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Citations for Stemcell Technologies :
351 - 400 of 569 citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Splenic B cells or T cells were Isolated with EasySepTM Mouse B Cell Isolation Kit (#19854, StemCell Technologies) or EasySepTM Mouse T Cell Isolation Kit (#19851 ...
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bioRxiv - Cancer Biology 2023Quote: ... the resulting single cells were sorted on CD45 using EasySepTM Mouse CD45 Positive Selection Kit (StemCell Technologies, 18945) and used for downstream assays ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were purified from P14 spleens using EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies). Cells were then expanded using plate-bound CD3/CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Neuroscience 2024Quote: ... mature microglial cells were isolated from astrocytes using EasySep Mouse CD11b Positive Selection Kit II (Stemcell Technologies #18970A,) and subsequently seeded into p60 plates ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were further lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... spleen-derived B-cells were selected using the EasySep™ Mouse Pan-B Cell Isolation Kit (STEMCELL Tech).
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bioRxiv - Genetics 2024Quote: ... HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
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bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured as described above (see Primary NSC cell culture) until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: NSCs were cultured as described above (see “Primary NSC cell culture”) in complete activated media until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well in Matrigel (Corning ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... total CD8+ T cells were first enriched using the EasySep™ Mouse CD8+ T cell isolation kit (Stemcell Technologies) and then CD8+CD44hiCD62Llo central memory T cells purified by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were isolated for intravenous injection using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies, Canada). 4×106 isolated T cells were injected intravenously per mouse.
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bioRxiv - Immunology 2021Quote: ... Macrophages were separated from fibroblasts with the use of the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to phycoerythrin.
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bioRxiv - Immunology 2021Quote: ... Poly I:C-activated NK cells were isolated from the spleen using EasySep Mouse NK Cell Isolation Kit (STEMCELL Technologies). Purified NK cells were co-cultured with target cells at a ratio of 5:1 ...
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bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Immunology 2020Quote: ... splenic NK cells were enriched to >80% purity using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies), and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
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bioRxiv - Developmental Biology 2019Quote: ... Neurospheres were grown on non-tissue culture treated plates with mouse NeuroCult proliferation media (Stem Cell Technologies; Seattle, WA) containing 10 ng/ml bFGF ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched from the SCS using Mouse Pan-B Cell Isolation kits (Stemcell Technologies, Vancouver, BC, Canada). Briefly ...
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Immunology 2019Quote: OT2 T cells were isolated by magnetic beads (EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit, StemCell Technologies) from a single cell suspension of splenocytes ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Cancer Biology 2021Quote: ... DF-AA27 GEMM GSCs were cultured in NeuroCult Basal Medium (Mouse and Rat) with Proliferation Supplement (StemCell Technologies 05702) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in wells of a 24-well plate containing 650 μl IntestiCult mouse organoid growth medium (StemCell Technologies cat# 06005) at 37°C/5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... and lineage negative cell fraction were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies #19856). Cell sorting of LSK or LT-HSC ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from total homogenate using the EasySep Mouse CD8+ T-cell (Stem Cell Technologies, 19853) isolation kit and EasyPlate EasySep magnets (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated by magnetic sorting (EasySep™ Mouse CD8+ T Cell Isolation Kit, StemCell Technologies), followed by tetramer E7 and TRM marker staining ...
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bioRxiv - Immunology 2023Quote: ... dendritic cells (DCs) were enriched by magnetic isolation using the EasySepTM Mouse Pan-DC Enrichment Kit (Stem Cell Technologies). DCs were plated into 96-well U-bottom plates (100 × 106 cells/well) ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...