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Citations for Stemcell Technologies :
251 - 300 of 642 citations for Mouse Anti Hepatitis C Virus NS5b Antibody 1825 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Vials of cryopreserved PBMCs were thawed at 37°C and washed in ClonaCell™-HY Medium A (STEMCELL Technologies, cat. #03801). Epstein-Barr virus (EBV ...
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bioRxiv - Genetics 2022Quote: ... Human ESCs (wild type and ADAR1 KO, gifts from Dr. C. Rice, the Rockefeller University) were maintained in complete mTeSR1 (StemCell Technologies) and the differentiated neural progenitor cells were maintained in STEMdiff Neural Progenitor Medium (StemCell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with Dulbecco’s Modified Eagle Medium (DMEM/F12) and incubated for 70 min (37°C, 5 % CO2) in STEMdiff Neural Rosette Selection Reagent (StemCell Technologies). Rosettes were lifted by gently dispensing DMEM/F12 onto the colonies ...
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bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
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bioRxiv - Molecular Biology 2023Quote: ... HSPCs derived from healthy donors or αTM patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies) as previously described14,38 ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were maintained in a 37°C/5% CO2 incubator and cells were fed every four days with neural maturation medium (BrainPhys plus SM1 supplement (Stemcell Technologies) supplemented with 10ng/ml of BDNF ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Neurons were grown at 37°C/5% CO2 and fed via half-media exchange every 3rd day with astrocyte conditioned BrainPhys media (StemCell Technologies) supplemented with 1% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... GBOs cultured for more than one month were dissociated into a single cell with accutase at 37 °C for 30 min and seeded into aggrewell 800 (Stemcell technologies) at a density of 9 × 105 cells/mL with 1 mL of GBO medium supplemented with 10 µM Y27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Immunology 2019Quote: ... using antibody-coated magnetic beads with a RoboSep instrument (StemCell Technologies). Purified CD4+ T-cells were stimulated for 3 days with concanavalin A (5μg/mL ...
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bioRxiv - Genetics 2021Quote: ... ∼4 × 105 CS iPSCs containing a c.1569G>A (p.(W523X)) mutation were treated with Accutase (working dilution 1:3 in PBS; StemCell Technologies #07922,). Cells were dissociated to generate a suspension of single cells ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 5 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium containing hematopoietic cells and trypsinized cells were collected for FACS analysis and CFU-C assay in Methylcellulose M3434 (Stem Cell Technologies). After 10 days of culture at 37°C in 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2021Quote: ... isolated T-IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
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bioRxiv - Immunology 2019Quote: ... hMT and s-hMT mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Genomics 2020Quote: ... and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Immunology 2019Quote: ... and NK cells were purified by negative selection using EasySep Mouse NK Cell Isolation Kit (STEMCELL TECHNOLOGIES). Normalized NK-cell numbers (1–3 × 106 ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from disaggregated synovium with the EasySep Mouse T cell isolation kit (STEMCELL Tech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were purified by negative selection with the EasySep Mouse T cell isolation kit (STEMCELL Tech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: NK cells from the spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Our murine neurosphere media consisted of 45 ml Neurocult Mouse Basal media (Stem Cell Technologies, Catalog# 05700), 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... negative selection using the EasySep Mouse T Cell or CD8+ T cell Isolation Kit (STEMCELL Technologies #19853) was used to isolate CD8+ T cells ...
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bioRxiv - Cell Biology 2022Quote: ... mouse embryonic fibroblasts (MEF) and HeLa cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) (Stemcell technologies) supplemented with 10% fetal bovine serum (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... T cell isolation was performed using EasySep mouse negative T cell isolation kit (Stemcell technologies, Cat # 19851) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: Intestinal crypts were dissociated from mouse small intestine using Gentle Cell Dissociation Reagent (Stemcell Technologies, Cambridge, UK). The crypts were then re-suspended in Intesticult medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: Pretumor splenic B cells were purified using the Mouse Pan-B Cell Isolation Kit by StemCell Technologies. Bone marrow cells were flushed from femurs and tibia ...
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and CD8+ T cells were negatively selected using EasySep mouse CD8+ T cells isolation kit (StemCell Technologies), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Resting B cells were isolated using the EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies), which depletes for non-B cell markers and activated CD43+ B cells ...
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bioRxiv - Immunology 2022Quote: ... and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855) from tumor-free wild type B6 mice in the indicated ratios in the presence of IL-2 as described previously (72) ...
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bioRxiv - Immunology 2022Quote: Bone-marrow eosinophils were isolated using EasySep™ Mouse Biotin Positive Selection Kit II (Stem Cell Technologies) according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: Mouse jejunal section were used to isolate crypts according to the manufacturer’s instructions (STEMCELL Technologies, document 28223). Complete IntestiCult medium was exchanged every 2-3 days and organoid numbers and de novo crypts were scored on days 5 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... All sorted cells were grown in Mouse MesenCult Expansion Kit Media (05513; StemCell Technologies, Vancouver, BC, Canada). Cells were cultured at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The mouse MLL-AF9-transduced cells were cultured in MethoCult™ M3234 (STEMCELL Technologies, Bancouber, BC, Canada). The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse intestinal organoids were cultured in 24-well plates using IntestiCult organoid growth medium (06005, STEMCELL Technologies). In short ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes or BM cells were enriched using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mouse myeloid cells were differentiated in IMDM (Cytiva, SH30228.FS) supplemented with 20% BIT9500 (StemCell Tech, 09500), 20 ng/mL M-CSF (Peprotech ...