Labshake search
Citations for Stemcell Technologies :
301 - 350 of 1569 citations for Mouse Fibroblast Growth Factor 15 FGF15 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... then passage into a primary methylcellulose-based medium containing murine Stem Cell Factor (mSCF) following the manufacturer’s protocol (STEMCELL Technologies), where they differentiate into pluripotent ...
-
bioRxiv - Bioengineering 2020Quote: ... and in the presence of the commercially available medium IntestiCult™ Organoid Growth Medium (Human) (STEMCELL Technologies, 06010), supplemented with the antibiotic primocin (Invivogen ...
-
bioRxiv - Genomics 2021Quote: ... 25 000 cells were sorted into IntestiCult Organoid Growth Medium (0600-0, −2 and −3 combined, STEMCELL Technologies), centrifuged and directly processed for library generation with the 10x Genomics technology according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... and were maintained at ALI for 21+ days in a Pneumacult™ ALI growth medium (Stem Cell Technologies) at 37°C/5% CO2 in a humidified incubator ...
-
bioRxiv - Microbiology 2022Quote: ... The sheared enteroids were then diluted to the appropriate concentration with IntestiCult™ Organoid Growth Medium (STEMCELL Technologies) supplemented with 10μM ROCK pathway inhibitor (Cayman chemicals) ...
-
An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... according to the protocol with SepMate - 15 tubes (cat. no. 85415, StemCell Technologies) and Lymphoprep (cat ...
-
bioRxiv - Cancer Biology 2021Quote: ... naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) and stimulated with plate-bound α-CD3 (5 μg/ml ...
-
bioRxiv - Cancer Biology 2021Quote: Naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Bone marrow neutrophils were collected from the femur and tibia of naïve WT C57BL/6J and MPO-deficient mice using the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). 2×105 neutrophils were plated in Hank’s Balanced Salt Solution (HBSS ...
-
bioRxiv - Genomics 2020Quote: ... CD4 and CD8 T cells were isolated from a mouse using CD4 and CD8 negative selection kits respectively (StemCell Technologies) and were labelled with the caged strand hybridized to the Ab-oligo conjugate ...
-
bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
-
bioRxiv - Bioengineering 2020Quote: ... Initial enrichment for HSPC was performed with the EasySep Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (Stemcell Technologies, CA). Further isolation was performed to obtain a population of the Lin− Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.1mM EDTA-2Na).Lineage negative (Lin−) cells were enriched using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell technologies) and stained with fluorescent antibodies ...
-
bioRxiv - Immunology 2020Quote: ... CD3+ T cells were isolated from whole splenocytes using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies, #19851), as per the manufacturer’s guidelines ...
-
bioRxiv - Bioengineering 2021Quote: ... Isolation of hematopoietic lineage negative was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA). For cytotoxicity studies ...
-
bioRxiv - Immunology 2020Quote: CD8 T cells were purified from spleens by negative selection using EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
-
bioRxiv - Bioengineering 2020Quote: ... Initial hematopoietic lineage negative enrichment was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA), followed by collection of the Lin- Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Immunology 2022Quote: ... Lm-OVA-specific CD8+ OT-I T cells were isolated from the spleen of infected mice by positive selection using the EasySep mouse CD90.1 positive selection kit (StemCell Technologies) as previously described (2 ...
-
bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
-
bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
-
bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
-
bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
-
bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
-
bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
-
bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
-
bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse organoid culture: we used cryopreserved mouse intestinal organoids (70931, STEMCELL Technologies) derived from the C57BL/6 mouse small intestine ...
-
bioRxiv - Neuroscience 2023Quote: ... Matrigel was diluted according to the lot-specific dilution factor recommended by the manufacturer in 25 ml of DMEM/F12 HEPES (Cat No. 36254, STEMCELL Technologies) and set for 1 hour at 37°C before plates were used ...
-
bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
-
bioRxiv - Immunology 2021Quote: ... The selected cloned cell lines secreting mAb H5.28 or H5.31 were grown initially in hybridoma growth medium (ClonaCell-HY medium E from STEMCELL Technologies). Prior to antibody expression and purification ...
-
bioRxiv - Developmental Biology 2023Quote: ... Mammary organoids from eutherian species were cultured in Intesticult human organoid growth media (StemCell Technologies, Vancouver, Canada, Cat# 06010). Mammary organoids of opossum were cultured in either Intesticult ...
-
bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
-
bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
-
Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
-
bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
-
bioRxiv - Cancer Biology 2019Quote: Mouse CD3 positive T cells were isolated from spleens collected from CEA transgenic mice using negative selection kit (Stemcell Technologies, Canada) and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend ...
-
bioRxiv - Cell Biology 2021Quote: ... Magnetic depletion of lineage-positive haematopoietic cells was performed using the EasySep mouse haematopoietic progenitor cell enrichment kit (Stem Cell Technologies), and lineage-depleted stem and progenitor cells were submitted to mass spectrometry analysis ...
-
bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
-
bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Splenocytes and lung cells were enriched for total B cells using the EasySep Mouse Pan-B Cell Isolation kit (Stemcell Technologies) while whole mln cells were used for downstream processing ...
-
bioRxiv - Cell Biology 2020Quote: ... Adipogenic or osteogenic differentiation of MEFs was induced for 28 days with MesenCult™ Adipogenic Differentiation Kit (Mouse) (STEMCELL Technologies, Canada) and MesenCult™ Osteogenic Stimulatory Kit (Mouse) ...
-
bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens or mesenteric lymph nodes (MLNs) using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). Where indicated ...