: ... Human DS and control iPSCs were cultured and maintained in mTeSR1 medium (Stem Cell Technologies) in tissue culture dishes coated with hESC-qualified Matrigel (Corning) ...
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bioRxiv - Immunology 2023Quote: ... we used mouse-specific and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PBMCs were activated with ImmunoCult human CD3/CD28 T cell activator (STEMCELL Technologies; Cat. # 10971) and 50 U/mL IL-2 (BD-Pharmingen ...
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bioRxiv - Bioengineering 2023Quote: ... Human induced pluripotent stem cell (hPSC) line PGP1 was cultured with mTeSR1 (STEMCELL Technologies, #85850) in tissue culture 6-well plates coated with reduced growth factor Geltrex (Gibco ...
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bioRxiv - Microbiology 2023Quote: A three-stage protocol was used to differentiate human bone marrow CD34+ cells (STEMCELL Technologies) down the erythroid lineage as previously described.37 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... H1 human embryonic stem-cell (H1-hESC) line was cultured in mTeSR1 medium (STEMCELL Technologies) under 5% CO2 at 37℃ and was sub-cultured when reaching 80% confluence ...
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bioRxiv - Molecular Biology 2024Quote: G-CSF Mobilized Human Peripheral Blood CD34+ Cells were purchased from StemCell Technologies (Catalog: 70060.1). Cells were thawed by resuspending in 10 mL of RPMI without FBS ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with 25μL/mL ImmunoCult Human αCD2/αCD3/αCD28 T cell Activator (STEMCELL Technologies) or 10μL/mL T-cell TransAct αCD3/αCD28 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... primary patient samples and healthy controls were plated in methylcellulose (Stem Cell Technologies H4435, Vancouver, CA) with the CA-4948 and control ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The purity of cultured CTC subpopulation was validated by gating the sorted cells with conjugated primary antibodies of CD44-FITC (Stem Cell Technologies), CD24-PE ...
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bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Immunology 2021Quote: Naïve IgM+IgD+B cells were purified by negative selection using the EasySepTM human naive B cell enrichment kit (19254; StemCell Technologies) from healthy subject PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Microbiology 2021Quote: ... total CD4+ T cells were isolated from PBMCs by negative magnetic separation using EasySep Human CD4+ T cell isolation kit (StemCell Technology). Following treatment for 5 days (Fig ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Immunology 2019Quote: ... naïve CD44+ T cells were enriched by magnetic separation using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies). 55×104 T cells were cultured for 6 days in the presence of plate-bound αCD3ε (5 μg mL−1) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and CD4+ T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s instructions and purity of isolated CD4+ T-cells was controlled on a FACSverse™ flow cytometer (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... CD8+ T cells were purified from thawed PBMCs by negative selection using EasySep Human CD8+ T Cell Enrichment Kit (Cat#19053, STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: ... B cells were purified from thawed PBMCs by negative selection using EasySep Human B Cell Enrichment Kit (Cat#19844, STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T-cells (CD3+CD4+CD45RA+CD45RO-) were purified from PBMC using the EasySep Human CD4+ T-cell Isolation Kit (Stemcell Technologies) to >95% purity according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...