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Citations for Stemcell Technologies :
401 - 450 of 1053 citations for IL 7 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Human CFU assays were performed as per manufacturer’s instructions (#04445, STEMCELL Technologies, Inc.). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... hPSCs (human pluripotent stem cells) were cultured with mTeSRTM Plus medium (STEMCELL Technologies) on GeltrexTM (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Mo were then isolated with EasySep Human Monocyte Enrichment Kit (Stem Cell Technologies) following the manufacturer protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the EasySep™ Human T Cell Isolation Kit (Stemcell Technologies, Cat.#17951).
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bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... using EasySepTM Human CD11b Positive Selection and Depletion Kit (StemCell Technologies, 100-0742) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Human blood products from healthy donors were purchased from StemCell Technologies (Vancouver, Canada). Peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Bioengineering 2023Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched (Human Pan-B cell Enrichment Kit [19554, StemCell Technologies]) and then stained with viability dye FVS780 (565388 ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the EasySep™ Human T Cell Isolation Kit (Stemcell Technologies, Cat.#17951), with EasySep™ Buffer (Cat.#20144 ...
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bioRxiv - Neuroscience 2024Quote: ... human recombinant shh (C24II) (cat# 78065.1) (all from STEMCELL Technologies, Tullamarine, VIC, Australia). See table S2 for antibodies used for ICC.
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bioRxiv - Genomics 2024Quote: ... 250 µL of ImmunoCult Human CD3/CD28 T Cell Activator Mix (StemCell Technology) was added to the cells and incubated at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus media was removed after 24 hours and the cells were allowed to rest for another 24 hours before being selected for 7 days with Puromycin at 8µg/ml (73342, STEMCELL Technologies, Cambridge, MA, USA). Surviving cells were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
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bioRxiv - Immunology 2021Quote: ... Poland using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... followed by magnetic separation using the EasySep™ human PE selection kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs) were routinely cultured in mTeSR1 medium (Stem Cell Technologies) on growth factor-reduced Matrigel-coated dishes (BD Biosciences ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies (Vancouver, Canada). Cells were thawed per manufacturer’s instructions and cultured as previously described.60 Briefly ...
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bioRxiv - Genomics 2020Quote: Human iPSC-derived neural progenitor cells (XCL4) were acquired from STEMCELL technologies (cat #70902) and grown in Neural Progenitor Medium 2 ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf) (StemCell Technologies, Cat 02634), 2 μg/mL Heparin (StemCell Technologies ...
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bioRxiv - Genomics 2021Quote: ... with 10 ul ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies), 50 ng/ml IL-1B (R&D Systems) ...
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bioRxiv - Bioengineering 2020Quote: ... Bulk T cells were isolated using RosetteSep Human T cell Enrichment Cocktail (StemCell Technologies). Peripheral mononuclear blood cells (PBMCs ...
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bioRxiv - Bioengineering 2021Quote: ... and 40 ng/mL animal component-free human recombinant Activin A (STEMCELL Technologies, 78132) for 12 days with media changes every other day ...
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bioRxiv - Bioengineering 2020Quote: T-cells were isolated with the EasySep Human T-cell isolation Kit (STEMCELL Technologies) from frozen human peripheral blood mononuclear cells obtained from healthy donors ...
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bioRxiv - Neuroscience 2021Quote: ... The FITC Selection Cocktail from the EasySep Human FITC Positive Selection Kit (STEMCELL Technologies) was added at a dilution of 1:10 and the cell suspension was incubated for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The colonoids were maintained in Human IntestiCult™ Organoid Growth Medium (STEMCELL Technologies Inc.).
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bioRxiv - Bioengineering 2021Quote: ... from anonymous healthy human donor blood collected by leukopheresis and purchased from STEMCELL technologies. T cells were cryopreserved in CryoStor10 (STEMCELL technologies) ...
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bioRxiv - Microbiology 2021Quote: ... or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Negative selection was performed using EasySep Human CD4+ T Cell Enrichment Kit (Stemcell Technologies) after Lymphoprep density gradient (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and activated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Stem Cell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... PBMCs and ImmunoCult Human CD3/CD28 T cell activators were obtained from Stemcell Technologies.
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bioRxiv - Immunology 2022Quote: Human embryonic stem cells (H9, WiCell, WA09) were cultured with mTeSR (STEMCELL Technologies, 85850) on Vitronectin XF (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells are plated on human vitronectin VTN XFTM (STEMCELL Technologies, Canada. Catalog#100-0763) coated 100mm cell culture dishes ...
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, 19666) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: All human iPSCs were maintained in feeder-free conditions in mTeSR1 medium (STEMCELL Technologies) on Matrigel-coated plates (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 30 ng/mL human recombinant epidermal growth factor (rhEGF) (STEMCELL Technologies 78006) and filtered through a 40 μm filter (Fisherbrand™ 22-363-547 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC (XCL-1) derived neural progenitor cells (NPCs, Stem Cell Tech cat#70901) were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560 ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: Human primary monocytes were isolated from whole blood using Sepmate tubes (StemCell Technologies, #85450) in combination with RosetteSep Human Monocyte Enrichment Cocktail (StemCell Technologies ...