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Citations for Stemcell Technologies :
301 - 350 of 2344 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2020Quote: ... from individual mice were pooled and pre-enriched for CD4 T cells using EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies). Live ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse neural stem cells (NSCs) were expanded in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse glioma cells (CPEvIII) were cultured in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Growth media was NeuroCult NSC Basal Medium (Mouse) containing NeuroCult Proliferation Supplement (Mouse) (StemCell Technologies), 0.2% heparin solution (Stem-Cell Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in mTeSR1 + 1% P/S and 10 µM Rock Inhibitor (Y-27632, StemCell Technologies, #72308). A full detailed protocol for cell line maintenance can be found at www.allencell.org/sops SOP ...
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bioRxiv - Neuroscience 2020Quote: ... and passed weekly at a ratio of 1:10-50 dependent on colony confluency using ReLeSR (StemCell Technologies).
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2019Quote: ... Initial HSPC isolation was performed using defined protocols of EasySep™ Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (#19756, #18000, Stemcell Technologies, CA). A secondary isolation further enriched the HSPC population by collecting the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Cancer Biology 2020Quote: ... from wild-type or Jak1 floxed C57/Bl6 mice were isolated using EasySep mouse hematopoietic progenitor cell isolation kit (Stemcell technologies, Vancouver, Canada) which remove lineage marker-expressing cells with biotinylated antibodies directed against non-hematopoietic stem cells and non-progenitor cells (CD5 ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
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bioRxiv - Immunology 2024Quote: ... were cultured with lymphocytes (2.5 × 104) magnetically sorted frommousespleen singlecell preparations using MACs columns and the EasySep™ Mouse TCell Isolation Kit (StemCell Technologies Cat# 19851) for a total of 6d with 20 units/mL of IL-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco], 10% BIT 9500 [StemCell Technologies], and 1 mM glutamine [Gibco]) supplemented with 20ng/ml EGF (Chemicon) ...
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bioRxiv - Cancer Biology 2022Quote: ... was cultured in standard mouse NSC media containing NeuroCult basal medium (mouse & rat, STEMCELL Technologies, #05700) supplemented with NeuroCult proliferation kit (mouse & rat ...
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bioRxiv - Developmental Biology 2022Quote: ... 10% CloneR (StemCell Technologies), and 1 μM Pifithrin-α hydrobromide (Tocris) ...
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bioRxiv - Bioengineering 2023Quote: The Mouse IntestiCult medium (STEMCELL Technologies) was prepared and supplemented with 100 U/mL Pen/Strep (Gibco) ...
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bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...