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Citations for Stemcell Technologies :
51 - 100 of 4280 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... hiPSC colonies were passaged 1:6 every 4 to 7 days with Gentle Cell Dissociation Reagent (STEMCELL Technologies). Genomic integrity was validated by G-band karyotyping (Cell Line Genetics ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies) for definitive endoderm induction ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Developmental Biology 2020Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent and cryopreserved in mFreSR according to manufacturer’s instructions (STEMCELL Technologies). All lines were expanded and stored at low passage number.
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
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bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Microbiology 2020Quote: ... anti-SSEA-4 antibody (STEMCELL Technologies), anti-Sox17 antibody (R&D System) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured as described above (see Primary NSC cell culture) until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: NSCs were cultured as described above (see “Primary NSC cell culture”) in complete activated media until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well in Matrigel (Corning ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μg/mL heparin (STEMCELL Technologies, 07980) and 5 μM ROCK inhibitor (Millipore) ...
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bioRxiv - Bioengineering 2021Quote: Testicular organoids were made by mixing germ and somatic cells at a 1:4 ratio and placing them into AggreWell800™ microwells (STEMCELL Technologies, 34811) overnight ...
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bioRxiv - Genomics 2019Quote: ... and 8 weeks old adult mammary glands (#4 gland only) were dissected and pooled from multiple animals into dissection media (Epicult-B Basal medium (Stem Cell Technologies #05610) supplemented with 5% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 4 µg/mL heparin (STEMCELL Technologies), 480 ng/mL hydrocortisone ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 µg/mL Heparin (StemCell Technologies, cat. 07980), and 400 nL/mL B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 µg/mL Heparin (StemCell Technologies, cat. 07980), and 400 nL/mL B27 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Passaging occurred every 3-4 days using ReLeSR (STEMCELL Technologies) and DPBS 1X (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and banked into 3-4 vials using mFreSR (Stemcell Technologies). Clearance of Sendai virus was confirmed using qPCR (Supplementary Table 4) ...
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bioRxiv - Neuroscience 2022Quote: ... Colonies were passaged every 4 days with ReLeSR (Stemcell technologies) after rinsing with Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cancer Biology 2021Quote: ... in a 3:4 mixture of media (StemCell Technologies # 05620) and Matrigel (BD Bioscience CB-40324) ...
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bioRxiv - Cell Biology 2021Quote: IMR90-4 iPSCs (WiCell) were cultured in mTesR1 (Stemcell Technologies) on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... were passaged 1:4 using ACCUTASE™ (Stemcell technologies, 07920) when confluent and subsequently grown in Astrocyte medium containing leukemia inhibitory factor (LIF ...
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bioRxiv - Neuroscience 2023Quote: ... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... 4*106 iPSCs were seeded per Aggrewell 800 (Stemcell Technologies) in a 24-well plate in 2 ml embryonic body medium (EBM) ...
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bioRxiv - Cancer Biology 2021Quote: ... in the same 3:4 mixture of media (StemCell Technologies #05620) and Matrigel (BD Bioscience CB-40324) ...
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bioRxiv - Microbiology 2023Quote: ... and 4 ug/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
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bioRxiv - Microbiology 2023Quote: ... and 4 ug/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
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bioRxiv - Microbiology 2023Quote: ... and 4 µg/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
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bioRxiv - Cell Biology 2023Quote: ... Heparin 4 µg/mL (Ref 07980, Stemcell technologies, Saint Égrève, France) and Hydrocortisone 480 ng/mL (Ref 07925 ...
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bioRxiv - Genomics 2022Quote: ... 4 mL of 29% Iodoxanol cushion was prepared with OptiPrep (Stemcell Technologies) and diluent medium (250 mM sucrose ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... 480 ng/mL hydrocortisone and 4 µg/mL heparin (STEMCELL Technologies, 07980). ALI cultures were grown in a 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Undifferentiated iPSCs were detached by a 4-min incubation with Accutase (StemCell technologies) and seeded onto Geltrex (Life Technologies)-coated plates at a density of 200,000 cells/cm2 ...