Labshake search
Citations for Stemcell Technologies :
501 - 550 of 722 citations for L Phenylalanine N T Boc 13C9 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml SCF and 10 ng/ml Flt3-L (Stem Cell Technologies). 1 x 106 transduced HSPCs were resuspended in PBS and injected into the tail vein of 6-week-old female Balb/c mice ...
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bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Murine CD4 T cells were isolated from processed lymphoid organs using EasyEights magnet (Stem Cell Technologies, Catalog no. 18103) and the whole CD4+ T cell isolation kit (Stem Cell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Immunology 2020Quote: ... and used directly for total T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Immunology 2021Quote: ... Bulk T cells were isolated from Leukopaks using EasySep magnetic selection following manufacturers’ recommended protocol (Stemcell Technologies cat 17951). Immediately following isolation ...
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bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC) as previously described (2) ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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bioRxiv - Biochemistry 2022Quote: T cells were isolated from peripheral blood mononuclear cells (PBMCs) using the RoboSep-S cell isolation platform (STEMCELL Technologies) with EasySep Human T cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Microbiology 2023Quote: Phosphorylation status of p65 and c-JUN were analyzed by flow cytometry in isolated CD4 T cells (StemCell Technologies) as previously described (44) ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Biophysics 2023Quote: Primary human CD4+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were cultured in collagen-I gels in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, Cat# 10981) with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T cells were purified to >90% from the spleens of donor strains using negative selection (StemCell Technologies) and stained with CTV or CTFR proliferation dyes (Molecular Probes ...
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells from Thy1.1+ P14 mice (PBMCs) were enriched using a CD8 MACS-negative selection kit (STEMCELL Technologies). ∼35,000 P14 CD8+ T cells were transferred intravenously into naïve Thy1.2+ C57BL/6 recipient mice ...
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bioRxiv - Immunology 2023Quote: ... naïve CD45.1/CD45.2+ P14+ CD8+ T cells were purified from spleens using the StemCell CD8+ isolation kit (STEMCELL Technologies) and 500 were adoptively transferred into CD45.1+ mice ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Genomics 2020Quote: ... CD4 and CD8 T cells were isolated from a mouse using CD4 and CD8 negative selection kits respectively (StemCell Technologies) and were labelled with the caged strand hybridized to the Ab-oligo conjugate ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies). CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies) ...
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bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
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bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads according to the manufacturer’s instructions (StemCell Technologies). The cells were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... 2D2 and OT-I cells were isolated respectively with magnetic CD4+ and CD8+ T cell selection beads (EasySep, STEMCELL Technologies).
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC, Canada). Isolated naïve CD8+ cells (1 x 106 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Immunology 2023Quote: OVA-specific CD8+ T cells were isolated from the spleens and lymph nodes of OTI/Thy1.1 mice by negative selection (Stemcell Technologies) and labeled with CFSE for proliferation tracking ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...