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Citations for Stemcell Technologies :
151 - 200 of 1004 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Primary human T cells were isolated from healthy donors using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies). Buffy coats were purchased from Stanford Blood Center and processed according to the manufacturer’s protocol using Lymphoprep density gradient medium and SepMate-50 tubes ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were obtained from PBMCs using the EasySep human CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France). Next ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were isolated from whole splenocytes using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies, #19851), as per the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... total CD4+ T cells were isolated using a combination of the human CD4+ T cell RosetteSep™ kit (STEMCELL Technologies) and Ficoll-Paque (Cytiva Inc. ...
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bioRxiv - Immunology 2020Quote: CD8 T cells were purified from spleens by negative selection using EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... CD8+ T cells were isolated from freshly-thawed PBMCs using the human CD8+ T cell isolation kit (Stem Cell Technologies). Anti-ILT3 52B8 (1µg/mL ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were isolated by negative selection with the EasySep Human Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified from non-adherent cells using Human Resting CD4+ T cell Isolation Kit (STEMCELL Technologies, 17962). A specific reagent to isolate resting T cells was selected to avoid the presence of pre-activated CD4+ T cells in sequencing samples ...
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bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
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bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cryopreserved in CryoStor10 (STEMCELL technologies). After thawing ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T-cell Isolation Kit (StemCell Technologies #19851 ...
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bioRxiv - Biochemistry 2023Quote: Human peripheral blood Pan-T cells (Stemcell Technologies) were thawed and expanded following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and passaged using T ReLeSR (Stem Cell Technologies). Quality control was performed with karyotyping (G-banding ...
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Immunology 2019Quote: ... T cells were extracted from spleen and CD4+ T cells were then purified using EasySep immunomagnetic negative selection (Stem Cell Technologies). Cells were then activated on plates as mentioned ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Immunology 2021Quote: ... Primary CD4+ or CD8+ T cells were isolated using EasySep Human CD4+ or CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... and LN cells were enriched for CD4+ T cells using the EasySep NHP CD4+ T cell isolation kit from STEMCELL Technologies Canada Inc (Cat# ...
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bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Microbiology 2020Quote: A lentiviral vector encoding anti-HIV-1 CAR was transduced and expressed in the enriched total human T cells obtained from fresh peripheral blood mononuclear cells by negative selection (EasySep Human T cell isolation kit, Stemcell Technologies). CAR-T cells were maintained in IMDM supplemented with 20% FBS ...
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bioRxiv - Microbiology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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bioRxiv - Microbiology 2021Quote: ... total CD4+ T cells were isolated from PBMCs by negative magnetic separation using EasySep Human CD4+ T cell isolation kit (StemCell Technology). Following treatment for 5 days (Fig ...
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bioRxiv - Immunology 2019Quote: ... naïve CD44+ T cells were enriched by magnetic separation using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies). 55×104 T cells were cultured for 6 days in the presence of plate-bound αCD3ε (5 μg mL−1) ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve CD4+ T cells were then enriched from total CD4+ T cells through immuno-magnetic negative selection with the EasySep™ Naïve CD4+ T cell isolation kit (STEMCELL Technologies). Naïve CD4+ T cell post-isolation purity (CD4+CD45RA+CD45RO- ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were first enriched for OT-II CD4+ T cells using EasySep™ mouse CD4+ T cell isolation kit (StemCell Technologies), incubated with FcR block and stained with the following fluorescence-conjugated antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and CD4+ T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s instructions and purity of isolated CD4+ T-cells was controlled on a FACSverse™ flow cytometer (BD Biosciences ...