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Citations for Stemcell Technologies :
401 - 450 of 969 citations for IA2 Human 365a.a His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... T cells were purified using the RosetteSep Human T Cell Enrichment Kit (Stem Cell Technologies) with the Lymphoprep density gradient medium and SepMate-50 tubes ...
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bioRxiv - Immunology 2022Quote: ... Total blood DCs were enriched with EasySep human pan-DC preenrichment kit (Stemcell Technologies 19251). DC enriched fraction were stained with antibodies specific for HLA-DR APCeFluor780 ...
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bioRxiv - Immunology 2020Quote: Cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, STEMCELL Technologies). Purified NK cells were then cultured in stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...
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bioRxiv - Immunology 2021Quote: Human peripheral blood from de-identified healthy donors was purchased from STEMCELL Technologies (Vancouver, Canada), which collects and distributes de-identified human blood products with consent forms ...
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bioRxiv - Immunology 2021Quote: ... T cells were further enriched using the EasySep Human T Cell Isolation Kit (STEMCELL Technologies), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Enriched HSPC-pDCs were then primed for 1-3 days in RF10 (RPMI-1640 medium (Merck ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s protocol (EasySepTM Human Plasmacytoid DC Enrichment kit, STEMCELL Technologies Cat#19062). Obtained cells were phenotypically validated by flow cytometry.
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bioRxiv - Cancer Biology 2021Quote: ... human CD41+ cells were isolated using the CD41+ selection TAC kit (Cat# 10450, StemCell Technologies).
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bioRxiv - Immunology 2022Quote: ... and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology, #10970) with or without 2µM of NU74411 for 48 hours.
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were magnetically isolated (#100-0694 EasySep human positive selection kit II, STEMCELL Technologies) and the resulting cell suspension was passed through a 70 μm cell strainer.
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bioRxiv - Immunology 2022Quote: ... NK cells were removed from cultures (EasySep Human CD56 Positive Selection Kit II, StemCell Technologies). Remaining CD4 T cells were analyzed by flow cytometry to confirm removal of NK cells by staining for CD56 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) by immunomagnetic negative selection according to manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were isolated using EasySepTM Direct Human T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 5iLA naive human PSCs were dissociated into single cells by incubation with Accumax (Stemcell technologies) for 3 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... plated in matrigel and cultured with 500 μl/well human IntestiCult™ OGM (Stemcell Technologies). Medium was changed every 2-3 days and organoids were passaged once a week ...
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bioRxiv - Synthetic Biology 2022Quote: ... T cells were isolated using the RosetteSep Human T Cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... with an additional granulocyte depletion step using the RosetteSep Human Granulocyte Depletion Cocktail (STEMCELL Technologies) as directed by the manufacturer ...
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Autologous humanized PDX modeling for immuno-oncology recapitulates the human tumor microenvironmentbioRxiv - Cancer Biology 2022Quote: ... CD34+ cells were positively selected using the EasySep Human CD34 Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Human blood-derived mast cells were plated between 40,000–50,000/well in StemSpan (StemCell Technologies) serum-free medium supplemented with Stem Cell Factor (Peprotech ...
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bioRxiv - Genetics 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained in mTeSR+ medium (Stem Cell Technologies #05825) on tissue culture-treated plates coated with Matrigel (Corning #354277) ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived hematopoietic progenitors (HPC) were generated using the STEMdiff Hematopoietic Kit (STEMCELL Technologies) according to the manufacturer instructions based on a protocol by Abud et al. ...
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bioRxiv - Immunology 2023Quote: ... we used mouse-specific and human-specific T cell negative isolation MACS (STEMCELL Technologies, Canada). We plated 0.5–1 × 106 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Immunology 2023Quote: ... CD14+ Mo were FACS sorted from frozen human bone marrow mononuclear cells (Stem Cell Technologies) on a BD FACS Melody Cell Sorted (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PBMCs were activated with ImmunoCult human CD3/CD28 T cell activator (STEMCELL Technologies; Cat. # 10971) and 50 U/mL IL-2 (BD-Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: T cells were enriched using the Easy Sep Human T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Stimulation was done using ImmunoCult human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies) in non-polarizing conditions using anti-IL-4 (5 μg/ml) ...
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bioRxiv - Genomics 2023Quote: ... WTC11 human iPSCs (RRID:CVCL_Y803) were cultured in mTeSR plus medium (Stemcell technologies, Catalog # 100-0276) and passaged using ReLeSR (Stemcell technologies ...
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bioRxiv - Immunology 2023Quote: ... and CD34+ HSPCs were enriched using the EasySep Human CD34+ Positive Selection kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... medium was replaced by 250µL of Intesticult ODM human basal medium (StemCell technologies, 100-0214), allowing generation of intestinal organoid cultures with physiological representations of the stem cell and differentiated population ...
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bioRxiv - Developmental Biology 2023Quote: Shef6 human ESCs were cultured on Matrigel-coated plates in mTESR medium (05825, STEMCELL Technologies) at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... EasySep™ human CD34 positive selection kit II was procured from Stem cell technologies (17856). Ficoll® Paque PLUS density gradient media was purchased from Sigma Aldrich (GE17-1440-03).
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bioRxiv - Neuroscience 2023Quote: ... Human DS and control iPSCs were cultured and maintained in mTeSR1 medium (Stem Cell Technologies) in tissue culture dishes coated with hESC-qualified Matrigel (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... H1 human embryonic stem-cell (H1-hESC) line was cultured in mTeSR1 medium (STEMCELL Technologies) under 5% CO2 at 37℃ and was sub-cultured when reaching 80% confluence ...
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bioRxiv - Bioengineering 2023Quote: ... Human induced pluripotent stem cell (hPSC) line PGP1 was cultured with mTeSR1 (STEMCELL Technologies, #85850) in tissue culture 6-well plates coated with reduced growth factor Geltrex (Gibco ...
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bioRxiv - Microbiology 2023Quote: A three-stage protocol was used to differentiate human bone marrow CD34+ cells (STEMCELL Technologies) down the erythroid lineage as previously described.37 Briefly ...
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bioRxiv - Immunology 2023Quote: ... PBMCs from human blood samples were isolated on a density gradient medium (Lymphoprep, STEMCELL Technologies). Cells were incubated 4°C for 10 min with anti-mouse or anti-human CD16/CD32 (BD Pharmingen ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with 25μL/mL ImmunoCult Human αCD2/αCD3/αCD28 T cell Activator (STEMCELL Technologies) or 10μL/mL T-cell TransAct αCD3/αCD28 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies, 17955) and activated by 100 Gy irradiated K562 Clone 9 feeder cells ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were enriched from PBMC by negative selection using EasySepTM human monocyte kit (STEMCELL Technologies) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: G-CSF Mobilized Human Peripheral Blood CD34+ Cells were purchased from StemCell Technologies (Catalog: 70060.1). Cells were thawed by resuspending in 10 mL of RPMI without FBS ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated by EasySep™ Human T Cell Isolation Kits (STEMCELL Technologies, 17951). Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2021Quote: Human ESCs were differentiated into neural progenitor cells (NPCs) using the STEMdiff neural system (STEMCELL Technologies). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc.). The resulting CD34+ HSPCs were cultured in HSPC media ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line (WA01, WiCell) was maintained with mTeSR plus medium (Stemcell Technologies) on 6 well plates coated with Matrigel (Corning) ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpan™ medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2019Quote: ... Pure cell populations were isolated using EasySep Human negative selection kits (STEM CELL technologies, >95% purity). In addition ...