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Citations for Stemcell Technologies :
651 - 700 of 856 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: NK cells were purified from uninfected spleens of WT and CypD-/- mice using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies) according to the supplier’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: T lymphocytes were isolated from the spleen of C57BL/6J mice using a negative selection isolation kit (EasySep™ Mouse T Cell Isolation Kit, StemCell Technologies). Cells were plated in a 48 wells plate (Corning ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2020Quote: ... from individual mice were pooled and pre-enriched for CD4 T cells using EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies). Live ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... lineage depletion was performed as follows: added 20 µl of the EasySep mouse hematopoietic progenitor cell isolation cocktail (Stem Cell Technologies 19856), incubated for 15 min ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... The cells were cultured in feeder-free conditions in 10 cm culture dishes in TeSR-E8 (Stem Cell Tech) medium on SyntheMAX (2µg/cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then spun down and resuspended in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% Fetal Bovine Serum (both from STEMCELL Technologies) and 100 µM Bromodeoxyuridine (BrdU ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded and cultured in EB medium consisting of complete mTeSR1 supplemented with 10 µM Y27632 (StemCell Technologies), 50 ng/mL BMP-4 ...
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bioRxiv - Microbiology 2019Quote: ... Canada) M2-10B4 and Sl/Sl stromal cell monolayer [58] for 10-12 days in Myelocults (Stem Cell Technologies) containing 1 µM hydrocortisone and penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: Mammary glands from 10-16 weeks old C57BL/6J females were minced and dissociated using collagenase/hyaluronidase (STEMCELL Technologies) diluted (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... they were cultured during 24 hours in the presence of 10 μM of Rock inhibitor Y-27632 (StemCell Technologies).
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bioRxiv - Genomics 2022Quote: ... and quenched with DMEM/F-12 GlutaMAX supplemented with 10 µM of ROCK inhibitor (Y-27632, StemCell Technologies, #72304) and 0.033 mg/ml of DNase (DNase (D2) ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Immunology 2023Quote: ... The diluted collar blood was then layered on top of 10 mL Lymphoprep™ density gradient medium (Stemcell Technologies). After centrifugation at 1200 g for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... 1G4 PBMCs loaded onto pMHC nanovials were sorted based on calcein AM and NGFR signal and reconstituted with 0.75 mL media and 0.25 mL of 10 mg/mL Collagenase Type II solution (STEMCELL Technologies), followed by a 2 hour incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and cultured overnight in mTeSR Plus medium supplemented with 10 µM Y-27362 ROCK inhibitor (Stem Cell Technologies, Canada). Differentiation was induced on day 0 by first washing cells with PBS when the monolayer reached approximately 80% confluence ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were initially plated on 10% Matrigel coated plates and expanded in PneumaCult™-Ex Plus Medium (Stemcell Technologies) + 1x PneumaCult™-Ex Plus Supplement (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregates were cultured from day 8 to day 10 in A-RPMI or TeSR™-E6 (StemCell Technologies 05946) medium supplemented with 25% M.CM ...
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bioRxiv - Biophysics 2023Quote: ... the top side was coated with 10 µg/ ml of CellAdhere™ Laminin-521 (Cat. No.77003, STEMCELL Technologies) for 3 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2019Quote: ... Initial HSPC isolation was performed using defined protocols of EasySep™ Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (#19756, #18000, Stemcell Technologies, CA). A secondary isolation further enriched the HSPC population by collecting the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Cancer Biology 2020Quote: ... from wild-type or Jak1 floxed C57/Bl6 mice were isolated using EasySep mouse hematopoietic progenitor cell isolation kit (Stemcell technologies, Vancouver, Canada) which remove lineage marker-expressing cells with biotinylated antibodies directed against non-hematopoietic stem cells and non-progenitor cells (CD5 ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... the cells were needle triturated and resuspended in Mouse NSC Proliferation Media (1x Anti-Anti, 10ng/mL bFGF (#78003, Stem Cell Tech, Vancouver), 20ng/mL mEGF (#78016 ...
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bioRxiv - Developmental Biology 2022Quote: ... and human naïve iPSCs (HPD06 and HPD01 were previously generated by direct reprogramming from somatic cells and described in13) were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs; DR4 ATCC) in PXGL medium36 or in RSeT medium (Stem Cell Technologies 05969). The PXGL medium was prepared as follows ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...