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Citations for Stemcell Technologies :
601 - 650 of 1051 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mg/mL DNase 1 (Stem Cell Technologies). The samples were digested for 1 hour at 37°C with constant low agitation using a thermomixer (Eppendorf) ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll Paque Plus solution and density gradient centrifugation using SepMate tubes (StemCell Technologies). All samples were stored in vapor-phase liquid nitrogen after cryopreservation with 10% dimethyl sulfoxide until analysis ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... Briefly, PB was collected in FACS buffer (PBS, 2 % FBS, 2 mM EDTA) supplemented with 0.0004 % heparin solution (STEMCELL Technologies Inc.). RBCs was removed by incubation at 37 °C for 25 minutes with 1 % Dextran solution in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... triturated with a 1000 µl pipette and dissociated in a collagenase solution with 1 U of Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... The epidermis was separated from the dermis by placing the strips in tubes containing 10 ml of 5U/ml Dispase II (Stem Cell Technologies, USA) and incubated at 4°C overnight and at 37°C for 2-3 hours ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Bioengineering 2022Quote: ... after which the peripheral mononuclear cell fraction was isolated by carefully layering 25 mL diluted buffy coat onto 13 mL Lymphoprep (07851, StemCell technologies, Cologne, Germany) in separate 50 mL centrifugal tubes ...
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bioRxiv - Neuroscience 2023Quote: ... and subplated at 25x104 cells/ml on dishes coated with 50 ug/ml Poly-L-ornithine in BrainPhys neuronal medium (Stem cell technologies, USA) containing N2 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... after which the peripheral mononuclear cell fraction was isolated by carefully layering 25 mL diluted buffy coat onto 13 mL Lymphoprep (07851, StemCell technologies, Cologne, Germany) in separate 50 mL centrifugal tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Bioengineering 2024Quote: ... The solution was diluted 1:1 with dilution medium and 30 mL was carefully layered on top of 15 mL of Lymphoprep (Cat # 07811, STEMCELL Technologies, Vancouver, Canada) solution ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with phosphate-buffered saline (PBS) (5 ml) and detached by addition of ‘Gentle Dissociation Reagent’ (3 ml) (STEMCELL Technologies; Cat. No. 07174) stored at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Developmental Biology 2022Quote: ... Roughly separated tubules were incubated in collagenase type IV in DMEM (5 mL for 1 testis pair, 1 mg/mL, STEMCELL Technologies Inc., Cat# 07909) at 37 °C while rotating ...
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bioRxiv - Immunology 2022Quote: ... A single-step density gradient centrifugation was performed to isolate peripheral blood mononuclear cells (PBMCs) by slowly layering diluted blood (30 mL) over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) contained within either a 50 mL SepMate™ (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... 8 x 105 cells were resuspended in 100 μl Ingenio-solution (Mirrus) supplemented with Rock inhibitor (10 μM, Y-27632, Stem Cell Technologies) and 2.5 μg of CRISPR:hSpCas9 plasmid and 2.5 μg linearized DNA targeting plasmid and immediately pulse with program DC100 (using a 4D-Nucleofector ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: Tumours and matched lung metastases were dissociated into single cells using mechanical disruption followed by enzymatic digestion by a collagenase-hyaluronidase solution (StemCell Technologies; 07912) for 1 h at 37°C without mechanical agitation ...
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bioRxiv - Immunology 2022Quote: ... blood samples were diluted with an equal amount of phosphate buffer saline solution (PBS) and slowly layered on top of the same volume of Lymphoprep density gradient medium (Stemcell Technologies Inc.). After that ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were mechanically detached in VP spheroid medium using a serological pipette before transfer to a 6WP pre-coated with anti-adherence solution (STEMCELL Technologies, 07010). Detached cells from five wells of a 6WP were added divided over six wells of a 6WP ...
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bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Immunology 2021Quote: ... Lymphoprep (d = 1.077 g mL−1, StemCell Technologies ™, Canada) was underlaid and PBMC were isolated by density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... in RPMI) and 50ng/mL CSF-1 (STEMCELL Technologies, Canada) in petri dishes (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... containing 1μg/ml ROCK inhibitor Y-27632 (Stem Cell Technologies). Cells were incubated at 37°C for 24 h prior to incubation with DMEM/F12 medium (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... at a concentration of 106 cells/ml (STEMCELL Technologies, #5854) using a CoolCell LX freezing block (Biocision ...
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bioRxiv - Systems Biology 2020Quote: ... plus 1 mg/mL DNase I (Stem Cell Technologies 07900) for 2 min ...
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bioRxiv - Bioengineering 2021Quote: ... and 4µg/mL low density lipoproteins (Stem Cell Technologies #02698), 55 µM 2-mercaptoethanol (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and recombinant human EGF (10 ug/mL; Stem Cell Technologies) in 10% DMSO before long-term cryopreservation (1X107 cells/mL ...
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bioRxiv - Genetics 2022Quote: ... iPSCs were treated with 1 U/ml dispase (StemCell Technologies) and transferred to uncoated T-25 flasks in mTESR media supplemented with 10 μM ROCK inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... Each well had 1 mL StemSpan SFEM (StemCell Technologies, Inc.) with 20 ng/mL recombinant murine SCF (PeproTech ...
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bioRxiv - Cancer Biology 2021Quote: ... basic fibroblast growth factor (20ng/mL; STEMCELL Technologies Cat#78006), heparin (2 mg/mL 0.2% Heparin Sodium Salt in PBS ...
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bioRxiv - Genomics 2020Quote: ... and 10-20 μg/mL DNAse I (StemCell Technologies, #7900) for 5-10 min in a 37°C water bath with intermittent mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μg/ml collagen type I (Stemcell Technologies, Catalog # 07001), 50 μg/ml penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... contained within either a 50 mL SepMate™ (STEMCELL Technologies) or a 50 mL conical tube ...
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bioRxiv - Immunology 2022Quote: ... and 1 mg/mL collagenase IV (StemCell Technologies, BC, Canada) for 30 min at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were dissociated with 1 mL Accutase (STEMCELL Technologies, 07920) for 5 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... containing 0.5 ml of ReproTeSR™ medium (#05921, STEMCELL Technologies). After two days ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg/mL of DNase 1 (STEMCELL Technologies, Cat. # 07900) was added to the cell suspension for 15 minutes at room temperature to prevent cell clumping ...
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bioRxiv - Immunology 2023Quote: ... Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies, 07923), 0.1 mg/mL DNase I (Sigma-Aldrich ...