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Citations for Stemcell Technologies :
401 - 450 of 495 citations for Mouse anti Marburg Virus FM11 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
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bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Immunology 2021Quote: ... Unlabeled anti-CD16/32 (clone 2.4G2; 60161) was purchased from STEMCELL Technologies. Data were acquired using an LSRFortessa (BD Biosciences ...
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bioRxiv - Developmental Biology 2020Quote: ... 6-well plates were pretreated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min incubation at room temperature followed by two rinses with equal volumes of fresh HBSS ...
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bioRxiv - Neuroscience 2019Quote: ... anti-human CD40 antibody (clone 5C3) and 20 ng rhIL4 (Stemcell Technologies) was added to culture medium ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Cell Biology 2022Quote: Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... pre-coated with Anti-adherence rinsing solution (StemCell Technologies cat. no. 07010) at 3 million cells per well for EB formation ...
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bioRxiv - Bioengineering 2022Quote: ... were pretreated with an anti-adhesion rinse solution (Stem Cell Technologies 07010) for a minimum of 15min to prevent cell attachment ...
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bioRxiv - Immunology 2022Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Neuroscience 2022Quote: ... AggreWells were pre-treated with Anti-adherence Rinsing Solution (Stem Cell Technologies) and AggreWell plates were centrifuged following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... was prepared by washing with anti-adherence solution (07010,Stem Cell Technologies). The aggrewell-800 plate is a 24-well culture plate each well contains 300 microwells used for the creation of EBs from hiPSCs ...
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bioRxiv - Cancer Biology 2023Quote: PDECs were treated with 25ul/mL anti-CD3/CD28/CD2 (Stemcell Technologies), 100ug/mL Atezolizumab (Selleck Chemicals) ...
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bioRxiv - Developmental Biology 2023Quote: ... was prepared by pre-coating with anti-adherance solution (07010, STEMCELL Technologies) and centrifuging at 2000g for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The spinner flasks were coated with anti-adherenet rinsing solution (StemCell technologies) before transferring the EBs ...
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bioRxiv - Developmental Biology 2024Quote: ... all plasticware were pre-wetted with Anti- Adherence Rinsing Solution (StemCell Technologies). Matrigel domes containing organoids were broken manually by pipetting up and down with cold DMEM/F-12 (Gibco) ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... followed by adding 0.5 ml of anti-adherence solution (Stemcell Technologies, 07010). Plates were centrifuged 5 minutes at 1,000 rcf and incubated 20 minutes at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... all from BD Biosciences; anti-CD38-FITC (clone AT1; 2.5 μL; cat. # 60131FI) from STEMCELL Technologies; anti-CD161-BV421 (clone HP-3G10 ...
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bioRxiv - Developmental Biology 2022Quote: ... Six-well plates were pre-treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min incubation at room temperature followed by two rinses with equal volumes of HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... embryos upon depletion of erythroid cells using anti-Ter119 immunomagnetic microbeads (StemCell Technologies). Neonatal CD34+ HSPCs were obtained from cord blood of healthy donors and enriched using anti-CD34 immunomagnetic microbeads (Miltenyi Biotech and StemCell Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with 1:25 anti-human CD45 FITC (clone 2D1, STEMCELL Technologies), 1:50 anti-human CD45 APC-ef780 (clone HI30 ...
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bioRxiv - Developmental Biology 2022Quote: ... Each well was precoated with 500µl of Anti-Adherence Rinsing Solution (Stemcell technologies) and spun for 5 minutes at 1500 rcf ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 cm dishes were coated with Anti-adherence rinsing solution (Stemcell technologies, 7010) during 2 min ...
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bioRxiv - Microbiology 2023Quote: ... Tissue culture dishes were treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies™). EBs were cultured in expansion medium until day 25 with medium changes every 2-3 days ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Microbiology 2020Quote: ... Anti-CD3/CD28 antibody-coated beads (ImmunoCult) were purchased from STEMCELL technologies (Vancouver, CA).
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bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
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bioRxiv - Cell Biology 2021Quote: A volume of 50 µl of Anti-Adherence Rinsing Solution (STEMCELL Technologies, Cambridge, UK) was added to each well of a clear green coded 96-well round base plate for suspension (Sarstedt ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Immunology 2020Quote: ... were prepared by addition of 0.5 mL Anti-Adherence Rinsing solution (Stemcell technologies, #07010) and centrifugation at 3000g for 3 minutes to remove bubbles from the microwells ...
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bioRxiv - Genetics 2019Quote: ... antibodies used were Anti-Beta-Tubulin III Antibody Clone TUJ1 (STEMCELL Technologies, BC, Canada) and Texas Red dye-conjugated AffiniPure Goat Anti-Mouse IgG (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... The microwells were first coated with an anti-adherence rinsing solution (07010, StemCell Technologies) and centrifuged at 2,000 g for 5 min ...