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Citations for Stemcell Technologies :
551 - 600 of 1004 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... CD4 and CD8 T cells were isolated from a mouse using CD4 and CD8 negative selection kits respectively (StemCell Technologies) and were labelled with the caged strand hybridized to the Ab-oligo conjugate ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies). CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies) ...
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bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
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bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads according to the manufacturer’s instructions (StemCell Technologies). The cells were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2023Quote: OVA-specific CD8+ T cells were isolated from the spleens and lymph nodes of OTI/Thy1.1 mice by negative selection (Stemcell Technologies) and labeled with CFSE for proliferation tracking ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Immunology 2023Quote: ... 2D2 and OT-I cells were isolated respectively with magnetic CD4+ and CD8+ T cell selection beads (EasySep, STEMCELL Technologies).
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC, Canada). Isolated naïve CD8+ cells (1 x 106 cells/mL ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Immunology 2024Quote: ... Tregs were seeded at an approximate density of 1.2 million cells/cm2 in ImmunoCult-XF T cell expansion medium (STEMCELL Technologies) supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Cell Biology 2020Quote: ... The media was removed from the pellet and 200 ⎧l Accumax (STEMCELL technologies 07921) was added and incubated for 20 mins at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 5.5 mM L-cysteine HCl) and 125 units/mL DNase I (07900, Stemcell technologies) in Hanks’ Balanced Salt Solution (HBSS ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μg/ml Heparin (STEMCELL Technologies), 20 ng/ml bFGF (Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/ml heparin (StemCell Technologies), epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2020Quote: ... 2% NeuroCult SM1 supplement (STEMCELL Technologies), 2 mM GlutaMax.
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/mL FGF-2 (StemCell Technology), and 5nM Rock inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 µM SB431542 (StemCell Technologies). Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 2% SM1 (Stemcell Technologies, Vancouver, Canada), and 5 μM cytosine β-d-arabinofuranoside (AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μg/ml heparin (Stemcell Technologies), 50 units/ml penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... B27 (2%v/v, Stemcell technologies), N2 (0.5%v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 2% NeuroCult SM1 (05711, Stemcell technologies), 1% N2 Supplement (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Primary CD4+ T cells were purified from the blood of healthy donors by negative selection using the Human CD4+ T Cell Enrichment cocktail (StemCell Technologies). These primary cells were cultured in complete RPMI 1640 medium.
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bioRxiv - Immunology 2019Quote: OT1 and OTII T cells were isolated from LN of TCR transgenic mice using either a CD8 or CD4 StemSep enrichment kit (STEMCELL Technologies), respectively ...
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bioRxiv - Cancer Biology 2019Quote: Mouse CD3 positive T cells were isolated from spleens collected from CEA transgenic mice using negative selection kit (Stemcell Technologies, Canada) and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human CD4+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 and 15023). T cells were cryopreserved in RPMI-1640 (Corning #10-040-CV ...
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bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
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bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
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bioRxiv - Synthetic Biology 2021Quote: Primary CD4+ and CD8+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 & 15063). Blood was obtained from Blood Centers of the Pacific (San Francisco ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens or mesenteric lymph nodes (MLNs) using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). Where indicated ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Immunology 2021Quote: Primary human CD4+ T cells were isolated from PBMCs using a Human resting CD4+ T Cells Isolation Kit I according to the manufacturer instructions (STEMCELL Technologies). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... Bulk CD3+ T cells or an isolated CD3+CD8+ T cell subset were extracted from whole blood using negative selection methods (RosetteSep, StemCell Technologies) and cultured in ImmunoCult-XF T cell Expansion Medium (StemCell Technologies) ...
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bioRxiv - Immunology 2019Quote: ... cryopreserved PBMCs were thawed (as above) and CD4 T cells were isolated by negative selection using the EasySep Human CD4 Enrichment Kit (Stemcell Technologies) before DNA extraction with the QiaAMP Blood Mini Kit (Qiagen) ...