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Citations for Stemcell Technologies :
351 - 400 of 530 citations for Interleukin 10 Receptor Alpha IL10RA Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then spun down and resuspended in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% Fetal Bovine Serum (both from STEMCELL Technologies) and 100 µM Bromodeoxyuridine (BrdU ...
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bioRxiv - Molecular Biology 2019Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded and cultured in EB medium consisting of complete mTeSR1 supplemented with 10 µM Y27632 (StemCell Technologies), 50 ng/mL BMP-4 ...
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bioRxiv - Microbiology 2019Quote: ... Canada) M2-10B4 and Sl/Sl stromal cell monolayer [58] for 10-12 days in Myelocults (Stem Cell Technologies) containing 1 µM hydrocortisone and penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: Mammary glands from 10-16 weeks old C57BL/6J females were minced and dissociated using collagenase/hyaluronidase (STEMCELL Technologies) diluted (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... they were cultured during 24 hours in the presence of 10 μM of Rock inhibitor Y-27632 (StemCell Technologies).
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bioRxiv - Genomics 2022Quote: ... and quenched with DMEM/F-12 GlutaMAX supplemented with 10 µM of ROCK inhibitor (Y-27632, StemCell Technologies, #72304) and 0.033 mg/ml of DNase (DNase (D2) ...
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bioRxiv - Immunology 2023Quote: ... The diluted collar blood was then layered on top of 10 mL Lymphoprep™ density gradient medium (Stemcell Technologies). After centrifugation at 1200 g for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Bioengineering 2023Quote: ... 1G4 PBMCs loaded onto pMHC nanovials were sorted based on calcein AM and NGFR signal and reconstituted with 0.75 mL media and 0.25 mL of 10 mg/mL Collagenase Type II solution (STEMCELL Technologies), followed by a 2 hour incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and cultured overnight in mTeSR Plus medium supplemented with 10 µM Y-27362 ROCK inhibitor (Stem Cell Technologies, Canada). Differentiation was induced on day 0 by first washing cells with PBS when the monolayer reached approximately 80% confluence ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregates were cultured from day 8 to day 10 in A-RPMI or TeSR™-E6 (StemCell Technologies 05946) medium supplemented with 25% M.CM ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were initially plated on 10% Matrigel coated plates and expanded in PneumaCult™-Ex Plus Medium (Stemcell Technologies) + 1x PneumaCult™-Ex Plus Supplement (Stemcell Technologies ...
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bioRxiv - Biophysics 2023Quote: ... the top side was coated with 10 µg/ ml of CellAdhere™ Laminin-521 (Cat. No.77003, STEMCELL Technologies) for 3 hours at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Cell Biology 2020Quote: ... Single cell suspensions were enriched for NK lineage cells using a bivalent antibody RosetteSep (StemCell Technologies)-based method (55) ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2023Quote: ... Basophils were purified to >99% using negative selection antibodies and beads (STEMCELL Technologies, Cambridge, MA, USA).22 The pDC were prepared from the BDC suspension using BDCA-4+ selection after conducting 2 cycles of CD14+ selection to first remove monocytes.23
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bioRxiv - Cancer Biology 2019Quote: ... Prior to use for transplanting mice the cells were thawed in Iscove’s modified Dul-becco medium with 10% FBS (both STEMCELL Technologies) and 10 mg/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... Skin was disinfected with 10% iodine in phosphate saline buffer (PBS) and incubated with 2U/ml of dispase (STEMCELL technologies) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and plated in single cells at 50,000 cells/cm2 in mTeSR Plus media supplemented with 10 µM Y-27632 (STEMCELL Technologies) on tissue culture plates coated with 336.67 µg/mL Growth Factor Reduced matrigel (Corning) ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Physiology 2022Quote: ... at an optimised density of 0.8-1.3×106 cells/well in mTeSR1 supplemented with 10 µM Y-27632 (StemCell Technologies, UK). Once cells had attached (6+ hours) ...
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bioRxiv - Genomics 2019Quote: ... and quenched with complete STEMflex media containing 10-µM RHO/ROCK pathway inhibitor (Ri) Y-276932 (STEMCELL Technologies Cat#72304). Cells were subsequently spun down at 300 rcf for 5 minutes and resuspended in media supplemented with Ri ...
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bioRxiv - Genomics 2021Quote: ... For crypt isolation the intestinal tissue pieces were transferred into 10 ml of Gentle Cell Dissociation Reagent (07174, STEMCELL Technologies) and incubated for 15 mins at room temperature (RT) ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... counted and added at 1.2 million cells per microwell in AggreWell media containing 10 μM ROCK inhibitor (StemCell technologies 72302). Media was carefully changed the following day to remove ROCK inhibition ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: B cells subsets were obtained from WT or IL17 −/− naïve mice or from mice 14 d after alloantigen immunization (2 × 10 7 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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bioRxiv - Cell Biology 2024Quote: FACS-sorted GFPhi MAPs from mammary glands of PdgfrαH2B-eGFP mice were seeded onto collagen-coated wells of a 24-well plate at 20,000 cells per well and cultured in DMEM media with 10% FBS and 10ng/ml bFGF (STEMCELL Technologies). Following 5 days of culture ...
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bioRxiv - Molecular Biology 2024Quote: ... and differentiation protocol as reported.10 At day -1 iPSC were prepared in mTeSR™1 (StemCell Technologies Cambridge, MA) and plated at approximately 40 colonies per well on Matrigel pre-coated wells ...
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bioRxiv - Developmental Biology 2024Quote: ... for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies) and cultured for 7 days with daily medium exchange ...
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bioRxiv - Immunology 2019Quote: ... Antibody-bound cells were depleted with Rapidsphere streptavidin magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies).
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cell suspension-matrigel mixture was placed in a transwell and incubated in growth media with 10 μM ROCK inhibitor (Cat#72252; STEMCELL Technologies) in a 24 well plate for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... and resuspended to a final concentration of 10 000 cells/50 μL in EB media: mTeSR™ Plus medium (StemCell Technologies), 50 ng/mL BMP-4 (R&D ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at −80°C until analysis ...
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bioRxiv - Biochemistry 2019Quote: ... collected by 5 min centrifugation at 300 × g and resuspended in mTeSR1 supplemented with 10 µM Rock inhibitor Y-27632(2HCl) (72304, StemCell Technologies) for the first day.
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bioRxiv - Physiology 2020Quote: ... Single-cell suspensions were plated at a density of 1.2 × 105 cells/cm2 and cultured overnight in mTeSR medium supplemented with 10 μM Y-27632 dihydrochloride (Stem Cell Technologies). Once the monolayer reached approximately 80% confluence (usually the following day) ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Neuroscience 2021Quote: ... were isolated from E13.5 pregnant CD-1 mice and cultured on poly-D-lysine (20 μg/mL) and laminin (10 μg/mL)-coated plates in NeuroCult Basal Medium containing NeuroCult Proliferation Supplement (StemCell Technologies) and 20 ng/mL EGF ...
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bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2021Quote: ... The resulting single cell suspension was than washed with 10 mL of cold RPMI and resuspended in 1 mL of Robosep buffer (STEMCELL technologies) prior to the negative selection of B- or T-cells ...