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Citations for Stemcell Technologies :
501 - 550 of 4682 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies). CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Molecular Biology 2022Quote: ... were purified with the EasySep Mouse Hematopoietic Progenitor Cell Isolation kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and the EasySep Mouse NK Cell Isolation Kit (StemCell Technologies, cat no. 19855) was used to isolate NK cells through negative selection ...
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bioRxiv - Immunology 2020Quote: ... splenic NK cells were enriched to >80% purity using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies), and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... and lineage negative cell fraction were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies #19856). Cell sorting of LSK or LT-HSC ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology, # 10981) and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology ...
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bioRxiv - Bioengineering 2022Quote: ... Alpha MEM with nucleosides (StemCell Technologies, Vancouver, Canada) were purchased for cell culture purposes ...
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bioRxiv - Cancer Biology 2021Quote: ... and activated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Stem Cell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CD4 T cells (non-irradiated) were isolated by magnetic bead selection (Cell Stemcell Technologies). Cells were cultured for 72 hours and flow cytometric analysis used to assess proliferation as measured by CFSE dilution.
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bioRxiv - Cancer Biology 2022Quote: Primary T cells were isolated from LeukoPaks obtained from Stem Cell Technologies (200-0092). CD8 and CD4 cells were isolated separately using their EasySep™ Human CD8+/CD4+ T Cell Isolation Kit as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated CD4+ T cells were then subjected to a CD25+ cell isolation (STEMCELl Technologies) using releasable magnetic spheres (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Immunology 2019Quote: ... and NK cells were purified by negative selection using EasySep Mouse NK Cell Isolation Kit (STEMCELL TECHNOLOGIES). Normalized NK-cell numbers (1–3 × 106 ...
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bioRxiv - Immunology 2022Quote: NK cells from the spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Pretumor splenic B cells were purified using the Mouse Pan-B Cell Isolation Kit by StemCell Technologies. Bone marrow cells were flushed from femurs and tibia ...
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bioRxiv - Immunology 2021Quote: ... Resting B cells were isolated using the EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies), which depletes for non-B cell markers and activated CD43+ B cells ...
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bioRxiv - Immunology 2022Quote: ... and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855) from tumor-free wild type B6 mice in the indicated ratios in the presence of IL-2 as described previously (72) ...
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bioRxiv - Immunology 2021Quote: ... Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 24 mM [U-13C]glucose ...
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bioRxiv - Bioengineering 2021Quote: Pan T cells were isolated from donor PBMC’s (#70025, StemCell Technologies) using EasySep™ human T cell isolation kit (#17951 ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured in ImmunoCult-XF T cell Expansion Medium (StemCell Technologies). Approximately 24 hours post-isolation ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using negative selection (STEMCELL Technologies #17952) and were immediately cultured in complete RPMI media (RPMI 1640 (Gibco #22400089 ...
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bioRxiv - Immunology 2024Quote: Pan T cells were isolated (Miltenyi) from PBMCs (Leukopak, StemCell Technologies) and plated in TexMACS or ImmunoCult at 7x105 cells/ml in a 6-well plate ...
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bioRxiv - Genomics 2024Quote: CD3+ T cells were isolated from healthy donor leukopaks (Stemcell Technologies) using bead separation (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... CD3+ T cells were isolated by magnetic bead separation (StemCell Technology) from PBMCs of three leukopaks (Cambridge Biosciences ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... ImmunoCult™ human CD3/CD28/CD2 T-cell activator (StemCell Technologies) was used to activate isolated T cells in vitro for 2 days following the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2024Quote: ... ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada), CD3Ab OKT3 (Sino Biological ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were suspended in CryoStor 10 (StemCell Technologies, Seattle, WA), aliquoted ...
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bioRxiv - Immunology 2019Quote: OT1 and OTII T cells were isolated from LN of TCR transgenic mice using either a CD8 or CD4 StemSep enrichment kit (STEMCELL Technologies), respectively ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: Primary human CD4+ T cells were isolated from PBMCs using a Human resting CD4+ T Cells Isolation Kit I according to the manufacturer instructions (STEMCELL Technologies). Briefly ...
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bioRxiv - Immunology 2019Quote: ... cryopreserved PBMCs were thawed (as above) and CD4 T cells were isolated by negative selection using the EasySep Human CD4 Enrichment Kit (Stemcell Technologies) before DNA extraction with the QiaAMP Blood Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
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bioRxiv - Immunology 2020Quote: ... were isolated from perioperative human patients’ brain tissues (Nervous System Tumor Bank) using EasySep Human CD8 T Cells Isolation Kit (STEMCELL Technologies) following the tissue digestion as previously described [68 ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Genomics 2023Quote: Human CD8+ T cells were obtained from either pooled PBMC donors (ZenBio) using negative selection human CD8 isolation kits (StemCell Technologies) or directly from vials containing isolated CD8+ T cells from individual donors (StemCell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated by negative selection using the EasySep Human CD8 negative isolation kit (StemCell Technologies, Vancouver, BC).
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bioRxiv - Immunology 2023Quote: ... ETRAMP-specific CD4+ T cells were enriched using APC- and PE-conjugated tetramers and EasySep APC and PE Positive Selection Kits (STEMCELL Technologies). Cells were then stained with fluorescent dye-conjugated antibodies (see Table I ...
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bioRxiv - Cell Biology 2024Quote: ... and c-Kit/CD117 antibodies (STEMCELL Technologies) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... all the unwanted cells were labelled with magnet conjugated antibody cocktail which is separated using their EasySep magnetic stand leaving CD4 or CD8 cell in suspension using the vendor supplied EasySep Human CD4/CD8 T Cell Iso Kit (Stem Cell Technologies, 17952 for CD4 and 17953 for CD8). This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals ...
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bioRxiv - Immunology 2019Quote: HSPCs were isolated using Easy Sep Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell Technologies) according to manufactures’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Frozen cells were thawed at 37°C and treated using the ErythroClear™ Red Blood Cell Depletion Kit (STEMCELL Technologies). Cells were constructed into barcoded single-cell cDNA libraries and sequenced as described above.
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bioRxiv - Bioengineering 2020Quote: ... CD8+ cells were isolated using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: Human Peripheral Blood Pan-T Cells was purchased from Stem Cell Technologies (Vancouver, BC, Canada) and Panc-1 cells were obtained from the Kansas University Medical Center ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...