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Citations for Stemcell Technologies :
151 - 200 of 4608 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
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bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were harvested from SPLNs and MLNs using a CD4+ T cell isolation kit (STEMCELL technologies). Cells (2 × 105 cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... naive CD4+ T cells were isolated using a RoboSep CD4+ Naïve T cell negative selection kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: Splenocyte-derived CD4+ T cells were isolated using Easysep Mouse CD4+ T cell isolation kit (19852, STEMCELL Technologies), and then 2e5 cells/well were plated in a 96 well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cell isolation was performed using EasySep Mouse CD8+ T cell Isolation Kit (#19853, STEMCELL Technologies, USA) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were then isolated using a magnet based CD8 T cell isolation kit (19053, StemCell Technologies) and used immediately for experiments ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells were isolated with EasySep Mouse Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4+ T cells were isolated by negative selection using the EasySep CD4 T cell isolation kit (Stemcell Technologies) and frozen as dry pellet at −80°C.
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated from PBMCs with the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies). Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were purified from P14 spleens using EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies). Cells were then expanded using plate-bound CD3/CD28 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... T-cells were then positively selected using an EasySep T-cell kit (Cat # 17951, STEMCELL Technologies, Vancouver, Canada) and an EasySep Magnet (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies, 17961). Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... with EasySep Human T cell Isolation Kit (STEMCELL Technologies). Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... using the mouse T cell isolation kit (StemCell Technologies), and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Immunology 2021Quote: ... or a custom stem cell kit that removes CD66b+/GPA+/CD3+/CD14+ cells (StemCell Technologies). Enriched fractions were then stained using the fluorescently conjugated antibodies IgD-FITC (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... naïve and memory CD4+ T cells were isolated from PBMCs using EasySep® naïve CD4+ T cell isolation kit and memory CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were isolated from the PBMC fraction using EasySep® naïve CD4+ T cell isolation kits and memory CD4+ T cell enrichment kits (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2021Quote: ... Primary CD4+ or CD8+ T cells were isolated using EasySep Human CD4+ or CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
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bioRxiv - Molecular Biology 2020Quote: ... and LN cells were enriched for CD4+ T cells using the EasySep NHP CD4+ T cell isolation kit from STEMCELL Technologies Canada Inc (Cat# ...
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bioRxiv - Microbiology 2020Quote: A lentiviral vector encoding anti-HIV-1 CAR was transduced and expressed in the enriched total human T cells obtained from fresh peripheral blood mononuclear cells by negative selection (EasySep Human T cell isolation kit, Stemcell Technologies). CAR-T cells were maintained in IMDM supplemented with 20% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Total CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag-2 magnet (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Microbiology 2022Quote: ... primary CD4+ T-cells were negatively enriched using EasySep Human CD4+ T-cell isolation kit (Catalog no. 17952; Stem Cell Technologies) and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were negatively selected from single cell suspensions using the EasySep™ CD4+ T cell isolation kit (Stemcell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Spleens were processed into single cell suspensions and CD4+ T cells were isolated using the EasySep CD4+ T cell isolation kit (StemCell Technologies) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... total CD8+ T cells were first enriched using the EasySep™ Mouse CD8+ T cell isolation kit (Stemcell Technologies) and then CD8+CD44hiCD62Llo central memory T cells purified by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were isolated for intravenous injection using an EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies, Canada). 4×106 isolated T cells were injected intravenously per mouse.
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were isolated from PBMCs via the Easysep Human CD4+ T-cell negative enrichment kit (STEMCELL Technologies). The isolated human CD4+ T cells were cultured at 2.5 × 106 cells/ml in complete RPMI medium (cRPMI) ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from PBMCs with EasySep™ Human T cell Isolation Kit per manufacturer’s instructions (StemCell Technologies). Isolated T cells (0.5-1 × 105 per well ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified from thawed PBMCs using the EasySep Human T cell enrichment kit (STEMCELL Technologies, Cambridge, MA). RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... CD4+ Human T cells were enriched from PBMCs using EasySep™ Human CD4+ T Cell Isolation Kit (StemCell Technologies) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Splenocytes derived from OT1 mice were prepared as described above and naïve CD8+ T cells were isolated using EasySepTM Mouse Naïve CD8+ T cell isolation kit (STEMCELL Technologies) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Enrichment of T cells was performed by EasySep™ mouse T cell isolation kit per manufacturer’s protocol (StemCell Technologies). Retroviral transduction with PSCA-mCAR and/or ffluc and subsequent expansion were performed as previously described.47
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bioRxiv - Immunology 2020Quote: CD4+T-cells were isolated from PBMCs with the EasySep™ human CD4+T-cell enrichment kit (StemCell Technologies). T-cells/PBMCs were added to cultured hepatic epithelial cell lines (Huh-7 ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T cells were enriched by negative selection using the EasySep Human CD4+ T Cell Enrichment Kit (StemCell Technologies). The purity of the CD4+ T cells was evaluated by flow cytometry and was higher than 95% ...
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bioRxiv - Immunology 2019Quote: OT2 T cells were isolated by magnetic beads (EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit, StemCell Technologies) from a single cell suspension of splenocytes ...
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bioRxiv - Microbiology 2021Quote: ... and resting CD4+ T cells (rCD4s) (EasySep™ Human Resting CD4+ T Cell Isolation Kit, Cat # 17962, StemCell Technologies) were isolated from HIV-1 aviremic donors living with HIV-1 ...
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bioRxiv - Immunology 2022Quote: ... Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies). Prior to infection ...
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bioRxiv - Immunology 2022Quote: ... Memory CD4 T cells were isolated from PBMCs using the EasySep Memory CD4+ T cell enrichment kit (StemCell technologies). Total CD4 T cells from PBMCs were isolated using the EasySep Human CD4+ T cell enrichment kit (StemCell technologies) ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were enriched using the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies, Cat. No. 19052) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...