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Citations for Stemcell Technologies :
351 - 400 of 1831 citations for Human Large Proline Rich Protein BAG6 BAG6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Preliminary experiments were also performed after isolation of monocytes using the EasySep human monocyte enrichment kit with CD16 depletion (StemCell Technologies) and the EasySep human monocyte isolation kit (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
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bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
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bioRxiv - Immunology 2020Quote: ... Total mDC and pDC were negatively enriched from PBMCs using the EasySep™ Human Pan-DC Pre-Enrichment Kit (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
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bioRxiv - Genomics 2021Quote: Human ES cells were differentiated using the STEMdiff Definitive Endoderm Kit (TeSR-E8 Optimized) from STEMCELL Technologies (Ramme et al. 2019). Rat ES cells were differentiated following an optimized mouse protocols (Kim et al ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
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bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Microbiology 2022Quote: ... primary CD4+ T-cells were negatively enriched using EasySep Human CD4+ T-cell isolation kit (Catalog no. 17952; Stem Cell Technologies) and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2 ...
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bioRxiv - Molecular Biology 2022Quote: ... density gradient centrifugation and total CD4+ T cells were enriched by negative selection with the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies) to an average purity of 90% ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4 T cells were isolated from PBMCs by negative selection using EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, #19052). All participants signed informed consent forms approved by the Ethics Committee of the Ghent University Hospital (Belgium ...
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bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from thawed PBMCs by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM EDTA buffer and monocytes were then isolated using the magnetic EasySep Human CD14+ Selection kit (STEMCELL Technologies, Vancouver, BC, Canada) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were obtained at >95% purity from pooled collections using a two-step Rosette-Sep/EasySep human CD34-positive selection kit (STEMCELL Technologies) according to the manufacturer’s protocols and/or FACS sorting ...
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bioRxiv - Immunology 2024Quote: ... T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada). Second- generation 4-1BB- costimulated TSHR CAR constructs were synthesized de novo (IDT ...
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bioRxiv - Immunology 2023Quote: ILC were isolated from PBMCs by negative selection using the EasySep™ Human Pan-ILC Enrichment Kit according to manufacturer’s instructions (Stemcell Technologies). ILC were sorted using FACSARIA™IIu (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were isolated from PBMCs using an EasySep Human CD4+ T Cell Isolation Kit (17952, StemCell Technologies, Canada) as per manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... the cell suspension resulting from the dissociation was enriched in CD45+ cells with an EasySep Release Human CD45 Positive Selection Kit (Stemcell Technologies) following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Bioengineering 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights™ EasySep™ Magnets (StemCell Technologies) according to the manufacturer protocol.
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bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
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bioRxiv - Biophysics 2024Quote: NK cells were obtained from anonymous buffy coats (Karolinska Hospital, Huddinge, Sweden) by negative selection using EasySep Direct Human NK cell isolation kit (Stemcell Technology) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
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bioRxiv - Immunology 2021Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15 ...
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bioRxiv - Immunology 2021Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
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bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
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bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.
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bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2019Quote: Neutrophils were isolated from peripheral venous blood using a Percol density gradient as described previously (17) and purified using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies) as per the manufacturer’s instructions (18) ...
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bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...