Labshake search
Citations for Stemcell Technologies :
701 - 750 of 1280 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by immuno-magnetic negative selection using the EasySep Direct human neutrophil isolation kit (StemCell Technologies). A highly pure population of neutrophils was then generated by cell sorting on the basis of size ...
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bioRxiv - Neuroscience 2023Quote: ... hematopoietic lineage to obtain hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Catalog No. 05310). Next ...
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bioRxiv - Bioengineering 2024Quote: ... T-cells were then positively selected using an EasySep T-cell kit (Cat # 17951, STEMCELL Technologies, Vancouver, Canada) and an EasySep Magnet (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... mature microglial cells were isolated from astrocytes using EasySep Mouse CD11b Positive Selection Kit II (Stemcell Technologies #18970A,) and subsequently seeded into p60 plates ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies, 17961). Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were further lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... spleen-derived B-cells were selected using the EasySep™ Mouse Pan-B Cell Isolation Kit (STEMCELL Tech).
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Human CD34+ cells were magnetically enriched with the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies) from either normal BM or CB samples ...
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bioRxiv - Genetics 2024Quote: ... HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... total CD8+ T cells were first enriched using the EasySep™ Mouse CD8+ T cell isolation kit (Stemcell Technologies) and then CD8+CD44hiCD62Llo central memory T cells purified by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were isolated for intravenous injection using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies, Canada). 4×106 isolated T cells were injected intravenously per mouse.
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bioRxiv - Systems Biology 2020Quote: ... CD66b and glycophorin A were depleted from PBMCs using magnetic sorting (Human Pan-DC Pre-Enrichment Kit, StemCell Technologies). pDCs were then sorted on a FACS Vantage instrument (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Macrophages were separated from fibroblasts with the use of the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to phycoerythrin.
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bioRxiv - Neuroscience 2021Quote: ... Spinal cord isolation was performed using contents of ‘NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (StemCell Technologies)’ ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were isolated from PBMCs via the Easysep Human CD4+ T-cell negative enrichment kit (STEMCELL Technologies). The isolated human CD4+ T cells were cultured at 2.5 × 106 cells/ml in complete RPMI medium (cRPMI) ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from PBMCs with EasySep™ Human T cell Isolation Kit per manufacturer’s instructions (StemCell Technologies). Isolated T cells (0.5-1 × 105 per well ...
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bioRxiv - Cancer Biology 2019Quote: ... ALDH activity in the cells was measured by flow cytometry using the ALDEFLUOR kit (Stemcell technologies, Vancouver, BC, Canada). Cells with low and high levels of ALDH enzymatic activity (respectively ALDH- and ALDH+ cells ...
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bioRxiv - Cancer Biology 2019Quote: ... we evaluated tumor propagating cell numbers in hematopoietic malignancies using an ALDEFLUOR™ kit (StemCell Technologies, Vancouver, BC, CA) to detect ALDH1+ cells ...
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bioRxiv - Cancer Biology 2019Quote: ... These cells were grown in suspension in NeuroCultTM NS-A Proliferation Kit with proliferation supplement (StemCell Technologies, Cat 05751), 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF ...
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bioRxiv - Molecular Biology 2019Quote: ... Definitive endoderm (DE) was derived from a culture of H1 hESCs using the StemDiff Definitive Endoderm kit (StemCell Technologies).
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bioRxiv - Microbiology 2019Quote: ... B cells were isolated from PBMCs through negative enrichment using EasySep Human B cell isolation kit (Stem Cell Technologies) following manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 5×104/mL bone marrow and 5×105/mL spleen cells were cultured with MegaCult-C Kit (STEMCELL TECHNOLOGIES) for 9 days ...
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bioRxiv - Immunology 2021Quote: ... Poly I:C-activated NK cells were isolated from the spleen using EasySep Mouse NK Cell Isolation Kit (STEMCELL Technologies). Purified NK cells were co-cultured with target cells at a ratio of 5:1 ...
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bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
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bioRxiv - Bioengineering 2021Quote: ... Reprogramming of erythroid progenitor cells was performed using the Erythroid Progenitor Reprogramming Kit following manufacturer’s instructions (Stem Cell Technologies). Briefly ...
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bioRxiv - Neuroscience 2020Quote: Differentiation into cerebral organoids was performed with a commercially available differentiation kit from Stemcell Technologies (Catalog number 08570-1) based on previously reported protocols [8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
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bioRxiv - Immunology 2021Quote: ESC or iPSC were differentiated to hematopoietic progenitors following the manufacturer’s instructions for the STEMdiff Hematopoietic Kit (STEMCELL Technologies). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... The differentiation of neural progenitor cells (NPCs) was induced by STEMdiff SMADi Neural induction kit from STEM Cell Technologies. The differentiation of astrocytes was initiated using the Astrocyte medium (ScienCell ...
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bioRxiv - Immunology 2022Quote: ... White blood cells were isolated with the Erythroclear Red Blood Cell Depletion Reagent Kit (STEMCELL Technologies; cat. no. 01738).
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bioRxiv - Immunology 2022Quote: Isolation of neutrophils was carried out EasySep™ Direct Human Neutrophil Isolation Kit (Stem Cell Technologies, Vancouver, Canada, 19666), an immunomagnetic negative selection kit ...
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bioRxiv - Immunology 2020Quote: ... splenic NK cells were enriched to >80% purity using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies), and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified from thawed PBMCs using the EasySep Human T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Immunology 2022Quote: ... CD4+ Human T cells were enriched from PBMCs using EasySep™ Human CD4+ T Cell Isolation Kit (StemCell Technologies) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
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bioRxiv - Synthetic Biology 2019Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc., Vancouver, BC, Canada). The resulting CD34+ HSPCs were cultured in StemSpan SFEM II (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched from the SCS using Mouse Pan-B Cell Isolation kits (Stemcell Technologies, Vancouver, BC, Canada). Briefly ...
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bioRxiv - Immunology 2020Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II for future studies (Stemcell Technologies, #17877). The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit ...
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bioRxiv - Cell Biology 2019Quote: ... Human neutrophils were isolated within 1h after drawn using the human neutrophil direct isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Genetics 2021Quote: ... we performed immunohistochemistry in 11-week-old human cerebral organoids generated with the STEMdiff cerebral organoid kit (STEMCELL Technologies), and C57BL/6J mouse brains (Jackson laboratory ...
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bioRxiv - Immunology 2021Quote: ... Thy1.1+ cells were positively enriched using the EasySep™ PE Positive Selection Kit (Stem Cell Technologies, Cat no. 17684) or EasySep™ Release Mouse PE Positive Selection Kit (Stem Cell Technologies ...
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bioRxiv - Immunology 2020Quote: CD4+T-cells were isolated from PBMCs with the EasySep™ human CD4+T-cell enrichment kit (StemCell Technologies). T-cells/PBMCs were added to cultured hepatic epithelial cell lines (Huh-7 ...