Labshake search
Citations for Stemcell Technologies :
451 - 500 of 1849 citations for Human Gastric Intrinsic Factor GIF CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection) following the manufacturer’s instructions (EasySepTM Human cord blood CD34+ positive selection kit II, STEMCELL Technologies Cat#17896). CD34+ HSPCs were either freshly used or cryo-preserved until future use ...
-
bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
-
bioRxiv - Microbiology 2022Quote: Memory CD4+ T cells were enriched by negative selection using the EasySep Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies #19157) following the recommended protocol ...
-
bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were subsequently isolated from the PBMC population by immunomagnetic negative selective with the EasySep Human T Cell Isolation kit (StemCell Technologies, Canada). Isolated T cells with >95% viability and >95% purity were either cryopreserved or directly cultured for subsequent experiments ...
-
bioRxiv - Genetics 2023Quote: Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation and classical monocytes isolated using EasySep™ Human Monocyte Isolation Kit from Stemcell Technologies according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... CD14+CD16-monocytes were isolated from peripheral blood mononuclear cells using immunomagnetic negative selection (EasySep Human Monocyte Isolation Kit, Stem Cell Technologies).
-
bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
-
bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
-
bioRxiv - Immunology 2024Quote: For further examination of neutrophil granulocytes we isolated granulocytes with the EasySep® Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Cat# 19666), following the instructions provided by the manufacturer ...
-
bioRxiv - Synthetic Biology 2020Quote: Human natural killer cells (Stemcell Technologies, Canada) were incubated with 5×105 skin fibroblasts for 60 min at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... and human (MethoCult™ H4434, Stemcell Technologies) differentiating PSC ...
-
bioRxiv - Immunology 2022Quote: Cryopreserved primary human CD14+ monocytes (Stemcell Technologies) isolated from peripheral blood were plated at 300,000 cells/well in flat bottom ...
-
bioRxiv - Cell Biology 2023Quote: Human PBMCs were purchased from STEMCELL Technologies and Precision for Medicine ...
-
bioRxiv - Cancer Biology 2023Quote: ... human CD8+ T cells (StemCell Technologies, 70027), CD4+ T cells ...
-
bioRxiv - Cell Biology 2023Quote: ... human LDL (50ng/ml) (Stem Cell Technologies) in addition to the following cytokines ...
-
bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human FGF2 (Stemcell Technologies) + 500 nM A-8301 (Selleckchem ...
-
bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 10 vol% conditioned medium containing Rspondin1 (produced from HA-R-Spondin1-Fc HEK293T cell line ...
-
bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 3 µM CHIR9902 (Stemcell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human FGF2 (Stemcell Technologies) + 500 nM A-83-01 + 1:500 Primocin + 10 µM Y-27632 (only for the first two days) ...
-
bioRxiv - Immunology 2024Quote: ... 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... frozen human PBMCs (STEMCELL Technologies, Vancouver, Canada) were thawed and seeded in a 75 cm2 flask at a concentration of 5 × 106 cells/mL in RPMI 1640 media (Corning Inc. ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were cultured in NeuroCult™ Proliferation Medium containing 20 ng/ml epidermal growth factor (EGF) (Stemcell Technologies). For differentiation assays ...
-
bioRxiv - Biophysics 2023Quote: Primary human CD4+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
-
bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
-
bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
-
bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
-
bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
-
bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
-
bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
-
bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
-
bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
-
bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
-
bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
-
bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
-
bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
-
bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...