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Citations for Stemcell Technologies :
101 - 150 of 207 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Immunology 2022Quote: Bone-marrow eosinophils were isolated using EasySep™ Mouse Biotin Positive Selection Kit II (Stem Cell Technologies) according to manufacturer instructions ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HSPC: StemSpan™ SFEM II supplemented with StemSpan™ CD34+ Expansion Supplement (10X) (StemCell Technologies, Catalog # 09720). IPSC ...
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged and resuspended in mTeSR1/mTeSR plus 50:50 mix containing 10 μM Y-27632 (STEMCELL Technologies). hPSC were plated as single cells at 20,000 cells cm−2 ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies, Cat 17856) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ cells enriched from CMML patients or healthy donors were cultured in StemSpan SFEM II medium (STEMCELL Technologies) supplemented with 10% fetal bovine serum (FBS) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and cultured in StemSpan SFEM II medium supplemented with StemSpan™ CD34+ Expansion Supplement and UM729 (StemCell Technologies). HSPCs were ready for electroporation after 4-5 days in culture ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ cells were isolated from the purified PBMNCs by EasySep Human CD34 positive selection kit II (STEMCELL Technologies) and expanded in HSC expansion media as described earlier (Genovese et al ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by CD34 magnetic bead-based enrichment using the EasySep human CD34 positive selection kit II (StemCell technologies). We found this enrichment strategy to be simple and reproducible and chose it for several reasons ...
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bioRxiv - Genetics 2023Quote: ... 1 million cells were cultured for 8 days in StemSpanTM SFEM II medium (Stem Cell Technologies Catalog #09605) supplemented with 1X StemSpanTM Erythroid Expansion Supplement (Stem Cell Technologies Catalog #02692).
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the HSCs were first cultured in StemSpan™ Serum-Free Expansion Medium (SFEM) II (09655, STEMCELL Technologies, Canada) supplemented with 2 μg/mL puromycin (GC16384 ...
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bioRxiv - Neuroscience 2024Quote: ... mature microglial cells were isolated from astrocytes using EasySep Mouse CD11b Positive Selection Kit II (Stemcell Technologies #18970A,) and subsequently seeded into p60 plates ...
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bioRxiv - Cell Biology 2024Quote: ... Human CD34+ cells were magnetically enriched with the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies) from either normal BM or CB samples ...
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... For this we used a 50/50 mix between standard KOSR medium and mTESR medium (Stem Cell Technologies) and the addition of 20µM Synthechol (Sigma) ...
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bioRxiv - Genomics 2019Quote: ... CD34+ HSPCs were incubated in StemSpan SFEM II medium with StemSpan Myeloid Expansion Supplement (Stem cell technologies, Vancouver, Canada) for 10 days ...
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bioRxiv - Immunology 2020Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II for future studies (Stemcell Technologies, #17877). The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit ...
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bioRxiv - Cell Biology 2020Quote: Cord blood (CB) CD34+ cells were purified with Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, 17896) and cultured in SFEM II medium (StemCell Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Developmental Biology 2022Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemicals were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: 5,000 HSPCs were sorted directly into TC-treated 96-well plates (Falcon) containing StemSpan™ SFEM II (Stemcell Technologies) with Pen-Strep (Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 1G4 PBMCs loaded onto pMHC nanovials were sorted based on calcein AM and NGFR signal and reconstituted with 0.75 mL media and 0.25 mL of 10 mg/mL Collagenase Type II solution (STEMCELL Technologies), followed by a 2 hour incubation at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2) at a cell concentration of 5 × 105/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) supplemented with 100 ng/mL of human recombinant SCF ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were plated immediately into pre-warmed antibiotic-free HSPC media (StemSpan SFEM II; Stemcell Technologies; cat. no., 09605_C) supplemented with SCF (100 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium refreshment of the 293FT cells was done using the StemSpan SFEM II medium (Stemcell Technologies, cat# 9655). 3-4 days after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 80 µL of StemSpan™ SFEM II supplemented with StemSpan™ CD34+ Expansion Supplement (10X) (StemCell Technologies, Catalog #09720) was added to each well ...
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bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
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bioRxiv - Developmental Biology 2021Quote: ... each containing 80µL protease mix (4.5µL/mL Accutase; Stem Cell Technologies, 07920; 4.5 µL/mL Accumax; Stem Cell Technologies, 07921 ...
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bioRxiv - Genetics 2019Quote: ... Live GFP-high cells were sorted on Aria II at low flow rates and reaggregated in Aggrewell-400 (StemCell Technologies) at 1,000 cells per cluster in CMRL containing 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed twice with PBS 0.5% BSA and positively-selected by magnetic separation using PE Positive Selection Kit II (StemCell Technologies) according to manufacturer’s specifications.
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were isolated from umbilical cord blood using the EasySep™ Human CD34 Positive Selection Kit II (Stemcell Technologies). Cytapheresis of CML patients were kindly provided by Pr ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues at 19 w and 20 w were subjected to EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies, 18259) to remove CD45+ immune cells ...
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bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... the tetramer-bound fraction was enriched via an adapted protocol of the EasySep PE Selection Kit II (StemCell Technologies, Inc.) developed by Marc Jenkins’ lab ...
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼2,000 CD34+Lin-CD38-CD90loCD45RA-EPCR+ cells were cultured on tissue culture plates containing StemSpan II media (Stem Cell Technologies) supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were isolated using a EasySep Human CD34 Positive Selection Kit II according to the manufacturer’s protocol (STEMCELL Technologies). CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies ...