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Citations for Stemcell Technologies :
451 - 500 of 536 citations for Mouse Anti Cholera Toxin Beta Subunit Antibody B8 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2021Quote: ... Unlabeled anti-CD16/32 (clone 2.4G2; 60161) was purchased from STEMCELL Technologies. Data were acquired using an LSRFortessa (BD Biosciences ...
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bioRxiv - Developmental Biology 2020Quote: ... 6-well plates were pretreated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min incubation at room temperature followed by two rinses with equal volumes of fresh HBSS ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Cell Biology 2022Quote: Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... pre-coated with Anti-adherence rinsing solution (StemCell Technologies cat. no. 07010) at 3 million cells per well for EB formation ...
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bioRxiv - Bioengineering 2022Quote: ... were pretreated with an anti-adhesion rinse solution (Stem Cell Technologies 07010) for a minimum of 15min to prevent cell attachment ...
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bioRxiv - Immunology 2022Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Neuroscience 2022Quote: ... AggreWells were pre-treated with Anti-adherence Rinsing Solution (Stem Cell Technologies) and AggreWell plates were centrifuged following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... was prepared by washing with anti-adherence solution (07010,Stem Cell Technologies). The aggrewell-800 plate is a 24-well culture plate each well contains 300 microwells used for the creation of EBs from hiPSCs ...
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bioRxiv - Developmental Biology 2023Quote: ... was prepared by pre-coating with anti-adherance solution (07010, STEMCELL Technologies) and centrifuging at 2000g for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: PDECs were treated with 25ul/mL anti-CD3/CD28/CD2 (Stemcell Technologies), 100ug/mL Atezolizumab (Selleck Chemicals) ...
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bioRxiv - Neuroscience 2023Quote: ... The spinner flasks were coated with anti-adherenet rinsing solution (StemCell technologies) before transferring the EBs ...
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bioRxiv - Developmental Biology 2024Quote: ... all plasticware were pre-wetted with Anti- Adherence Rinsing Solution (StemCell Technologies). Matrigel domes containing organoids were broken manually by pipetting up and down with cold DMEM/F-12 (Gibco) ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... followed by adding 0.5 ml of anti-adherence solution (Stemcell Technologies, 07010). Plates were centrifuged 5 minutes at 1,000 rcf and incubated 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with specific neuronal marker CD24-FITC Clone M1/69 antibody(38) (Stemcell Technologies, Canada) diluted 1:100 for 30 minutes on ice ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... all from BD Biosciences; anti-CD38-FITC (clone AT1; 2.5 μL; cat. # 60131FI) from STEMCELL Technologies; anti-CD161-BV421 (clone HP-3G10 ...
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bioRxiv - Developmental Biology 2022Quote: ... Six-well plates were pre-treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min incubation at room temperature followed by two rinses with equal volumes of HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... embryos upon depletion of erythroid cells using anti-Ter119 immunomagnetic microbeads (StemCell Technologies). Neonatal CD34+ HSPCs were obtained from cord blood of healthy donors and enriched using anti-CD34 immunomagnetic microbeads (Miltenyi Biotech and StemCell Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with 1:25 anti-human CD45 FITC (clone 2D1, STEMCELL Technologies), 1:50 anti-human CD45 APC-ef780 (clone HI30 ...
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bioRxiv - Developmental Biology 2022Quote: ... Each well was precoated with 500µl of Anti-Adherence Rinsing Solution (Stemcell technologies) and spun for 5 minutes at 1500 rcf ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 cm dishes were coated with Anti-adherence rinsing solution (Stemcell technologies, 7010) during 2 min ...
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bioRxiv - Microbiology 2023Quote: ... Tissue culture dishes were treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies™). EBs were cultured in expansion medium until day 25 with medium changes every 2-3 days ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Antibody-bound cells were depleted with streptavidin RapidSphere™ magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies; Vancouver, Canada). Cells with >90% purity were used for experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
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bioRxiv - Cell Biology 2021Quote: A volume of 50 µl of Anti-Adherence Rinsing Solution (STEMCELL Technologies, Cambridge, UK) was added to each well of a clear green coded 96-well round base plate for suspension (Sarstedt ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Immunology 2020Quote: ... were prepared by addition of 0.5 mL Anti-Adherence Rinsing solution (Stemcell technologies, #07010) and centrifugation at 3000g for 3 minutes to remove bubbles from the microwells ...
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bioRxiv - Neuroscience 2022Quote: ... The microwells were first coated with an anti-adherence rinsing solution (07010, StemCell Technologies) and centrifuged at 2,000 g for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 15 ml Falcon Tubes were rinsed with Anti-adherence Rinsing Solution (StemCell Technology, #07010) to prevent cell adhesion to the tube ...
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bioRxiv - Developmental Biology 2023Quote: ... 6-well plates were coated using an anti-adherence rinsing solution (Stem Cell Technologies) for 30min at 37℃ to make the low-attachment plates ...
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bioRxiv - Neuroscience 2023Quote: ... was prepared by centrifuging 500 µL Anti-Adherence Rinsing Solution (Stem Cell Technologies, 07010) at 1300 xg for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with a cocktail of lineage specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; Stemcell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... and an “activated” population that was cultured in medium supplemented with 25 μl/ml tetrameric antibody against CD2/CD3/CD28 (StemCell Technologies). Quiescent and activated T cell populations were cultured separately for 48 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Immunology 2022Quote: ... was prepared by addition of 0.5ml of Anti-Adherence Rinsing Solution (STEMCELL Technologies, no 07010) and centrifuged at 3,000 x g for three minutes to remove bubbles from the microwells ...