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301 - 350 of 1098 citations for Goat Anti Human IgG Fc FITC since 2019

• Reprogramming human B cells with custom heavy chain antibodies

  Geoffrey L. Rogers, et al., bioRxiv - Immunology 2023

  Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies and thawed per manufacturer’s instructions ...
• Multiplexed single-cell lineage tracing of mitotic kinesin inhibitor resistance in glioblastoma

  Yim Ling Cheng, et al., bioRxiv - Cancer Biology 2023

  Quote: ... were cultured with NeuroCultTM NS-A Proliferation Kit Human from STEMCELL Technologies. HEK293T were cultured with DMEM containing 10% FBS and 2 mM L-glutamine.
• Facile generation of biepitopic antibodies with intrinsic agonism for activating receptors in the tumor necrosis factor superfamily

  Harkamal S. Jhajj, et al., bioRxiv - Bioengineering 2023

  Quote: ... and ImmunoCult Human CD3/CD28 T Cell Activator (Stem Cell Technologies, 10971) was added at 5 μL/mL to a total volume to 200 μL ...
• Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy

  Min Guo, et al., bioRxiv - Bioengineering 2023

  Quote: ... with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies, Cat# 78036.3). To prepare 3 mg/ml collagen-I gel ...
• Matrix-rigidity cooperates with biochemical cues in M2 macrophage activation through increased nuclear deformation and chromatin accessibility

  Seung Jae Shin, et al., bioRxiv - Bioengineering 2024

  Quote: ... and ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL TECHNOLOGIES, #10971) for a duration of 3 days ...
• A Synthetic Cytotoxic T cell Platform for Rapidly Prototyping TCR Function

  Govinda Sharma, et al., bioRxiv - Immunology 2023

  Quote: Leukapheresis product (leukopaks) from healthy human donors were obtained from Stemcell Technologies shipped on wet ice ...
• Single-Stranded DNA with Internal Base Modifications Mediates Highly Efficient Gene Insertion in Primary Cells

  Karen L. Kanke, et al., bioRxiv - Molecular Biology 2024

  Quote: Human ABCs were cultured in PneumaCult Ex Plus medium (StemCell Technologies #05040) with addition of ROCK inhibitor 10 µM (Y-27632 ...
• A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer

  Volga M Saini, et al., bioRxiv - Cancer Biology 2024

  Quote: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001 ...
• Streptavidin-drug conjugates streamline optimization of antibody-based conditioning for hematopoietic stem cell transplantation

  Aditya R. Yelamali, et al., bioRxiv - Immunology 2024

  Quote: ... the EasySep Human Cord Blood CD34 Positive Selection Kit II (STEMCELL Technologies) was used ...
• Radical fringe facilitates NOTCH1 and JAG1 cis interactions to sustain Hematopoietic stem cell fate

  Roshana Thambyrajah, et al., bioRxiv - Developmental Biology 2023

  Quote: AGMs of E10.5 embryos were dissected in PBS with 7% fetal calf serum (FCS) and penicillin/streptomycin (100 U/mL) and cultured as explants for 2 days in medium consisting of Stemspan (Stem Cell Technologies, cat # 09600), 20% fetal calf serum ...
• Hitting more birds with one stone: CD70 as an actionable immunotherapeutic target in recurrent glioblastoma

  M Seyfrid, et al., bioRxiv - Cancer Biology 2021

  Quote: ... supplemented with human recombinant epidermal growth factor (20ng/mL: STEMCELL Technologies, Cat#78006), basic fibroblast growth factor (20ng/mL ...
• Single-cell landscapes of primary glioblastomas and matched organoids and cell lines reveal variable retention of inter- and intra-tumor heterogeneity

  VG LeBlanc, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Tissues were washed once in NeuroCult NS-A Basal Medium (human; STEMCELL Technologies), and PDOs were washed in the same media at room temperature for 15 min with rocking ...
• Lack of blocking activity in anti-CTLA-4 antibodies reduces toxicity, but not anti-tumor efficacy

  Erica L. Stone, et al., bioRxiv - Immunology 2021

  Quote: Donor-matched human Treg and Tconv cells (Stemcell Technologies, 70096, Vancouver, BC, Canada) were thawed and stained with CellTrace Violet (C34571 ...
• Understanding immune-driven brain aging by human brain organoid microphysiological analysis platform

  Sunghwa Song, et al., bioRxiv - Bioengineering 2022

  Quote: Human embryonic stem cells were dissociated into single cells using ReLeSR (Stemcell Technologies) treatment ...
• Identification of CD317-Positive Pro-inflammatory Immune Stromal Cells in Human Mesenchymal Stromal Cell Preparations

  Alasdair G Kay, et al., bioRxiv - Cell Biology 2022

  Quote: ... cryopreserved CD4+ human cord blood T cells were purchased from Stem Cell Technologies. T cells were isolated from mixed T and B cell cultures using nylon wool separation96 ...
• Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages

  Glenn Oliveira, et al., bioRxiv - Evolutionary Biology 2022

  Quote: ... NPC human primary cells and supporting culture ingredients were purchased from STEMCELL technologies and the manufacturer’s instructions were followed.
• Targeted brain tumor radiotherapy using an Auger emitter

  Giacomo Pirovano, et al., bioRxiv - Cancer Biology 2019

  Quote: ... 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF) (StemCell Technologies, Cat 02633), 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf ...
• Glucuronoxylomannan in the Cryptococcus species capsule as a target for CAR⁺ T-cell therapy

  Thiago Aparecido da Silva, et al., bioRxiv - Immunology 2019

  Quote: ... An ImmunoCult human CD3/CD28 T-cell activator (STEMCELL Technologies; cat. no. 10971) was used to activate positive control T cells according to the manufacturer’s instructions ...
• Megakaryocytes Display Innate Immune Cell Functions and Respond during Sepsis

  Galit H. Frydman, et al., bioRxiv - Immunology 2019

  Quote: ... and then purified with EasySep Human Neutrophil Kit (STEMCELL Technologies Inc. Vancouver, Canada), following manufacturers protocol ...
• A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells

  Enas Abu-Shah, et al., bioRxiv - Immunology 2019

  Quote: ... Resting human T-cell subsets were isolated using negative selection kits (Stemcell Technologies), total CD4 or CD8 T-cells were enriched using RosetteSep kits from Stemcell Technologies ...
• Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies, 17664) with EasySep™ Magnet (STEMCELL Technologies ...
• Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1) in RPMI 1640 for 2 days ...
• Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 10 ng/mL human recombinant Epidermal Growth Factor (EGF, STEMCELL Technologies, 78006.1), and passaged using TrypLE™ Express Enzyme.
• A Novel Organoid Model of In Vitro Spermatogenesis Using Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 10 ng/mL human recombinant Epidermal Growth Factor (EGF, STEMCELL Technologies, 78006.1), and passaged using TrypLE™ Express Enzyme.
• A Novel Organoid Model of In Vitro Spermatogenesis Using Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 100ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1) for 2 days ...
• A Xeno-free Media for the In Vitro Expansion of Human Spermatogonial Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
• Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

  Alejandro Méndez-Mancilla, et al., bioRxiv - Bioengineering 2021

  Quote: ... and activated with the ImmunoCult Human CD3/CD28 T Cell Activator (StemCell Technologies). T cells were cultured at 37°C ...
• Human cerebral tissues created via active cellular reaggregation produce functionally interconnected 3D neuronal network to mimic pathological circuit disturbance

  Aref Saberi, et al., bioRxiv - Bioengineering 2020

  Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in mTeSR medium (STEMCELL Technologies). The cell culture flasks were coated with Matrigel (hECS-qualified matrix ...
• A ‘one-two punch’ therapy strategy to target chemoresistance in estrogen receptor positive breast cancer

  Feng Chi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies) according to manufacturer protocols ...
• DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells

  Yale S. Michaels, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 1.25% ImmunoCult Human CD3/CD28/CD2 T cell activator (STEMCELL Technologies, 10970). After 48 hours ...
• Delivery of CAR-T Cells in a Transient Injectable Stimulatory Hydrogel Niche Improves Treatment of Solid Tumors

  Abigail K. Grosskopf, et al., bioRxiv - Bioengineering 2021

  Quote: ... Negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes was performed to purify primary human T cells ...
• Heparin protects human neural progenitor cells from Zika Virus-induced cell death and preserves their differentiation into mature neural-glia cells

  Isabel Pagani, et al., bioRxiv - Microbiology 2021

  Quote: ... Cells were maintained in NeuroCult™ NS-A Differentiation Medium Human (STEMCELL Technologies) for up to 14 days.
• tiRNA signaling via stress-regulated vesicle transfer in the hematopoietic niche

  Youmna S. Kfoury, et al., bioRxiv - Cell Biology 2021

  Quote: ... Cells were isolated using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies).
• The lysine-rich intracellular loop and cell type-specific co-factors are required for IFITM3 antiviral immunity in hematopoietic stem cells

  G. Unali, et al., bioRxiv - Microbiology 2021

  Quote: Human CB-derived HSPC were cultured in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin (100 IU/ml) ...
• Immortalization and Functional Screening of Natively Paired Human T Cell Receptor Repertoires

  Ahmed S. Fahad, et al., bioRxiv - Immunology 2021

  Quote: ... and 25 μL/mL ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies). Cells were expanded in RPMI 1640 medium (Thermo Fisher Scientific ...
• Immortalization and Functional Screening of Natively Paired Human T Cell Receptor Repertoires

  Ahmed S. Fahad, et al., bioRxiv - Immunology 2021

  Quote: ... and 25 μL/mL ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies).
• Enhancer plasticity sustains oncogenic transformation and progression of B-Cell Acute Lymphoblastic leukemia

  G Corleone, et al., bioRxiv - Cancer Biology 2022

  Quote: ... the whole BM samples were incubated with Human B RosetteSep (Stemcell Technologies, CAN) following the manufacturer ‘instructions.
• Human URAT1/SLC22A12 gene promoter is regulated by 27-hydroxycholesterol through estrogen response elements

  Masaya Matsubayashi, et al., bioRxiv - Biochemistry 2019

  Quote: ... human iPS cells were treated with 8 μM CHIR99021 in APEL2 (STEMCELL Technologies) supplemented with 1% Protein Free Hybridoma Medium II (PFHM II ...
• Robust generation of erythroid and multilineage hematopoietic progenitors from human iPSCs using a scalable monolayer culture system

  Juan Pablo Ruiz, et al., bioRxiv - Developmental Biology 2019

  Quote: Human CFU assays were performed as per manufacturer’s instructions (04445, STEMCELL Technologies, Inc.). Briefly ...
• HBEGF+ macrophages identified in rheumatoid arthritis promote joint tissue invasiveness and are reshaped differentially by medications

  David Kuo, et al., bioRxiv - Immunology 2019

  Quote: ... Neutrophils were isolated with the EasySep Direct Human Neutrophil Isolation Kit (Stemcell Technologies) with “The Big Easy” EasySep Magnet ...
• GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton

  Christina Wolf, et al., bioRxiv - Neuroscience 2021

  Quote: ... Human iPS cells were detached from matrigel using ReLeSR™ (Stemcell Tech., 05872), resuspended in medium consisting of Dulbecco’s modified Eagle’s medium/ F12 supplemented with 10% knockout serum (Invitrogen ...
• Immune modulatory effects of probiotic Streptococcus thermophilus on human monocytes

  Narges Dargahi, et al., bioRxiv - Immunology 2020

  Quote: ... Monocytes were isolated using the EasySep Human Mono Isolation Kit (STEMCELL technology, Canada) [28] ...
• Molecularly distinct memory CD4+ T cells are induced by SARS-CoV-2 infection and mRNA vaccination

  Sophie L. Gray-Gaillard, et al., bioRxiv - Immunology 2022

  Quote: ... Stained cells were passed through EasySep Human PE Positive Selection Kit (STEMCELL Technologies) to enrich stimulated cells ...
• Mechanistic model-driven exometabolomic characterisation of human dopaminergic neuronal metabolism

  German Preciat, et al., bioRxiv - Systems Biology 2022

  Quote: ... The human neuroepithelial stem cells were cultivated in mTESR1 medium (StemCell technologies, #05850) on 6-well dishes coated with Matrigel (Corning ...
• Modulation of WNT, Activin/Nodal and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation During Human iPSC Differentiation

  Yongqin Li, et al., bioRxiv - Cell Biology 2023

  Quote: ... Human CFU assays were performed as per manufacturer’s instructions (#04445, STEMCELL Technologies, Inc.). Briefly ...
• Patterning and folding of intestinal villi by active mesenchymal dewetting

  Tyler R. Huycke, et al., bioRxiv - Developmental Biology 2023

  Quote: ... hPSCs (human pluripotent stem cells) were cultured with mTeSRTM Plus medium (STEMCELL Technologies) on GeltrexTM (Gibco ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... Mo were then isolated with EasySep Human Monocyte Enrichment Kit (Stem Cell Technologies) following the manufacturer protocol ...
• Engineered yeast cells simulating CD19+ cancers to control CAR T cell activation

  Marcus Deichmann, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... using the EasySep™ Human T Cell Isolation Kit (Stemcell Technologies, Cat.#17951).
• In Need of Age-Appropriate Cardiac Models: Impact of Cell Age on Extracellular Matrix Therapy Outcomes

  S. Gulberk Ozcebe, Pinar Zorlutuna, bioRxiv - Bioengineering 2023

  Quote: ... Human iPS cells were cultured routinely in mTeSR-1 media (StemCell Technologies, #05825) on 1% Geltrex-coated plates (Invitrogen ...
• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... using EasySepTM Human CD11b Positive Selection and Depletion Kit (StemCell Technologies, 100-0742) according to the manufacturer’s instructions ...