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Citations for Stemcell Technologies :
1 - 50 of 806 citations for Polyhomeotic Like Protein 1 PHC1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CD38-FITC (clone AT-1, Stemcell Technologies), CD28-PE-Cy7 (clone CD28.2 ...
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bioRxiv - Immunology 2024Quote: ... were stained with anti-strep-tag II antibody (FITC) and enriched using the EasySep FITC Positive Selection Kit II according to the manufacturer’s instructions (StemCell Technologies) (Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
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bioRxiv - Cancer Biology 2024Quote: ... and Fms-like tyrosine kinase-3 Ligand (Flt3, StemCell Technologies, Cat#78009) at 25 ng/mL ...
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bioRxiv - Neuroscience 2021Quote: ... The FITC Selection Cocktail from the EasySep Human FITC Positive Selection Kit (STEMCELL Technologies) was added at a dilution of 1:10 and the cell suspension was incubated for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with specific neuronal marker CD24-FITC Clone M1/69 antibody(38) (Stemcell Technologies, Canada) diluted 1:100 for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... FITC-CD14 (MoP9, STEMCELL Technologies #60124FI).
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bioRxiv - Neuroscience 2023Quote: ... and CD11B-FITC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60040FI) antibodies for 15 minutes in the dark at 4C ...
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bioRxiv - Immunology 2021Quote: ... anti-CD38 FITC (Stem Cell technologies, #60131FI) at a 1:200 dilution and the live/dead marker Zombie NIR at a 1:400 dilution in FACS buffer.
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bioRxiv - Cell Biology 2023Quote: ... using FITC selection cocktail (18558; STEMCELL TECHNOLOGY), and isolated using MACS MS column (130-042-201 ...
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bioRxiv - Bioengineering 2020Quote: ... Fluorescence microscopy (Keyence Epi-Fluorescence microscope BZ-X710, Keyence, Japan) was performed using anti-dextran FITC antibody (Stemcell Technologies, Vancouver, Canada) co-incubated with T cells for 24 h in a chamber slide for live cell imaging ...
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bioRxiv - Cell Biology 2023Quote: iPSCs were differentiated into ventricular-like iPSC-CMs through temporal modulation of WNT signaling.15,18,19 iPSCs were treated with CHIR99021 (Stemcell Technologies) for 24 hrs (day 0-1 ...
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bioRxiv - Cancer Biology 2023Quote: The purity of cultured CTC subpopulation was validated by gating the sorted cells with conjugated primary antibodies of CD44-FITC (Stem Cell Technologies), CD24-PE ...
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bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... integrated and inducible pure human cortical-like glutamatergic neurons (i3Ns) was completed in two weeks in BrainPhys media (STEMCELL Technologies, 05790) (Figure ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
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bioRxiv - Immunology 2024Quote: ... FITC-conjugated mouse IgG2b anti-human CD32 Ab (clone IV.3, cat. no. 60012Fl.1, StemCell Technologies, Vancouver, BC, Canada). Mouse isotype controls APC-conjugated mouse IgG2a ...
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bioRxiv - Bioengineering 2023Quote: Day (d)25 immature β-like clusters were washed twice with PBS-and dissociated into a single-cell suspension with 8 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: Day 25 immature β-like clusters were washed twice with PBS-and dissociated into a single-cell suspension with 8 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: d25 immature β-like clusters were washed twice with PBS-and dissociated into a single-cell suspension with 8 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... anti-GYPA-FITC clone 2B7 mouse monoclonal (Stem Cell Tech 60152FI), anti-GYPC-FITC mouse monoclonal (IBGRL) ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Immunology 2022Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... and enriched using the EasySep FITC Positive Selection Kit II(StemCell Technologies) per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... all from BD Biosciences; anti-CD38-FITC (clone AT1; 2.5 μL; cat. # 60131FI) from STEMCELL Technologies; anti-CD161-BV421 (clone HP-3G10 ...
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bioRxiv - Cell Biology 2024Quote: ... The antibodies used are anti-TRA-1-60-AF488 (STEMCELL Technologies, 60064AD.1), anti-CXCR4-PE (BioLegend ...
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bioRxiv - Genetics 2022Quote: ... Anti-nestin mouse antibody (StemCell Technologies, 60091, 1:1000), Alexa Fluor 488-anti-Mouse (A11029 ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 μg/mL rabbit anti-Tight Junction Protein 1 (TJP1) (StemCell Technologies, 100-0750), 1 μg/mL mouse anti-acetylated alpha-Tubulin (StemCell Technologies ...
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bioRxiv - Genomics 2024Quote: ... The control antibody (0.2 µg/µl, 1:1134, STEMCELL Technologies, 60070AD) was added to the isotype control ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Antibodies used in immunofluorescence: Anti-Mouse OCT4 (STEMCELL TECHNOLOGIES, 60059, 1:200); Rabbit Monoclonal Anti-Sox9 (abcam ...
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bioRxiv - Neuroscience 2024Quote: ... the control antibody was added (0.2 µg/µl, 1:3390, STEMCELL Technologies, 60070AD). Antibodies were incubated for 10 min at 4 °C in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... Isotype control was stained using control antibody (STEMCELL Technologies, 60070AD.1, 0.2 ug/ul) at 1:378 dilution for 10 min at 4 °C protected from light ...
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bioRxiv - Bioengineering 2023Quote: ... The following primary antibodies were used: beta-tubulin III (STEMCELL Technologies 60052; 1:1000), GFAP (Dako GA524 ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Neuroscience 2020Quote: ... carrying genes for tdTomato and the murine cell-surface protein CD90.2/Thy1.2 driven by the endogenous POU4F2 (BRN3B) promoter were clonally propagated in mTeSR-1 media (StemCell Technologies, Cambridge, MA) on growth factor-reduced Matrigel substrate (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 nM bone morphogenetic protein 4 (BMP4, Stemcell technologies) was added to the medium for another three days ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were then stained for F4/80+ positivity by first using a F4/80-PE conjugated antibody (StemCell Technologies Cat# 60027PE.1) and incubated for 15 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... anti-SSEA-4 antibody (STEMCELL Technologies), anti-Sox17 antibody (R&D System) ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with antibodies against CD3/CD28 (2 µg/mL CD3 and 1 µg/mL CD28 antibodies, ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, or eBioscience) or PMA (20 ng/mL and ionomycin (1 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... matrix protein coated plates and cultured in mTeSR™ medium (STEMCELL tech), passaged using Versene (Gibco ...