Labshake search
Citations for Stemcell Technologies :
551 - 600 of 2345 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
-
bioRxiv - Neuroscience 2023Quote: ... and 2.8×105 cells/cm2 were plated on Geltrex-coated plates in mTesR+ supplemented with ROCK inhibitor Y-27632 (10uM, StemCell Technologies, 72302). Neuronal induction was started 24h later (day 0 ...
-
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... after expansion the cells were prepared for embryoid body formation using the AggreWell™ EB 400 microwell plate following the manufacturers recommendations (StemCell Technologies). Cells were dissociated in Accutase (Innovative Cell Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... 5.8 × 105 cells in N2/B27 media and supplements were seeded into one well of a 6 well AggreWell™400 culture plate (STEMCELL Technologies) which was coated with 5% Pluronic acid F-127 (Sigma-Aldrich P2443 ...
-
bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
-
bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
-
bioRxiv - Neuroscience 2023Quote: ... 9000 cells were plated per well in a 96-well round-bottom ultra-low attachment plate containing EB seeding medium (Stem Cell Technologies). At day 2 and day 4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
-
bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
-
bioRxiv - Immunology 2024Quote: ... gated for cKit+Sca1+CD11b−IL7Rα− cells and then single cell index sorted into 96-well round-bottom plates containing StemSpan™ SFEM II (Stemcell Technologies) supplemented with 50 ng/mL stem cell factor (in-house ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
-
bioRxiv - Cancer Biology 2024Quote: ... detached WTC-1185 human iPSC cells were transferred to an ultra-low attachment U-bottom 96-well plate (20K cells/well) and cultured in mTeSR Plus media (StemCell Technologies, 5825) supplemented with 10 μM Y-27632 (StemCell Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... Definitive hindgut endoderm was generated as reported.8 Induced cells were harvested and seeded on AggreWellTM400 Microwell Plate (StemCell Technologies Cambridge, MA) at 3.6 million cells in each well for 24 hours ...
-
bioRxiv - Genomics 2024Quote: Freshly isolated monocytes were seeded in 12-well plates with 800 µL of differentiation medium comprising ImmunoCult™-SF Macrophage Medium (StemCell Technologies) supplemented with 50 µg/mL human M-CSF (PeproTech ...
-
bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: RosetteSep and EasySep negative isolation kits (Stemcell Technologies) were used sequentially to isolate CD19+ B-cells by negative selection ...
-
bioRxiv - Microbiology 2020Quote: ... PE positive selection kit (STEMCELL Technologies, https://www.stemcell.com) and Monocyte negative selection kit (STEMCELL Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... CD8 positive selection kit (STEMCELL Technologies, https://www.stemcell.com), PE positive selection kit (STEMCELL Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... CellSep Human CD19+ selection kit (Stem Cell Technologies) was used to enrich for CD19+ cells ...
-
Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Aldefluor kit was bought from Stem cell technologies. Magnetic ChIP kit (Cell Signaling Technology) ...
-
bioRxiv - Bioengineering 2020Quote: Negative selection using RosetteSep kits (Stem Cell Technologies) was adopted to isolate primary human T cells from healthy volunteer donors following leukapheresis ...
-
bioRxiv - Cancer Biology 2022Quote: The ALDEFLUOR kit (StemCell Technologies, Durham, NC, USA) was used to analyze the population with high ALDH enzymatic activity according to the manufacturer’s intructions ...
-
bioRxiv - Genetics 2020Quote: ... STEMdiff Cerebral Organoid kit (Stem Cell Technologies, 08570) was used for organoid culture with timing according to manufacturer’s suggestion and Matrigel embedding as previously described57 ...
-
bioRxiv - Immunology 2022Quote: ... and CD8+ T-cell Isolation Kit (StemCell Technologies #19851 ...
-
bioRxiv - Neuroscience 2022Quote: ... using the STEMdiff cerebral organoid kit (STEMCELL Technologies) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... the STEMdiff Definitive Endoderm Kit (STEMCELL Technologies, 05110), was used for differentiation into endoderm ...
-
bioRxiv - Immunology 2023Quote: ... EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) was used to enrich for CD34+ cells to >90% purity.
-
bioRxiv - Immunology 2023Quote: ... and EasySep Human Monocyte Isolation Kit (StemCell Technologies). In the coculture experiment ...
-
Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... ImmunoCult™ NK cell-expansion kit (StemCell Technologies) was used following the manufacturer’s recommendations to activate isolated NK cells in vitro for 6 days ...
-
bioRxiv - Immunology 2023Quote: ... using a negative selection isolation kit (STEMCELL Technologies), and purity was confirmed to be >97% ...
-
bioRxiv - Cancer Biology 2023Quote: ... with 1% PS and 1 μg/mL doxycycline (StemCell Technologies 72742). Prior to orthotopic injection of primary PDAC cell lines ...
-
bioRxiv - Developmental Biology 2021Quote: ... Scleral punches were initially placed into wells of a 96 well culture plate with 50 μl of N2 medium [Ham’s F-12/DMEM containing 1x N2 supplement (Stem Cell Technologies, Vancouver, BC) until all sclera were obtained ...
-
bioRxiv - Developmental Biology 2021Quote: ... and then seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution following instruction (Stem Cell Technologies, #07010). The culture medium was slightly changed without disturbing aggregates in the V-shape bottom every day and aggregates were collected on day 6 ...
-
bioRxiv - Immunology 2019Quote: ... A total of 649 IgGpos/OVApos hybridoma cells were individually deposited into 96-well plates containing Medium E (StemCell Technologies, Vancouver, BC, Canada). Supernatants were screened by ELISA against OVA seven days after sorting ...
-
bioRxiv - Neuroscience 2019Quote: ... were seeded into one well of a 24-well plate and cultured for four days in StemSpan™ SFEM II PBMC complete medium (STEMCELL Technology, 09655) supplemented with 100 ng/ml SCF (PeproTech ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
-
bioRxiv - Neuroscience 2021Quote: ... The transfected LCLs were then plated into a matrigel-coated six-well plate and maintained in TeSr-E7 xeno-free containing media (Stem Cell Tech Inc.). Twelve days after transfection ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 105 cell/well were plated as small colonies (10-20 cell/colony) on Vitronectin-coated 12-well plates using mTeSR1 media (STEMCELL Technologies, Catalog# 12604013) supplemented with 10 μM ROCK inhibitor (Y-27632 ...
-
bioRxiv - Neuroscience 2022Quote: ... and plated at 10,000 cells per well in PDL-coated 96-well plates in BrainPhys Neuronal Medium (BrainPhys (STEMCELL Technologies; Cat. No. 05790) as the base ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
-
bioRxiv - Pathology 2020Quote: ... human iPS-GFP IMR-90-4 reporter cells were maintained in an undifferentiated state in mTesr1 media on plates coated with Matrigel and were routinely passaged with Versene (StemCell Technologies, Vancouver, CA) in a ratio of 1:6 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
-
bioRxiv - Genetics 2020Quote: ... Transduced cells were expanded to 10 cm plates and 10 vials (2M cells/vial) of each cell line was cryopreserved in CryoStor® (StemCell Technologies, 07930) for further use.
-
bioRxiv - Cancer Biology 2021Quote: ... and then removed from the Parafilm and transferred to wells of a 24-well plate (one form per well) containing 1.5 mL of neural stem cell media (NeuroCultTM; 05700, STEMCELL Technologies, Vancouver, BC, Canada) supplemented with human EGF (20 ng/mL ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were collected by centrifugation at 1300 rpm for 5 minutes and 500 K cells distributed into each well of an AggreWell™800 plate (24 well) (Stem Cell Technologies) as recommended by the manufacturer.
-
bioRxiv - Developmental Biology 2023Quote: ... 7.5-9.0×104 cells were mixed with 0.5 mL culture medium and seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
-
bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.