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Citations for Stemcell Technologies :
351 - 400 of 4519 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... human T cells were obtained from fresh Leukopaks purchased from STEMCELL Technologies (Seattle, WA). T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... 12.5 uL/mL of Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) and 10 ng/mL of CTS IL-2 (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were isolated from PBMCs via the Easysep Human CD4+ T-cell negative enrichment kit (STEMCELL Technologies). The isolated human CD4+ T cells were cultured at 2.5 × 106 cells/ml in complete RPMI medium (cRPMI) ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from PBMCs with EasySep™ Human T cell Isolation Kit per manufacturer’s instructions (StemCell Technologies). Isolated T cells (0.5-1 × 105 per well ...
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coats using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... ICM T cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activators (25 μl/ml, STEMCELL Technologies). TAC T cells were stimulated with T Cell CD3/CD28 TransAct™ ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using EasySep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5x10 6 cells/mL left overnight for recovery ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified from thawed PBMCs using the EasySep Human T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cell line HS181 (hPSCreg Kle001-A) was maintained on vitronectin (VTN-XL; Stem Cell Technologies, 07180) in Essential-E8 Medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: CD4+T-cells were isolated from PBMCs with the EasySep™ human CD4+T-cell enrichment kit (StemCell Technologies). T-cells/PBMCs were added to cultured hepatic epithelial cell lines (Huh-7 ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T cells were enriched by negative selection using the EasySep Human CD4+ T Cell Enrichment Kit (StemCell Technologies). The purity of the CD4+ T cells was evaluated by flow cytometry and was higher than 95% ...
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bioRxiv - Cell Biology 2020Quote: ... centrifugation were enriched for CD34+ cells using the RosetteSep™ Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... we treated CD4+ T cells with either αCD3αCD28 (ImmunoCult™ Human CD3/CD28 T Cell Activator; STEMCELL Technologies, 10971) or IFN I (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and resting CD4+ T cells (rCD4s) (EasySep™ Human Resting CD4+ T Cell Isolation Kit, Cat # 17962, StemCell Technologies) were isolated from HIV-1 aviremic donors living with HIV-1 ...
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bioRxiv - Immunology 2022Quote: ... Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies). Prior to infection ...
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bioRxiv - Molecular Biology 2022Quote: Myeloid cells were purified from PBMCs using Easy Sep Human Myeloid positive selection kit (Stem cell technology; Cat# 18653). The kit positively selects myeloid cells positive for CD33 and CD66b ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were enriched using the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies, Cat. No. 19052) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... were seeded (70,000 cells each) in medium for long-term culture of human cells (catalogue no. 05150; STEMCELL Technologies) supplemented with hydrocortisone in collagen-coated plates ...
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bioRxiv - Immunology 2023Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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bioRxiv - Immunology 2023Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
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bioRxiv - Immunology 2023Quote: ... T cells were obtained from whole blood using the RosetteSep™ Human T Cell Enrichment Cocktail (StemCell Technologies, 15061), followed by density gradient separation using Ficoll® Paque Plus (Cytiva Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated from the samples using RosetteSep Human B cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies, Cat# 17951, lOT #1000153615). CD3+ T-cells were thawed ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... Primary CD4+ and CD8+ T cells were isolated by negative selection (STEMCELL Technologies #15062 & 15063). CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130 ...
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bioRxiv - Bioengineering 2023Quote: ... primary rat hepatocytes were plated in AggreWell Micromolds (400μm square AggreWell micromolds, Stem Cell Technologies) along with NHDFs at a 1:1.6 ratio and incubated in a hepatocyte medium containing DMEM with L ...
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bioRxiv - Biochemistry 2023Quote: ... primary CD4+ T cells were activated with anti-CD3/CD28 antibodies (cat#10971, Stemcell Technologies). 1e6 activated primary CD4+ T cells were electroporated with 1μg PEmax variant mRNA ...
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bioRxiv - Physiology 2019Quote: Monocytes were isolated by the University of Pennsylvania Human Immunology Core from healthy apheresis donors with institutional review board approval by RosetteSep™ human monocyte enrichment cocktail (Stem Cell Technologies, Vancouver, Canada), and MΦs were differentiated by 12-days adherence culture in RPMI 1640 media containing 10% human serum and 1x cell culture pen/strep antibiotic mix (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2021Quote: ... NK cell isolation was done by using the negative selection kit “RosetteSep™ Human NK Cell Enrichment Cocktail” (STEMCELL Technologies) according to the company’s protocol ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... and T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were further isolated through beads-mediated negative selection (EasySep Human CD4+ T cell Isolation Kit, Stemcell technology) and resuspended in Trizol (Ambion ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cell isolation was performed through beads-mediated negative selection (EasySep Human CD4+ T Cell Isolation Kit, Stemcell Technologies) and CD4+ cell purity was assessed by flow cytometry.
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bioRxiv - Genetics 2019Quote: ... lineage positive cells were depleted by negative selection with the StemSep Human Hematopoietic Progenitor Cell Enrichment Kit (StemCell Technologies, 14056) and Anti-Human CD41 TAC (Stem Cell ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cell Biology 2021Quote: ... co-D12 or –D14 CD34+ cells were isolated using a human CD34 positive selection kit (Stem Cell Technologies, Vancouver, Canada). CD34+ cells (1 × 105/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were obtained from PBMCs using the EasySep human CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France). Next ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... total CD4+ T cells were isolated using a combination of the human CD4+ T cell RosetteSep™ kit (STEMCELL Technologies) and Ficoll-Paque (Cytiva Inc. ...