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Citations for Stemcell Technologies :
1 - 50 of 1992 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 p17 1981 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... HIV-negative donors using the RosetteSep Human CD4 T cell enrichment kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Nestin (STEMCELL Technologies; 1:100); Rabbit anti-GFAP (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Beta Tubulin (STEMCELL Technologies; 1:500); Rabbit anti-Olig2 (PhosphoSolutions ...
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bioRxiv - Genetics 2022Quote: ... Anti-nestin mouse antibody (StemCell Technologies, 60091, 1:1000), Alexa Fluor 488-anti-Mouse (A11029 ...
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bioRxiv - Cancer Biology 2022Quote: ... human leukemia cells were isolated using EasySepTM Mouse/Human Chimera Isolation Kit (#19849; Stemcell Technologies). All animal experiments were approved by and performed in accordance with guidelines of the Institutional Animal Care and Use Committee at University of Colorado (protocol No ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with 1:25 anti-human CD45 FITC (clone 2D1, STEMCELL Technologies), 1:50 anti-human CD45 APC-ef780 (clone HI30 ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Genomics 2021Quote: ... Antibodies used in immunofluorescence: Anti-Mouse OCT4 (STEMCELL TECHNOLOGIES, 60059, 1:200); Rabbit Monoclonal Anti-Sox9 (abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... or anti-human CD32 (FcγRII Blocker, StemCell Technologies) for 15 minutes on ice to block non-specific staining and then incubated with fluorochrome-conjugated antibodies for 30 minutes on ice and protected from light ...
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bioRxiv - Molecular Biology 2022Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using Lymphoprep™ and SepMate™ tubes (StemCell Technologies), and cryopreserved [41] ...
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bioRxiv - Immunology 2024Quote: ... 1.5 µg/mL anti-human CD32 antibody (STEMCELL Technologies) and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2021Quote: ... we respectively used mouse- and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Immunology 2023Quote: ... we used mouse-specific and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Neuroscience 2019Quote: ... anti-human CD40 antibody (clone 5C3) and 20 ng rhIL4 (Stemcell Technologies) was added to culture medium ...
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bioRxiv - Cell Biology 2022Quote: Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Human T cells were also isolated from the thymus tissue in humanized SRG rats via immunomagnetic selection using anti-human CD3 antibody (EasySep™ Human CD3 Positive Selection, Catalog # 17951, Stemcell Technologies) and treated without (vehicle ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Leukemia Inhibitory Factor (LIF, STEMCELL Technologies, 78055.1) and 10 ng/mL human recombinant Epidermal Growth Factor (EGF ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Leukemia Inhibitory Factor (LIF, STEMCELL Technologies, 78055.1) and 10 ng/mL human recombinant Epidermal Growth Factor (EGF ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng μL-1 recombinant human IL-2 (StemCell Technologies) and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
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bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Microbiology 2023Quote: ... anti-GYPA-FITC clone 2B7 mouse monoclonal (Stem Cell Tech 60152FI), anti-GYPC-FITC mouse monoclonal (IBGRL) ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies, Catalog #60030BT) according to the manufacturers protocol.
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bioRxiv - Cell Biology 2020Quote: ... and the following mouse monoclonal antibodies: anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) and anti-Ku80 (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed) (STEMCELL Technologies) for 1 hour on ice ...