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Citations for Stemcell Technologies :
51 - 100 of 1351 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were kept in a 37°C tissue culture incubator with 5% CO2 and lines were maintained in mTeSR Plus cultured media (Stemcell Technologies Cat# 100-1130). Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL ...
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bioRxiv - Immunology 2019Quote: ... overnight in Clonacell-HY Medium C (StemCell Technologies, Vancouver, BC, Canada), before centrifugation and resuspension in Clonacell-HY Medium E (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 75 μL of 37°C pre-warmed mTesR1 medium (Stemcell technologies) supplemented 1:10 with CloneR (Stemcell technologies ...
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bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/mL recombinant human IL-15 (StemCell Technologies, Cat # 78031). Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: ... cells were centrifuged (350 x g for 5 minutes) and resuspended in 5 mL of prewarmed 0.25% trypsin/EDTA (#07901, Stemcell Technologies) at 37°C for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
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bioRxiv - Neuroscience 2021Quote: ... at 37 °C for 1 min and seeded on AggreWell 800 (#34815; StemCell Technologies) in Essential 8 medium with ROCK inhibitor Y-27632 (10 μM ...
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bioRxiv - Cancer Biology 2019Quote: Control and c-met-mutated iPSC were maintained on Geltrex (Stem Cell Technologies, Inc) coated flat culture dish in E8 media (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2021Quote: Colony-forming activity (CFU-C) assays were performed using MethoCult M3434 (Stem Cell Technologies). A total of 2×104 BM mononuclear cells (MNCs ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 200 μL of pre-warmed (37°C) Intesticult Media (Stem Cell Technologies, Cat#06005) containing Primocin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then triturated in prewarmed (37 °C) trypsin-EDTA (0.25%; STEMCELL Technologies, 07901) with a 1 mL pipette for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... coated plates and dissociated into single cells using 37°C Accutase (Stem Cell Technologies) for ∼5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and maintained in culture medium containing mTeSR plus basal medium and 5 x supplement (Stem Cell Technologies). Thiazovivin (2 μM ...
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bioRxiv - Immunology 2019Quote: ... Human Cord Blood (CB) purified CD34+ cells from 5 different donors were obtained from STEMCELL Technologies and resuspended in StemSpan Expansion Media – SFEM II (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Cancer Biology 2020Quote: Primary FL cell-suspensions were defrosted at 37°C and layered onto 3ml of lymphoprep (STEMcell Technologies). Lymphocytes were isolated by centrifugation at 1150g for 12 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were harvested and frozen at −150°C in CryoStor® CS10 cell freezing medium (StemCell Technologies). Cell count and viability were evaluated using NC-200 NucleoCounter (Chemometec) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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bioRxiv - Bioengineering 2021Quote: ... coiled-coil containing protein kinase (ROCK) inhibitor (5 μM, StemCell Technologies, Vancouver, BC, Canada). Until 95% confluency was reached ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated 30 min at 37 °C according to the manufacturer’s instruction in ALDEFLUOR kit (01700, StemCell Technologies). For the glucose uptake analysis ...
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bioRxiv - Cancer Biology 2019Quote: Control or c-met-mutated iPSC-aggregates (iPSC-A) were plated on a Geltrex (Stem Cell Technologies, Inc.) in 24 or 96-well plates or 8-well culture chambers ...
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bioRxiv - Immunology 2021Quote: ... Cells were lifted from the plate by 15 minute incubation at 37°C with Accutase™ (StemCell Technologies), then washed with PBS + 2% FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... stem cell media is replaced with ameloblast base media consisted of either EpiCult-C media (StemCell Technologies, #05630) or RPMI 1640 Medium (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... and frozen at −80°C for qRT-PCR or Neurosphere Basal Medium supplemented with Proliferation Supplement (STEMCELL Technologies) as described below ...
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bioRxiv - Cell Biology 2023Quote: Colony assays were performed according to manufacturer’s instructions with MegaCultTM-C or MethocultTM H4435 Enriched media (StemCell Technologies) using fresh or cryopreserved HPCs ...
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bioRxiv - Developmental Biology 2023Quote: ... MEF feeders were inactivated at passage 4 with chemical treatment of 1 μg/ml mitomycin-c (STEMCELL Technologies) overnight ...
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bioRxiv - Immunology 2024Quote: ... cells were rapidly thawed at 37 °C in a water bath and added dropwise into IMDM (STEMCELL Technologies) containing 10% FBS and 10 μg/mL DNase (MilliporeSigma) ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Neuroscience 2022Quote: ... LMMP tissue was digested for 60 minutes at 37° C in dispase (250 μg/ml; STEMCELL Technologies, Vancouver, BC) and g/ml ...
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bioRxiv - Microbiology 2023Quote: Phosphorylation status of p65 and c-JUN were analyzed by flow cytometry in isolated CD4 T cells (StemCell Technologies) as previously described (44) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Immunology 2021Quote: ... and plated on Matrigel-coated plates at a density of 5 x 105 cells per cm2 in mTESR1 medium (StemCell Technologies) supplemented with 10 ng/mL of the RHO/ROCK pathway inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... centrifuged at 200 x g for 5 min prior to be resuspended in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and seeded onto growth-factor-reduced Matrigel™-coated plates (8.6µg/cm2 ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Biochemistry 2023Quote: Cells were counted and adjusted to a concentration of 5 x 105 cells/mL with ALDEFLUOR assay (01700; Stem Cell Technologies) buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... c-Kit+) from neonatal wild-type mice were FACS sorted and cultured in MethoCultTm medium (GF M3434, Stem cell technologies) in 35mm dishes ...
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bioRxiv - Developmental Biology 2022Quote: Day 15 H9 hESC-derived enteric crestospheres were dissociated into single cells (accutase, Stemcell Technologies, 07920, 30 min, 37 °C), resuspended in ENC medium and were transferred into 384-well plates ...